Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF90 and GFF91 are separated by 57 nucleotides GFF91 and GFF92 are separated by 0 nucleotides GFF92 and GFF93 are separated by 2 nucleotides GFF93 and GFF94 are separated by 99 nucleotides
GFF90 - Arsenate reductase (EC 1.20.4.1), at 106,450 to 106,944
GFF90
GFF91 - General secretion pathway protein J, at 107,002 to 107,619
GFF91
GFF92 - KEGG: general secretion pathway protein I, at 107,620 to 107,997
GFF92
GFF93 - General secretion pathway protein H, at 108,000 to 108,494
GFF93
GFF94 - hypothetical protein, at 108,594 to 109,037
GFF94
Position (kb)
107
108 Strain fitness (log2 ratio)
-2
-1
0
1 at 106.955 kb on + strand at 106.955 kb on + strand at 106.957 kb on + strand at 106.957 kb on + strand at 107.621 kb on + strand at 107.622 kb on - strand at 107.684 kb on + strand, within GFF92 at 107.684 kb on + strand, within GFF92 at 107.684 kb on + strand, within GFF92 at 107.684 kb on + strand, within GFF92 at 107.685 kb on - strand, within GFF92 at 107.685 kb on - strand, within GFF92 at 108.013 kb on + strand at 108.014 kb on - strand at 108.014 kb on - strand at 108.497 kb on + strand at 108.498 kb on - strand at 108.498 kb on - strand at 108.498 kb on - strand at 108.498 kb on - strand at 108.558 kb on - strand at 108.633 kb on + strand at 108.633 kb on + strand at 108.633 kb on + strand at 108.634 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 106,955 + -0.1 106,955 + -0.9 106,957 + -0.3 106,957 + +0.4 107,621 + -1.2 107,622 - -0.4 107,684 + GFF92 0.17 -0.6 107,684 + GFF92 0.17 -0.4 107,684 + GFF92 0.17 +0.1 107,684 + GFF92 0.17 -0.7 107,685 - GFF92 0.17 -0.3 107,685 - GFF92 0.17 -0.4 108,013 + +0.2 108,014 - -0.7 108,014 - -0.9 108,497 + -0.9 108,498 - +0.9 108,498 - +0.6 108,498 - +0.1 108,498 - +0.8 108,558 - +1.3 108,633 + -0.7 108,633 + -2.2 108,633 + -2.4 108,634 - -0.2
Or see this region's nucleotide sequence