Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7261 and GFF7262 overlap by 4 nucleotides GFF7262 and GFF7263 are separated by 6 nucleotides GFF7263 and GFF7264 are separated by 505 nucleotides
GFF7261 - 2,5-dioxovalerate dehydrogenase (EC 1.2.1.26), at 7,992 to 9,428
GFF7261
GFF7262 - no description, at 9,425 to 9,718
GFF7262
GFF7263 - Malate dehydrogenase (EC 1.1.1.37), at 9,725 to 10,819
GFF7263
GFF7264 - Gluconolactonase (EC 3.1.1.17), at 11,325 to 12,170
GFF7264
Position (kb)
9
10
11 Strain fitness (log2 ratio)
-2
-1
0
1 at 9.590 kb on + strand, within GFF7262 at 9.590 kb on + strand, within GFF7262 at 9.635 kb on + strand, within GFF7262 at 9.683 kb on + strand, within GFF7262 at 9.683 kb on + strand, within GFF7262 at 9.707 kb on + strand at 9.761 kb on + strand at 9.860 kb on + strand, within GFF7263 at 10.047 kb on - strand, within GFF7263 at 10.164 kb on - strand, within GFF7263 at 10.226 kb on - strand, within GFF7263 at 10.568 kb on + strand, within GFF7263 at 10.854 kb on + strand at 10.985 kb on + strand at 10.985 kb on + strand at 11.031 kb on + strand at 11.031 kb on + strand at 11.063 kb on - strand at 11.074 kb on + strand at 11.265 kb on - strand at 11.340 kb on + strand at 11.340 kb on + strand at 11.340 kb on + strand at 11.453 kb on + strand, within GFF7264 at 11.794 kb on - strand, within GFF7264 at 11.794 kb on - strand, within GFF7264
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 9,590 + GFF7262 0.56 -1.4 9,590 + GFF7262 0.56 +0.5 9,635 + GFF7262 0.71 -0.9 9,683 + GFF7262 0.88 -1.6 9,683 + GFF7262 0.88 +0.1 9,707 + +0.0 9,761 + -1.9 9,860 + GFF7263 0.12 -0.6 10,047 - GFF7263 0.29 +0.4 10,164 - GFF7263 0.40 -1.8 10,226 - GFF7263 0.46 -0.4 10,568 + GFF7263 0.77 -0.6 10,854 + -0.8 10,985 + -0.7 10,985 + +0.1 11,031 + +0.3 11,031 + -0.4 11,063 - -0.3 11,074 + -0.6 11,265 - -0.9 11,340 + -0.5 11,340 + +0.2 11,340 + -1.0 11,453 + GFF7264 0.15 -0.6 11,794 - GFF7264 0.55 -0.7 11,794 - GFF7264 0.55 -0.7
Or see this region's nucleotide sequence