Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF70 and GFF71 are separated by 6 nucleotides GFF71 and GFF72 are separated by 24 nucleotides GFF72 and GFF73 are separated by 2 nucleotides GFF73 and GFF74 are separated by 72 nucleotides
GFF70 - Mannose-1-phosphate guanylyltransferase (EC 2.7.7.13) / Mannose-6-phosphate isomerase (EC 5.3.1.8), at 86,892 to 88,403
GFF70
GFF71 - Aminoglycoside 3''-nucleotidyltransferase (EC 2.7.7.-) => ANT(3'')-Ia (AadA family), at 88,410 to 89,219
GFF71
GFF72 - D-threo-3-hydroxyaspartate ammonia-lyase (EC 4.3.1.-), at 89,244 to 90,377
GFF72
GFF73 - Probable haloacid dehalogenase, at 90,380 to 91,039
GFF73
GFF74 - Transcriptional regulator, LysR family, at 91,112 to 92,041
GFF74
Position (kb)
89
90
91 Strain fitness (log2 ratio)
-2
-1
0
1 at 88.287 kb on + strand at 88.287 kb on + strand at 88.288 kb on - strand at 88.288 kb on - strand at 88.288 kb on - strand at 88.446 kb on + strand at 88.526 kb on - strand, within GFF71 at 88.526 kb on - strand, within GFF71 at 88.795 kb on + strand, within GFF71 at 88.795 kb on + strand, within GFF71 at 88.796 kb on - strand, within GFF71 at 89.232 kb on + strand at 89.449 kb on + strand, within GFF72 at 89.449 kb on + strand, within GFF72 at 89.449 kb on + strand, within GFF72 at 89.449 kb on + strand, within GFF72 at 89.449 kb on + strand, within GFF72 at 89.449 kb on + strand, within GFF72 at 89.450 kb on - strand, within GFF72 at 89.450 kb on - strand, within GFF72 at 89.450 kb on - strand, within GFF72 at 89.671 kb on + strand, within GFF72 at 89.671 kb on + strand, within GFF72 at 89.671 kb on + strand, within GFF72 at 89.671 kb on + strand, within GFF72 at 89.671 kb on + strand, within GFF72 at 89.672 kb on - strand, within GFF72 at 89.672 kb on - strand, within GFF72 at 89.672 kb on - strand, within GFF72 at 90.134 kb on - strand, within GFF72 at 90.495 kb on + strand, within GFF73 at 90.496 kb on - strand, within GFF73 at 90.496 kb on - strand, within GFF73 at 90.534 kb on + strand, within GFF73 at 90.535 kb on - strand, within GFF73 at 90.621 kb on + strand, within GFF73 at 90.621 kb on + strand, within GFF73 at 90.873 kb on + strand, within GFF73 at 90.873 kb on + strand, within GFF73 at 90.873 kb on + strand, within GFF73 at 90.873 kb on + strand, within GFF73 at 90.873 kb on + strand, within GFF73 at 90.874 kb on - strand, within GFF73 at 90.874 kb on - strand, within GFF73 at 90.874 kb on - strand, within GFF73 at 90.874 kb on - strand, within GFF73 at 90.889 kb on - strand, within GFF73 at 90.903 kb on + strand, within GFF73 at 91.052 kb on + strand at 91.052 kb on + strand at 91.052 kb on + strand at 91.053 kb on - strand at 91.053 kb on - strand at 91.099 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 88,287 + -0.5 88,287 + +1.4 88,288 - -0.4 88,288 - -0.4 88,288 - +0.2 88,446 + -0.6 88,526 - GFF71 0.14 -0.7 88,526 - GFF71 0.14 -0.0 88,795 + GFF71 0.48 -0.3 88,795 + GFF71 0.48 -2.7 88,796 - GFF71 0.48 +1.4 89,232 + -0.5 89,449 + GFF72 0.18 -0.8 89,449 + GFF72 0.18 -0.0 89,449 + GFF72 0.18 +0.7 89,449 + GFF72 0.18 -0.6 89,449 + GFF72 0.18 -0.6 89,449 + GFF72 0.18 +0.8 89,450 - GFF72 0.18 -0.5 89,450 - GFF72 0.18 -0.0 89,450 - GFF72 0.18 +0.2 89,671 + GFF72 0.38 +1.0 89,671 + GFF72 0.38 -1.1 89,671 + GFF72 0.38 +0.3 89,671 + GFF72 0.38 +0.2 89,671 + GFF72 0.38 -0.6 89,672 - GFF72 0.38 +0.3 89,672 - GFF72 0.38 +0.6 89,672 - GFF72 0.38 +0.1 90,134 - GFF72 0.78 +0.8 90,495 + GFF73 0.17 -0.7 90,496 - GFF73 0.18 +0.6 90,496 - GFF73 0.18 -0.2 90,534 + GFF73 0.23 -0.6 90,535 - GFF73 0.23 +0.4 90,621 + GFF73 0.37 -1.4 90,621 + GFF73 0.37 +0.8 90,873 + GFF73 0.75 -0.2 90,873 + GFF73 0.75 -1.2 90,873 + GFF73 0.75 -0.8 90,873 + GFF73 0.75 -0.2 90,873 + GFF73 0.75 -0.2 90,874 - GFF73 0.75 -1.4 90,874 - GFF73 0.75 +0.1 90,874 - GFF73 0.75 +1.3 90,874 - GFF73 0.75 +0.0 90,889 - GFF73 0.77 -0.9 90,903 + GFF73 0.79 -0.6 91,052 + +1.2 91,052 + -0.2 91,052 + -1.9 91,053 - -0.6 91,053 - +1.4 91,099 + -1.7
Or see this region's nucleotide sequence