Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7002 and GFF7003 are separated by 41 nucleotides GFF7003 and GFF7004 are separated by 19 nucleotides GFF7004 and GFF7005 overlap by 4 nucleotides
GFF7002 - Flagellar basal-body rod protein FlgG, at 17,948 to 18,736
GFF7002
GFF7003 - Flagellar basal-body rod protein FlgF, at 18,778 to 19,563
GFF7003
GFF7004 - Flagellar biosynthesis protein FlhA, at 19,583 to 21,694
GFF7004
GFF7005 - Flagellar biosynthesis protein FlhB, at 21,691 to 22,785
GFF7005
Position (kb) 19
20
21
22 Strain fitness (log2 ratio) -2
-1
0
1 at 18.721 kb on - strand at 18.721 kb on - strand at 18.940 kb on + strand, within GFF7003 at 18.940 kb on + strand, within GFF7003 at 18.940 kb on + strand, within GFF7003 at 19.034 kb on - strand, within GFF7003 at 19.450 kb on + strand, within GFF7003 at 19.451 kb on - strand, within GFF7003 at 19.451 kb on - strand, within GFF7003 at 19.847 kb on + strand, within GFF7004 at 19.848 kb on - strand, within GFF7004 at 20.365 kb on + strand, within GFF7004 at 20.498 kb on + strand, within GFF7004 at 20.498 kb on + strand, within GFF7004 at 20.499 kb on - strand, within GFF7004 at 20.597 kb on + strand, within GFF7004 at 20.597 kb on + strand, within GFF7004 at 20.598 kb on - strand, within GFF7004 at 20.598 kb on - strand, within GFF7004 at 20.751 kb on + strand, within GFF7004 at 21.009 kb on + strand, within GFF7004 at 21.010 kb on - strand, within GFF7004 at 21.150 kb on + strand, within GFF7004 at 21.151 kb on - strand, within GFF7004 at 21.321 kb on + strand, within GFF7004 at 21.366 kb on + strand, within GFF7004 at 21.367 kb on - strand, within GFF7004 at 21.367 kb on - strand, within GFF7004 at 21.528 kb on + strand at 21.528 kb on + strand at 21.528 kb on + strand at 21.529 kb on - strand at 21.529 kb on - strand at 21.542 kb on + strand at 21.620 kb on + strand at 21.950 kb on + strand, within GFF7005 at 22.072 kb on - strand, within GFF7005 at 22.158 kb on - strand, within GFF7005 at 22.158 kb on - strand, within GFF7005 at 22.244 kb on + strand, within GFF7005 at 22.274 kb on + strand, within GFF7005 at 22.275 kb on - strand, within GFF7005 at 22.275 kb on - strand, within GFF7005 at 22.275 kb on - strand, within GFF7005 at 22.597 kb on + strand, within GFF7005
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 18,721 - -0.8 18,721 - -0.1 18,940 + GFF7003 0.21 +0.6 18,940 + GFF7003 0.21 +1.5 18,940 + GFF7003 0.21 -0.9 19,034 - GFF7003 0.33 -0.4 19,450 + GFF7003 0.85 +0.2 19,451 - GFF7003 0.86 -0.3 19,451 - GFF7003 0.86 -0.1 19,847 + GFF7004 0.12 -0.5 19,848 - GFF7004 0.13 -0.1 20,365 + GFF7004 0.37 +0.4 20,498 + GFF7004 0.43 -0.7 20,498 + GFF7004 0.43 -0.2 20,499 - GFF7004 0.43 +1.0 20,597 + GFF7004 0.48 -0.6 20,597 + GFF7004 0.48 -0.9 20,598 - GFF7004 0.48 +0.7 20,598 - GFF7004 0.48 +1.4 20,751 + GFF7004 0.55 -0.3 21,009 + GFF7004 0.68 -1.4 21,010 - GFF7004 0.68 +0.8 21,150 + GFF7004 0.74 +0.7 21,151 - GFF7004 0.74 +0.3 21,321 + GFF7004 0.82 +0.3 21,366 + GFF7004 0.84 -0.8 21,367 - GFF7004 0.84 -1.6 21,367 - GFF7004 0.84 +0.4 21,528 + -1.3 21,528 + -1.0 21,528 + -0.3 21,529 - +0.6 21,529 - -0.5 21,542 + -0.2 21,620 + +0.0 21,950 + GFF7005 0.24 +0.2 22,072 - GFF7005 0.35 -0.4 22,158 - GFF7005 0.43 +1.3 22,158 - GFF7005 0.43 +0.2 22,244 + GFF7005 0.51 +0.9 22,274 + GFF7005 0.53 -0.8 22,275 - GFF7005 0.53 -2.0 22,275 - GFF7005 0.53 +0.0 22,275 - GFF7005 0.53 -0.8 22,597 + GFF7005 0.83 -0.1
Or see this region's nucleotide sequence