Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7190 and GFF7191 overlap by 4 nucleotides GFF7191 and GFF7192 are separated by 59 nucleotides
GFF7190 - Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases, at 7,085 to 8,455
GFF7190
GFF7191 - Acyl-CoA dehydrogenase; probable dibenzothiophene desulfurization enzyme, at 8,452 to 9,633
GFF7191
GFF7192 - ABC transporter, substrate-binding protein (cluster 5, nickel/peptides/opines), at 9,693 to 11,291
GFF7192
Position (kb)
8
9
10 Strain fitness (log2 ratio)
-1
0
1 at 7.455 kb on + strand, within GFF7190 at 7.455 kb on + strand, within GFF7190 at 7.456 kb on - strand, within GFF7190 at 7.595 kb on + strand, within GFF7190 at 7.596 kb on - strand, within GFF7190 at 7.658 kb on + strand, within GFF7190 at 7.658 kb on + strand, within GFF7190 at 7.659 kb on - strand, within GFF7190 at 7.659 kb on - strand, within GFF7190 at 7.703 kb on + strand, within GFF7190 at 7.704 kb on - strand, within GFF7190 at 7.704 kb on - strand, within GFF7190 at 7.976 kb on + strand, within GFF7190 at 8.905 kb on + strand, within GFF7191 at 8.905 kb on + strand, within GFF7191 at 8.905 kb on + strand, within GFF7191 at 8.906 kb on - strand, within GFF7191 at 8.981 kb on - strand, within GFF7191 at 8.981 kb on - strand, within GFF7191 at 8.981 kb on - strand, within GFF7191 at 9.844 kb on + strand at 9.845 kb on - strand at 9.880 kb on - strand, within GFF7192 at 10.368 kb on + strand, within GFF7192 at 10.369 kb on - strand, within GFF7192
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 7,455 + GFF7190 0.27 -0.3 7,455 + GFF7190 0.27 -0.9 7,456 - GFF7190 0.27 -0.8 7,595 + GFF7190 0.37 +0.7 7,596 - GFF7190 0.37 -0.5 7,658 + GFF7190 0.42 +1.0 7,658 + GFF7190 0.42 -0.2 7,659 - GFF7190 0.42 +0.8 7,659 - GFF7190 0.42 +0.4 7,703 + GFF7190 0.45 -1.1 7,704 - GFF7190 0.45 -1.0 7,704 - GFF7190 0.45 +0.5 7,976 + GFF7190 0.65 +0.2 8,905 + GFF7191 0.38 -0.3 8,905 + GFF7191 0.38 -0.1 8,905 + GFF7191 0.38 -1.3 8,906 - GFF7191 0.38 +0.1 8,981 - GFF7191 0.45 -1.3 8,981 - GFF7191 0.45 -1.3 8,981 - GFF7191 0.45 +0.0 9,844 + -0.6 9,845 - +0.3 9,880 - GFF7192 0.12 +0.8 10,368 + GFF7192 0.42 -0.1 10,369 - GFF7192 0.42 +0.1
Or see this region's nucleotide sequence