Strain Fitness in Variovorax sp. SCN45 around GFF72

Experiment: M9 with calcium pantothenate 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF70 and GFF71 are separated by 6 nucleotidesGFF71 and GFF72 are separated by 24 nucleotidesGFF72 and GFF73 are separated by 2 nucleotidesGFF73 and GFF74 are separated by 72 nucleotides GFF70 - Mannose-1-phosphate guanylyltransferase (EC 2.7.7.13) / Mannose-6-phosphate isomerase (EC 5.3.1.8), at 86,892 to 88,403 GFF70 GFF71 - Aminoglycoside 3''-nucleotidyltransferase (EC 2.7.7.-) => ANT(3'')-Ia (AadA family), at 88,410 to 89,219 GFF71 GFF72 - D-threo-3-hydroxyaspartate ammonia-lyase (EC 4.3.1.-), at 89,244 to 90,377 GFF72 GFF73 - Probable haloacid dehalogenase, at 90,380 to 91,039 GFF73 GFF74 - Transcriptional regulator, LysR family, at 91,112 to 92,041 GFF74 Position (kb) 89 90 91Strain fitness (log2 ratio) -2 -1 0 1at 88.287 kb on + strandat 88.287 kb on + strandat 88.288 kb on - strandat 88.288 kb on - strandat 88.288 kb on - strandat 88.446 kb on + strandat 88.526 kb on - strand, within GFF71at 88.526 kb on - strand, within GFF71at 88.795 kb on + strand, within GFF71at 88.795 kb on + strand, within GFF71at 88.796 kb on - strand, within GFF71at 89.232 kb on + strandat 89.449 kb on + strand, within GFF72at 89.449 kb on + strand, within GFF72at 89.449 kb on + strand, within GFF72at 89.449 kb on + strand, within GFF72at 89.449 kb on + strand, within GFF72at 89.449 kb on + strand, within GFF72at 89.450 kb on - strand, within GFF72at 89.450 kb on - strand, within GFF72at 89.450 kb on - strand, within GFF72at 89.671 kb on + strand, within GFF72at 89.671 kb on + strand, within GFF72at 89.671 kb on + strand, within GFF72at 89.671 kb on + strand, within GFF72at 89.671 kb on + strand, within GFF72at 89.672 kb on - strand, within GFF72at 89.672 kb on - strand, within GFF72at 89.672 kb on - strand, within GFF72at 90.134 kb on - strand, within GFF72at 90.495 kb on + strand, within GFF73at 90.496 kb on - strand, within GFF73at 90.496 kb on - strand, within GFF73at 90.534 kb on + strand, within GFF73at 90.535 kb on - strand, within GFF73at 90.621 kb on + strand, within GFF73at 90.621 kb on + strand, within GFF73at 90.873 kb on + strand, within GFF73at 90.873 kb on + strand, within GFF73at 90.873 kb on + strand, within GFF73at 90.873 kb on + strand, within GFF73at 90.873 kb on + strand, within GFF73at 90.874 kb on - strand, within GFF73at 90.874 kb on - strand, within GFF73at 90.874 kb on - strand, within GFF73at 90.874 kb on - strand, within GFF73at 90.889 kb on - strand, within GFF73at 90.903 kb on + strand, within GFF73at 91.052 kb on + strandat 91.052 kb on + strandat 91.052 kb on + strandat 91.053 kb on - strandat 91.053 kb on - strandat 91.099 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL
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88,287 + -0.5
88,287 + +1.4
88,288 - -0.4
88,288 - -0.4
88,288 - +0.2
88,446 + -0.6
88,526 - GFF71 0.14 -0.7
88,526 - GFF71 0.14 -0.0
88,795 + GFF71 0.48 -0.3
88,795 + GFF71 0.48 -2.7
88,796 - GFF71 0.48 +1.4
89,232 + -0.5
89,449 + GFF72 0.18 -0.8
89,449 + GFF72 0.18 -0.0
89,449 + GFF72 0.18 +0.7
89,449 + GFF72 0.18 -0.6
89,449 + GFF72 0.18 -0.6
89,449 + GFF72 0.18 +0.8
89,450 - GFF72 0.18 -0.5
89,450 - GFF72 0.18 -0.0
89,450 - GFF72 0.18 +0.2
89,671 + GFF72 0.38 +1.0
89,671 + GFF72 0.38 -1.1
89,671 + GFF72 0.38 +0.3
89,671 + GFF72 0.38 +0.2
89,671 + GFF72 0.38 -0.6
89,672 - GFF72 0.38 +0.3
89,672 - GFF72 0.38 +0.6
89,672 - GFF72 0.38 +0.1
90,134 - GFF72 0.78 +0.8
90,495 + GFF73 0.17 -0.7
90,496 - GFF73 0.18 +0.6
90,496 - GFF73 0.18 -0.2
90,534 + GFF73 0.23 -0.6
90,535 - GFF73 0.23 +0.4
90,621 + GFF73 0.37 -1.4
90,621 + GFF73 0.37 +0.8
90,873 + GFF73 0.75 -0.2
90,873 + GFF73 0.75 -1.2
90,873 + GFF73 0.75 -0.8
90,873 + GFF73 0.75 -0.2
90,873 + GFF73 0.75 -0.2
90,874 - GFF73 0.75 -1.4
90,874 - GFF73 0.75 +0.1
90,874 - GFF73 0.75 +1.3
90,874 - GFF73 0.75 +0.0
90,889 - GFF73 0.77 -0.9
90,903 + GFF73 0.79 -0.6
91,052 + +1.2
91,052 + -0.2
91,052 + -1.9
91,053 - -0.6
91,053 - +1.4
91,099 + -1.7

Or see this region's nucleotide sequence