Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6712 and GFF6713 are separated by 67 nucleotides GFF6713 and GFF6714 overlap by 4 nucleotides
GFF6712 - ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines), at 20,219 to 21,259
GFF6712
GFF6713 - ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines), at 21,327 to 22,304
GFF6713
GFF6714 - Putative cryptic D-serine deaminase (EC 4.3.1.18), at 22,301 to 23,617
GFF6714
Position (kb)
21
22
23 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 20.939 kb on + strand, within GFF6712 at 21.002 kb on + strand, within GFF6712 at 21.003 kb on - strand, within GFF6712 at 21.312 kb on + strand at 21.313 kb on - strand at 21.313 kb on - strand at 21.313 kb on - strand at 21.313 kb on - strand at 21.313 kb on - strand at 21.313 kb on - strand at 21.313 kb on - strand at 21.373 kb on - strand at 21.485 kb on - strand, within GFF6713 at 21.485 kb on - strand, within GFF6713 at 21.485 kb on - strand, within GFF6713 at 22.002 kb on + strand, within GFF6713 at 22.003 kb on - strand, within GFF6713 at 22.003 kb on - strand, within GFF6713 at 22.066 kb on - strand, within GFF6713 at 22.257 kb on + strand at 22.258 kb on - strand at 22.258 kb on - strand at 22.258 kb on - strand at 22.269 kb on - strand at 22.666 kb on + strand, within GFF6714 at 22.822 kb on - strand, within GFF6714 at 22.822 kb on - strand, within GFF6714 at 22.822 kb on - strand, within GFF6714 at 22.952 kb on + strand, within GFF6714 at 22.952 kb on + strand, within GFF6714 at 22.952 kb on + strand, within GFF6714 at 22.998 kb on - strand, within GFF6714 at 22.998 kb on - strand, within GFF6714 at 23.177 kb on + strand, within GFF6714 at 23.177 kb on + strand, within GFF6714 at 23.177 kb on + strand, within GFF6714 at 23.178 kb on - strand, within GFF6714 at 23.178 kb on - strand, within GFF6714 at 23.178 kb on - strand, within GFF6714
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 20,939 + GFF6712 0.69 -0.1 21,002 + GFF6712 0.75 -0.3 21,003 - GFF6712 0.75 -0.0 21,312 + +0.4 21,313 - -1.5 21,313 - -0.5 21,313 - +1.4 21,313 - -0.3 21,313 - -0.5 21,313 - -0.0 21,313 - -2.0 21,373 - +0.1 21,485 - GFF6713 0.16 -0.3 21,485 - GFF6713 0.16 -0.7 21,485 - GFF6713 0.16 -0.2 22,002 + GFF6713 0.69 -0.6 22,003 - GFF6713 0.69 -0.5 22,003 - GFF6713 0.69 -0.7 22,066 - GFF6713 0.76 -0.8 22,257 + -0.2 22,258 - -0.6 22,258 - +0.2 22,258 - -0.3 22,269 - +0.8 22,666 + GFF6714 0.28 -0.0 22,822 - GFF6714 0.40 -0.0 22,822 - GFF6714 0.40 -0.5 22,822 - GFF6714 0.40 +0.3 22,952 + GFF6714 0.49 +0.4 22,952 + GFF6714 0.49 -1.0 22,952 + GFF6714 0.49 -0.6 22,998 - GFF6714 0.53 -1.0 22,998 - GFF6714 0.53 -1.5 23,177 + GFF6714 0.67 +0.5 23,177 + GFF6714 0.67 -0.9 23,177 + GFF6714 0.67 +1.8 23,178 - GFF6714 0.67 -0.7 23,178 - GFF6714 0.67 +0.2 23,178 - GFF6714 0.67 -0.5
Or see this region's nucleotide sequence