Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6728 and GFF6729 are separated by 33 nucleotides GFF6729 and GFF6730 overlap by 4 nucleotides GFF6730 and GFF6731 overlap by 8 nucleotides GFF6731 and GFF6732 overlap by 4 nucleotides
GFF6728 - Probable VANILLIN dehydrogenase oxidoreductase protein (EC 1.-.-.-), at 38,557 to 40,008
GFF6728
GFF6729 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 40,042 to 40,755
GFF6729
GFF6730 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 40,752 to 41,504
GFF6730
GFF6731 - Nucleoside ABC transporter, permease protein 1, at 41,497 to 42,435
GFF6731
GFF6732 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 42,432 to 43,310
GFF6732
Position (kb)
40
41
42 Strain fitness (log2 ratio)
-2
-1
0
1 at 40.136 kb on + strand, within GFF6729 at 40.709 kb on + strand at 40.876 kb on + strand, within GFF6730 at 40.876 kb on + strand, within GFF6730 at 40.877 kb on - strand, within GFF6730 at 40.877 kb on - strand, within GFF6730 at 41.267 kb on - strand, within GFF6730 at 41.267 kb on - strand, within GFF6730 at 41.267 kb on - strand, within GFF6730 at 41.267 kb on - strand, within GFF6730 at 41.567 kb on + strand at 41.610 kb on - strand, within GFF6731 at 41.610 kb on - strand, within GFF6731 at 41.765 kb on + strand, within GFF6731 at 41.766 kb on - strand, within GFF6731 at 41.766 kb on - strand, within GFF6731 at 41.978 kb on + strand, within GFF6731 at 41.978 kb on + strand, within GFF6731 at 41.979 kb on - strand, within GFF6731 at 42.175 kb on + strand, within GFF6731 at 42.175 kb on + strand, within GFF6731 at 42.176 kb on - strand, within GFF6731 at 42.221 kb on + strand, within GFF6731 at 42.221 kb on + strand, within GFF6731 at 42.222 kb on - strand, within GFF6731 at 42.222 kb on - strand, within GFF6731 at 42.311 kb on + strand, within GFF6731 at 42.312 kb on - strand, within GFF6731 at 42.490 kb on + strand at 42.490 kb on + strand at 42.491 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 40,136 + GFF6729 0.13 +0.8 40,709 + -1.3 40,876 + GFF6730 0.16 -0.6 40,876 + GFF6730 0.16 -0.8 40,877 - GFF6730 0.17 +0.0 40,877 - GFF6730 0.17 +0.5 41,267 - GFF6730 0.68 -1.4 41,267 - GFF6730 0.68 -1.7 41,267 - GFF6730 0.68 -0.3 41,267 - GFF6730 0.68 -0.5 41,567 + -0.8 41,610 - GFF6731 0.12 -1.6 41,610 - GFF6731 0.12 +1.6 41,765 + GFF6731 0.29 +0.2 41,766 - GFF6731 0.29 -0.8 41,766 - GFF6731 0.29 +0.8 41,978 + GFF6731 0.51 +1.1 41,978 + GFF6731 0.51 +1.0 41,979 - GFF6731 0.51 -0.4 42,175 + GFF6731 0.72 -1.6 42,175 + GFF6731 0.72 -0.2 42,176 - GFF6731 0.72 -0.4 42,221 + GFF6731 0.77 -0.3 42,221 + GFF6731 0.77 -0.1 42,222 - GFF6731 0.77 +0.0 42,222 - GFF6731 0.77 +0.2 42,311 + GFF6731 0.87 -1.9 42,312 - GFF6731 0.87 +0.3 42,490 + -0.2 42,490 + +0.1 42,491 - +0.2
Or see this region's nucleotide sequence