Strain Fitness in Variovorax sp. SCN45 around GFF7254

Experiment: M9 with calcium pantothenate 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF7254 and GFF7255 are separated by 228 nucleotides GFF7254 - Mannonate dehydratase (EC 4.2.1.8), at 3 to 959 GFF7254 GFF7255 - BUG/TctC family periplasmic protein, at 1,188 to 2,024 GFF7255 Position (kb) 0 1Strain fitness (log2 ratio) -1 0 1at 0.214 kb on - strand, within GFF7254at 0.214 kb on - strand, within GFF7254at 0.214 kb on - strand, within GFF7254at 0.804 kb on + strand, within GFF7254at 0.805 kb on - strand, within GFF7254at 0.805 kb on - strand, within GFF7254at 0.922 kb on - strandat 1.362 kb on + strand, within GFF7255at 1.515 kb on + strand, within GFF7255at 1.515 kb on + strand, within GFF7255

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL
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214 - GFF7254 0.22 +0.6
214 - GFF7254 0.22 +0.7
214 - GFF7254 0.22 +0.3
804 + GFF7254 0.84 -0.2
805 - GFF7254 0.84 +0.0
805 - GFF7254 0.84 -0.1
922 - -0.6
1,362 + GFF7255 0.21 +0.1
1,515 + GFF7255 0.39 -0.6
1,515 + GFF7255 0.39 -0.8

Or see this region's nucleotide sequence