Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6687 and GFF6688 are separated by 63 nucleotides GFF6688 and GFF6689 are separated by 20 nucleotides
GFF6687 - hypothetical protein, at 64,124 to 64,732
GFF6687
GFF6688 - cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases, at 64,796 to 65,410
GFF6688
GFF6689 - LSU ribosomal maturation GTPase RbgA (B. subtilis YlqF), at 65,431 to 66,393
GFF6689
Position (kb) 64
65
66 Strain fitness (log2 ratio) -2
-1
0
1
2 at 63.838 kb on - strand at 63.996 kb on + strand at 63.996 kb on + strand at 63.996 kb on + strand at 63.996 kb on + strand at 63.996 kb on + strand at 63.997 kb on - strand at 63.997 kb on - strand at 64.011 kb on + strand at 64.011 kb on + strand at 64.011 kb on + strand at 64.011 kb on + strand at 64.011 kb on + strand at 64.011 kb on + strand at 64.011 kb on + strand at 64.011 kb on + strand at 64.011 kb on + strand at 64.011 kb on + strand at 64.011 kb on + strand at 64.012 kb on - strand at 64.012 kb on - strand at 64.012 kb on - strand at 64.012 kb on - strand at 64.012 kb on - strand at 64.012 kb on - strand at 64.012 kb on - strand at 64.012 kb on - strand at 64.044 kb on + strand at 64.044 kb on + strand at 64.045 kb on - strand at 64.045 kb on - strand at 64.045 kb on - strand at 64.045 kb on - strand at 64.045 kb on - strand at 64.045 kb on - strand at 64.045 kb on - strand at 64.125 kb on + strand at 64.125 kb on + strand at 64.126 kb on - strand at 64.377 kb on + strand, within GFF6687 at 64.378 kb on - strand, within GFF6687 at 64.378 kb on - strand, within GFF6687 at 64.378 kb on - strand, within GFF6687 at 64.378 kb on - strand, within GFF6687 at 64.378 kb on - strand, within GFF6687 at 64.432 kb on - strand, within GFF6687 at 64.590 kb on + strand, within GFF6687 at 64.590 kb on + strand, within GFF6687 at 64.590 kb on + strand, within GFF6687 at 64.590 kb on + strand, within GFF6687 at 64.591 kb on - strand, within GFF6687 at 64.591 kb on - strand, within GFF6687 at 64.591 kb on - strand, within GFF6687 at 64.591 kb on - strand, within GFF6687 at 64.591 kb on - strand, within GFF6687 at 64.638 kb on + strand, within GFF6687 at 64.638 kb on + strand, within GFF6687 at 64.638 kb on + strand, within GFF6687 at 64.638 kb on + strand, within GFF6687 at 64.638 kb on + strand, within GFF6687 at 64.638 kb on + strand, within GFF6687 at 64.639 kb on - strand, within GFF6687 at 64.639 kb on - strand, within GFF6687 at 64.639 kb on - strand, within GFF6687 at 64.639 kb on - strand, within GFF6687 at 64.639 kb on - strand, within GFF6687 at 64.639 kb on - strand, within GFF6687 at 64.639 kb on - strand, within GFF6687 at 64.639 kb on - strand, within GFF6687 at 64.797 kb on + strand at 64.798 kb on - strand at 64.807 kb on + strand at 64.807 kb on + strand at 64.860 kb on + strand, within GFF6688 at 64.860 kb on + strand, within GFF6688 at 64.861 kb on - strand, within GFF6688 at 64.861 kb on - strand, within GFF6688 at 64.861 kb on - strand, within GFF6688 at 64.927 kb on - strand, within GFF6688 at 65.202 kb on + strand, within GFF6688 at 65.202 kb on + strand, within GFF6688 at 65.202 kb on + strand, within GFF6688 at 65.202 kb on + strand, within GFF6688 at 65.202 kb on + strand, within GFF6688 at 65.202 kb on + strand, within GFF6688 at 65.202 kb on + strand, within GFF6688 at 65.203 kb on - strand, within GFF6688 at 66.397 kb on + strand at 66.397 kb on + strand at 66.398 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 63,838 - +0.1 63,996 + -0.2 63,996 + +0.5 63,996 + -0.1 63,996 + -0.5 63,996 + +0.2 63,997 - +0.6 63,997 - -0.5 64,011 + -1.3 64,011 + +0.1 64,011 + -0.3 64,011 + -0.3 64,011 + -0.8 64,011 + +0.5 64,011 + +0.1 64,011 + +0.9 64,011 + -1.1 64,011 + -0.1 64,011 + -1.1 64,012 - +0.2 64,012 - +0.1 64,012 - -0.1 64,012 - -1.8 64,012 - -0.5 64,012 - -1.1 64,012 - -1.2 64,012 - +2.5 64,044 + +0.3 64,044 + -0.3 64,045 - -0.0 64,045 - -0.1 64,045 - -0.5 64,045 - +0.3 64,045 - -0.3 64,045 - +0.0 64,045 - -0.2 64,125 + +0.4 64,125 + -0.1 64,126 - +0.1 64,377 + GFF6687 0.42 -0.4 64,378 - GFF6687 0.42 +0.0 64,378 - GFF6687 0.42 +0.2 64,378 - GFF6687 0.42 +1.7 64,378 - GFF6687 0.42 -1.5 64,378 - GFF6687 0.42 -0.7 64,432 - GFF6687 0.51 -1.2 64,590 + GFF6687 0.77 +0.3 64,590 + GFF6687 0.77 -0.7 64,590 + GFF6687 0.77 +0.2 64,590 + GFF6687 0.77 -0.5 64,591 - GFF6687 0.77 -0.1 64,591 - GFF6687 0.77 -0.1 64,591 - GFF6687 0.77 -0.1 64,591 - GFF6687 0.77 -0.3 64,591 - GFF6687 0.77 -0.7 64,638 + GFF6687 0.84 -1.2 64,638 + GFF6687 0.84 -0.1 64,638 + GFF6687 0.84 -0.1 64,638 + GFF6687 0.84 -0.3 64,638 + GFF6687 0.84 +0.6 64,638 + GFF6687 0.84 +0.7 64,639 - GFF6687 0.85 +0.0 64,639 - GFF6687 0.85 -1.0 64,639 - GFF6687 0.85 -0.1 64,639 - GFF6687 0.85 -2.1 64,639 - GFF6687 0.85 +0.7 64,639 - GFF6687 0.85 -2.5 64,639 - GFF6687 0.85 +0.9 64,639 - GFF6687 0.85 +1.1 64,797 + +0.2 64,798 - -0.3 64,807 + -1.0 64,807 + -0.0 64,860 + GFF6688 0.10 +0.3 64,860 + GFF6688 0.10 -0.1 64,861 - GFF6688 0.11 -0.5 64,861 - GFF6688 0.11 +0.4 64,861 - GFF6688 0.11 -0.7 64,927 - GFF6688 0.21 +0.1 65,202 + GFF6688 0.66 -0.9 65,202 + GFF6688 0.66 +0.0 65,202 + GFF6688 0.66 -0.7 65,202 + GFF6688 0.66 -1.1 65,202 + GFF6688 0.66 -0.8 65,202 + GFF6688 0.66 -1.5 65,202 + GFF6688 0.66 -1.0 65,203 - GFF6688 0.66 +0.3 66,397 + -0.7 66,397 + +1.4 66,398 - -0.2
Or see this region's nucleotide sequence