Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF865 and GFF866 are separated by 60 nucleotides GFF866 and GFF867 are separated by 27 nucleotides GFF867 and GFF868 are separated by 8 nucleotides
GFF865 - hypothetical protein, at 173,138 to 174,190
GFF865
GFF866 - no description, at 174,251 to 174,571
GFF866
GFF867 - Oxidoreductase, GMC family, at 174,599 to 176,224
GFF867
GFF868 - Uracil-DNA glycosylase, family 1, at 176,233 to 177,084
GFF868
Position (kb) 174
175
176
177 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 173.749 kb on - strand, within GFF865 at 173.751 kb on + strand, within GFF865 at 173.751 kb on + strand, within GFF865 at 173.752 kb on - strand, within GFF865 at 174.093 kb on + strand at 174.224 kb on - strand at 174.360 kb on + strand, within GFF866 at 174.361 kb on - strand, within GFF866 at 174.402 kb on + strand, within GFF866 at 174.402 kb on + strand, within GFF866 at 174.402 kb on + strand, within GFF866 at 174.450 kb on + strand, within GFF866 at 174.450 kb on + strand, within GFF866 at 174.450 kb on + strand, within GFF866 at 174.451 kb on - strand, within GFF866 at 174.451 kb on - strand, within GFF866 at 174.508 kb on - strand, within GFF866 at 174.550 kb on - strand at 174.600 kb on + strand at 174.600 kb on + strand at 174.600 kb on + strand at 174.880 kb on - strand, within GFF867 at 174.966 kb on + strand, within GFF867 at 174.966 kb on + strand, within GFF867 at 174.966 kb on + strand, within GFF867 at 174.967 kb on - strand, within GFF867 at 175.111 kb on - strand, within GFF867 at 175.290 kb on + strand, within GFF867 at 175.291 kb on - strand, within GFF867 at 175.317 kb on + strand, within GFF867 at 175.317 kb on + strand, within GFF867 at 175.317 kb on + strand, within GFF867 at 175.318 kb on - strand, within GFF867 at 175.869 kb on + strand, within GFF867 at 175.869 kb on + strand, within GFF867 at 175.870 kb on - strand, within GFF867 at 175.932 kb on + strand, within GFF867 at 175.932 kb on + strand, within GFF867 at 175.932 kb on + strand, within GFF867 at 175.932 kb on + strand, within GFF867 at 175.933 kb on - strand, within GFF867 at 175.986 kb on + strand, within GFF867 at 175.986 kb on + strand, within GFF867 at 176.199 kb on + strand at 176.199 kb on + strand at 176.200 kb on - strand at 176.200 kb on - strand at 176.200 kb on - strand at 176.200 kb on - strand at 176.808 kb on + strand, within GFF868 at 176.809 kb on - strand, within GFF868 at 177.136 kb on - strand at 177.139 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 173,749 - GFF865 0.58 -1.2 173,751 + GFF865 0.58 -1.2 173,751 + GFF865 0.58 -1.0 173,752 - GFF865 0.58 -0.4 174,093 + -0.5 174,224 - -0.0 174,360 + GFF866 0.34 -0.0 174,361 - GFF866 0.34 -0.8 174,402 + GFF866 0.47 -0.7 174,402 + GFF866 0.47 +0.0 174,402 + GFF866 0.47 -0.7 174,450 + GFF866 0.62 +1.0 174,450 + GFF866 0.62 -0.7 174,450 + GFF866 0.62 -0.0 174,451 - GFF866 0.62 +0.1 174,451 - GFF866 0.62 -0.2 174,508 - GFF866 0.80 +1.2 174,550 - +0.3 174,600 + -0.2 174,600 + -0.7 174,600 + -0.8 174,880 - GFF867 0.17 -0.1 174,966 + GFF867 0.23 -0.3 174,966 + GFF867 0.23 -0.9 174,966 + GFF867 0.23 -0.6 174,967 - GFF867 0.23 -0.0 175,111 - GFF867 0.31 -0.2 175,290 + GFF867 0.42 +0.4 175,291 - GFF867 0.43 -0.9 175,317 + GFF867 0.44 -0.0 175,317 + GFF867 0.44 -0.5 175,317 + GFF867 0.44 -0.0 175,318 - GFF867 0.44 -0.4 175,869 + GFF867 0.78 -0.9 175,869 + GFF867 0.78 -1.8 175,870 - GFF867 0.78 -1.0 175,932 + GFF867 0.82 +1.0 175,932 + GFF867 0.82 -0.4 175,932 + GFF867 0.82 -0.4 175,932 + GFF867 0.82 -3.6 175,933 - GFF867 0.82 -0.4 175,986 + GFF867 0.85 -0.0 175,986 + GFF867 0.85 +1.0 176,199 + +0.3 176,199 + -0.2 176,200 - +0.4 176,200 - -0.2 176,200 - -1.3 176,200 - +0.6 176,808 + GFF868 0.67 +1.0 176,809 - GFF868 0.68 -0.9 177,136 - -0.5 177,139 - +1.1
Or see this region's nucleotide sequence