Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7248 and GFF7249 overlap by 4 nucleotides GFF7249 and GFF7250 are separated by 95 nucleotides GFF7250 and GFF7251 are separated by 350 nucleotides GFF7251 and GFF7252 overlap by 7 nucleotides GFF7252 and GFF7253 are separated by 1 nucleotides
GFF7248 - no description, at 28,408 to 28,563
GFF7248
GFF7249 - no description, at 28,560 to 28,865
GFF7249
GFF7250 - Putative transposase InsK for insertion sequence element IS150, at 28,961 to 29,134
GFF7250
GFF7251 - Mobile element protein, at 29,485 to 29,748
GFF7251
GFF7252 - Mobile element protein, at 29,742 to 30,557
GFF7252
GFF7253 - Mobile element protein, at 30,559 to 30,807
GFF7253
Position (kb) 29
30 Strain fitness (log2 ratio) -2
-1
0
1 at 28.683 kb on + strand, within GFF7249 at 28.841 kb on + strand at 28.863 kb on + strand at 28.864 kb on - strand at 28.864 kb on - strand at 28.897 kb on + strand at 28.898 kb on - strand at 28.907 kb on + strand at 28.907 kb on + strand at 28.954 kb on + strand at 28.955 kb on - strand at 29.054 kb on + strand, within GFF7250 at 29.133 kb on - strand at 29.301 kb on - strand at 29.596 kb on + strand, within GFF7251 at 29.596 kb on + strand, within GFF7251 at 29.597 kb on - strand, within GFF7251 at 30.493 kb on - strand at 30.603 kb on - strand, within GFF7253 at 30.632 kb on + strand, within GFF7253
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 28,683 + GFF7249 0.40 -0.3 28,841 + -0.7 28,863 + -0.3 28,864 - -0.2 28,864 - +0.5 28,897 + +0.3 28,898 - -0.1 28,907 + +0.1 28,907 + -0.2 28,954 + -0.6 28,955 - +0.1 29,054 + GFF7250 0.53 +0.1 29,133 - -0.1 29,301 - -1.9 29,596 + GFF7251 0.42 +0.2 29,596 + GFF7251 0.42 -1.0 29,597 - GFF7251 0.42 -0.7 30,493 - +0.1 30,603 - GFF7253 0.18 -0.3 30,632 + GFF7253 0.29 +0.2
Or see this region's nucleotide sequence