Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6088 and GFF6089 are separated by 149 nucleotides GFF6089 and GFF6090 are separated by 3 nucleotides GFF6090 and GFF6091 overlap by 4 nucleotides
GFF6088 - Phenylacetic acid degradation protein PaaY, at 149,385 to 149,987
GFF6088
GFF6089 - 1,2-epoxyphenylacetyl-CoA isomerase (EC 5.3.3.18), at 150,137 to 150,940
GFF6089
GFF6090 - Acyl-coenzyme A thioesterase PaaD (Pse.pu.) (E. coli PaaI), at 150,944 to 151,402
GFF6090
GFF6091 - Phenylacetate-coenzyme A ligase (EC 6.2.1.30), at 151,399 to 152,709
GFF6091
Position (kb) 150
151
152 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 149.977 kb on + strand at 149.977 kb on + strand at 149.977 kb on + strand at 149.978 kb on - strand at 149.978 kb on - strand at 149.978 kb on - strand at 149.978 kb on - strand at 150.043 kb on + strand at 150.043 kb on + strand at 150.043 kb on + strand at 150.044 kb on - strand at 150.044 kb on - strand at 150.044 kb on - strand at 150.044 kb on - strand at 150.046 kb on + strand at 150.046 kb on + strand at 150.046 kb on + strand at 150.046 kb on + strand at 150.046 kb on + strand at 150.046 kb on + strand at 150.046 kb on + strand at 150.047 kb on - strand at 150.047 kb on - strand at 150.047 kb on - strand at 150.153 kb on + strand at 150.153 kb on + strand at 150.154 kb on - strand at 150.154 kb on - strand at 150.154 kb on - strand at 150.154 kb on - strand at 150.154 kb on - strand at 150.178 kb on - strand at 150.178 kb on - strand at 150.410 kb on + strand, within GFF6089 at 150.410 kb on + strand, within GFF6089 at 150.410 kb on + strand, within GFF6089 at 150.410 kb on + strand, within GFF6089 at 150.410 kb on + strand, within GFF6089 at 150.411 kb on - strand, within GFF6089 at 150.411 kb on - strand, within GFF6089 at 150.411 kb on - strand, within GFF6089 at 150.413 kb on + strand, within GFF6089 at 150.413 kb on + strand, within GFF6089 at 150.413 kb on + strand, within GFF6089 at 150.413 kb on + strand, within GFF6089 at 150.413 kb on + strand, within GFF6089 at 150.413 kb on + strand, within GFF6089 at 150.413 kb on + strand, within GFF6089 at 150.414 kb on - strand, within GFF6089 at 150.414 kb on - strand, within GFF6089 at 150.414 kb on - strand, within GFF6089 at 150.414 kb on - strand, within GFF6089 at 150.414 kb on - strand, within GFF6089 at 150.414 kb on - strand, within GFF6089 at 150.983 kb on + strand at 150.983 kb on + strand at 150.983 kb on + strand at 150.984 kb on - strand at 151.176 kb on - strand, within GFF6090 at 151.176 kb on - strand, within GFF6090 at 151.176 kb on - strand, within GFF6090 at 151.292 kb on + strand, within GFF6090 at 151.292 kb on + strand, within GFF6090 at 151.292 kb on + strand, within GFF6090 at 151.292 kb on + strand, within GFF6090 at 151.292 kb on + strand, within GFF6090 at 151.293 kb on - strand, within GFF6090 at 151.293 kb on - strand, within GFF6090 at 151.293 kb on - strand, within GFF6090 at 151.293 kb on - strand, within GFF6090 at 151.293 kb on - strand, within GFF6090 at 151.293 kb on - strand, within GFF6090 at 151.293 kb on - strand, within GFF6090 at 151.352 kb on + strand, within GFF6090 at 151.528 kb on + strand at 151.633 kb on + strand, within GFF6091 at 151.633 kb on + strand, within GFF6091 at 151.633 kb on + strand, within GFF6091 at 151.633 kb on + strand, within GFF6091 at 151.633 kb on + strand, within GFF6091 at 151.634 kb on - strand, within GFF6091 at 151.679 kb on + strand, within GFF6091 at 151.680 kb on - strand, within GFF6091 at 151.723 kb on + strand, within GFF6091 at 151.817 kb on - strand, within GFF6091 at 151.817 kb on - strand, within GFF6091 at 151.822 kb on + strand, within GFF6091 at 151.856 kb on - strand, within GFF6091 at 151.969 kb on + strand, within GFF6091 at 151.969 kb on + strand, within GFF6091 at 151.969 kb on + strand, within GFF6091 at 151.969 kb on + strand, within GFF6091 at 151.970 kb on - strand, within GFF6091 at 151.970 kb on - strand, within GFF6091 at 151.970 kb on - strand, within GFF6091 at 151.970 kb on - strand, within GFF6091 at 151.970 kb on - strand, within GFF6091 at 151.970 kb on - strand, within GFF6091 at 152.119 kb on + strand, within GFF6091 at 152.119 kb on + strand, within GFF6091 at 152.119 kb on + strand, within GFF6091 at 152.119 kb on + strand, within GFF6091 at 152.120 kb on - strand, within GFF6091 at 152.206 kb on + strand, within GFF6091 at 152.207 kb on - strand, within GFF6091 at 152.367 kb on + strand, within GFF6091
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 149,977 + -0.2 149,977 + -1.1 149,977 + +0.9 149,978 - +0.3 149,978 - +0.4 149,978 - +0.2 149,978 - -0.0 150,043 + -1.7 150,043 + -1.6 150,043 + -2.0 150,044 - -0.1 150,044 - -0.6 150,044 - -2.4 150,044 - +0.1 150,046 + -0.3 150,046 + -1.8 150,046 + -0.7 150,046 + +0.0 150,046 + +0.0 150,046 + -0.2 150,046 + -1.8 150,047 - +0.0 150,047 - -0.0 150,047 - -1.3 150,153 + -0.0 150,153 + -0.1 150,154 - +0.4 150,154 - +0.3 150,154 - -0.7 150,154 - -0.4 150,154 - +0.2 150,178 - -0.2 150,178 - -0.0 150,410 + GFF6089 0.34 -0.1 150,410 + GFF6089 0.34 +0.0 150,410 + GFF6089 0.34 -1.8 150,410 + GFF6089 0.34 -0.7 150,410 + GFF6089 0.34 -0.1 150,411 - GFF6089 0.34 -0.6 150,411 - GFF6089 0.34 -0.0 150,411 - GFF6089 0.34 -0.6 150,413 + GFF6089 0.34 +0.6 150,413 + GFF6089 0.34 +1.8 150,413 + GFF6089 0.34 +1.3 150,413 + GFF6089 0.34 +0.8 150,413 + GFF6089 0.34 +0.3 150,413 + GFF6089 0.34 -2.4 150,413 + GFF6089 0.34 +0.2 150,414 - GFF6089 0.34 -0.3 150,414 - GFF6089 0.34 -0.3 150,414 - GFF6089 0.34 -1.6 150,414 - GFF6089 0.34 -0.2 150,414 - GFF6089 0.34 -3.4 150,414 - GFF6089 0.34 -0.2 150,983 + +0.8 150,983 + -0.9 150,983 + -1.0 150,984 - +0.1 151,176 - GFF6090 0.51 +0.5 151,176 - GFF6090 0.51 +0.0 151,176 - GFF6090 0.51 +0.5 151,292 + GFF6090 0.76 +0.2 151,292 + GFF6090 0.76 -2.0 151,292 + GFF6090 0.76 +1.2 151,292 + GFF6090 0.76 -0.7 151,292 + GFF6090 0.76 -0.1 151,293 - GFF6090 0.76 -1.4 151,293 - GFF6090 0.76 -0.4 151,293 - GFF6090 0.76 -0.3 151,293 - GFF6090 0.76 -0.7 151,293 - GFF6090 0.76 -1.3 151,293 - GFF6090 0.76 -0.3 151,293 - GFF6090 0.76 -1.2 151,352 + GFF6090 0.89 -1.3 151,528 + -0.1 151,633 + GFF6091 0.18 +0.3 151,633 + GFF6091 0.18 -0.5 151,633 + GFF6091 0.18 -0.4 151,633 + GFF6091 0.18 -0.5 151,633 + GFF6091 0.18 +0.4 151,634 - GFF6091 0.18 -0.3 151,679 + GFF6091 0.21 -0.6 151,680 - GFF6091 0.21 -0.1 151,723 + GFF6091 0.25 +0.8 151,817 - GFF6091 0.32 +0.3 151,817 - GFF6091 0.32 +0.2 151,822 + GFF6091 0.32 -0.5 151,856 - GFF6091 0.35 -1.2 151,969 + GFF6091 0.43 -0.5 151,969 + GFF6091 0.43 +0.1 151,969 + GFF6091 0.43 +0.2 151,969 + GFF6091 0.43 +0.0 151,970 - GFF6091 0.44 +0.2 151,970 - GFF6091 0.44 -0.7 151,970 - GFF6091 0.44 -0.3 151,970 - GFF6091 0.44 +0.5 151,970 - GFF6091 0.44 +0.5 151,970 - GFF6091 0.44 -0.8 152,119 + GFF6091 0.55 -0.4 152,119 + GFF6091 0.55 -2.1 152,119 + GFF6091 0.55 -0.0 152,119 + GFF6091 0.55 +0.4 152,120 - GFF6091 0.55 -0.7 152,206 + GFF6091 0.62 -0.7 152,207 - GFF6091 0.62 -0.5 152,367 + GFF6091 0.74 -1.5
Or see this region's nucleotide sequence