Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6030 and GFF6031 are separated by 1 nucleotides GFF6031 and GFF6032 are separated by 20 nucleotides GFF6032 and GFF6033 are separated by 10 nucleotides
GFF6030 - Branched-chain amino acid ABC transporter, ATP-binding protein LivF (TC 3.A.1.4.1), at 90,664 to 91,440
GFF6030
GFF6031 - Branched-chain amino acid ABC transporter, ATP-binding protein LivG (TC 3.A.1.4.1), at 91,442 to 92,221
GFF6031
GFF6032 - Branched-chain amino acid ABC transporter, permease protein LivM (TC 3.A.1.4.1), at 92,242 to 93,450
GFF6032
GFF6033 - Branched-chain amino acid ABC transporter, permease protein LivH (TC 3.A.1.4.1), at 93,461 to 94,390
GFF6033
Position (kb)
92
93
94 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 91.464 kb on + strand at 91.465 kb on - strand at 91.465 kb on - strand at 91.465 kb on - strand at 91.530 kb on + strand, within GFF6031 at 91.530 kb on + strand, within GFF6031 at 91.531 kb on - strand, within GFF6031 at 91.531 kb on - strand, within GFF6031 at 91.531 kb on - strand, within GFF6031 at 91.531 kb on - strand, within GFF6031 at 91.746 kb on + strand, within GFF6031 at 91.767 kb on + strand, within GFF6031 at 91.767 kb on + strand, within GFF6031 at 91.794 kb on + strand, within GFF6031 at 91.794 kb on + strand, within GFF6031 at 91.794 kb on + strand, within GFF6031 at 91.795 kb on - strand, within GFF6031 at 91.795 kb on - strand, within GFF6031 at 91.795 kb on - strand, within GFF6031 at 92.007 kb on + strand, within GFF6031 at 92.049 kb on + strand, within GFF6031 at 92.049 kb on + strand, within GFF6031 at 92.050 kb on - strand, within GFF6031 at 92.050 kb on - strand, within GFF6031 at 92.112 kb on + strand, within GFF6031 at 92.113 kb on - strand, within GFF6031 at 92.113 kb on - strand, within GFF6031 at 92.299 kb on - strand at 92.528 kb on + strand, within GFF6032 at 92.528 kb on + strand, within GFF6032 at 92.528 kb on + strand, within GFF6032 at 92.528 kb on + strand, within GFF6032 at 92.528 kb on + strand, within GFF6032 at 92.528 kb on + strand, within GFF6032 at 92.874 kb on - strand, within GFF6032 at 92.874 kb on - strand, within GFF6032 at 92.874 kb on - strand, within GFF6032 at 92.874 kb on - strand, within GFF6032 at 92.874 kb on - strand, within GFF6032 at 92.874 kb on - strand, within GFF6032 at 92.874 kb on - strand, within GFF6032 at 92.876 kb on + strand, within GFF6032 at 92.876 kb on + strand, within GFF6032 at 92.876 kb on + strand, within GFF6032 at 92.876 kb on + strand, within GFF6032 at 92.876 kb on + strand, within GFF6032 at 92.877 kb on - strand, within GFF6032 at 92.877 kb on - strand, within GFF6032 at 92.879 kb on + strand, within GFF6032 at 92.879 kb on + strand, within GFF6032 at 92.879 kb on + strand, within GFF6032 at 92.880 kb on - strand, within GFF6032 at 92.880 kb on - strand, within GFF6032 at 92.880 kb on - strand, within GFF6032 at 92.880 kb on - strand, within GFF6032 at 93.065 kb on + strand, within GFF6032 at 93.066 kb on - strand, within GFF6032 at 93.240 kb on - strand, within GFF6032 at 93.240 kb on - strand, within GFF6032 at 93.266 kb on + strand, within GFF6032 at 93.266 kb on + strand, within GFF6032 at 93.266 kb on + strand, within GFF6032 at 93.266 kb on + strand, within GFF6032 at 93.266 kb on + strand, within GFF6032 at 93.266 kb on + strand, within GFF6032 at 93.266 kb on + strand, within GFF6032 at 93.266 kb on + strand, within GFF6032 at 93.267 kb on - strand, within GFF6032 at 93.267 kb on - strand, within GFF6032 at 93.267 kb on - strand, within GFF6032 at 93.267 kb on - strand, within GFF6032 at 93.290 kb on + strand, within GFF6032 at 93.329 kb on + strand, within GFF6032 at 93.329 kb on + strand, within GFF6032 at 93.330 kb on - strand at 93.330 kb on - strand at 93.330 kb on - strand at 93.422 kb on + strand at 93.422 kb on + strand at 93.422 kb on + strand at 93.423 kb on - strand at 93.423 kb on - strand at 93.423 kb on - strand at 93.462 kb on + strand at 93.552 kb on + strand at 93.552 kb on + strand at 93.552 kb on + strand at 93.552 kb on + strand at 93.553 kb on - strand at 93.553 kb on - strand at 93.733 kb on - strand, within GFF6033 at 93.744 kb on + strand, within GFF6033 at 93.744 kb on + strand, within GFF6033 at 93.744 kb on + strand, within GFF6033 at 93.744 kb on + strand, within GFF6033 at 93.744 kb on + strand, within GFF6033 at 93.745 kb on - strand, within GFF6033 at 93.745 kb on - strand, within GFF6033 at 93.745 kb on - strand, within GFF6033 at 93.745 kb on - strand, within GFF6033 at 93.745 kb on - strand, within GFF6033 at 93.745 kb on - strand, within GFF6033 at 93.745 kb on - strand, within GFF6033 at 93.745 kb on - strand, within GFF6033 at 93.882 kb on + strand, within GFF6033 at 93.882 kb on + strand, within GFF6033 at 93.883 kb on - strand, within GFF6033 at 93.883 kb on - strand, within GFF6033 at 93.883 kb on - strand, within GFF6033 at 93.883 kb on - strand, within GFF6033 at 94.003 kb on - strand, within GFF6033 at 94.265 kb on + strand, within GFF6033 at 94.265 kb on + strand, within GFF6033 at 94.265 kb on + strand, within GFF6033 at 94.265 kb on + strand, within GFF6033 at 94.266 kb on - strand, within GFF6033 at 94.266 kb on - strand, within GFF6033 at 94.266 kb on - strand, within GFF6033 at 94.305 kb on + strand at 94.306 kb on - strand at 94.317 kb on + strand at 94.318 kb on - strand at 94.328 kb on + strand at 94.329 kb on - strand at 94.338 kb on + strand at 94.338 kb on + strand at 94.338 kb on + strand at 94.338 kb on + strand at 94.338 kb on + strand at 94.338 kb on + strand at 94.339 kb on - strand at 94.339 kb on - strand at 94.339 kb on - strand at 94.339 kb on - strand at 94.339 kb on - strand at 94.339 kb on - strand at 94.390 kb on + strand at 94.390 kb on + strand at 94.390 kb on + strand at 94.391 kb on - strand at 94.391 kb on - strand at 94.391 kb on - strand at 94.391 kb on - strand at 94.391 kb on - strand at 94.391 kb on - strand at 94.402 kb on + strand at 94.402 kb on + strand at 94.402 kb on + strand at 94.402 kb on + strand at 94.402 kb on + strand at 94.403 kb on - strand at 94.403 kb on - strand at 94.403 kb on - strand at 94.403 kb on - strand at 94.403 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 91,464 + +0.2 91,465 - +1.6 91,465 - -0.7 91,465 - +0.0 91,530 + GFF6031 0.11 +0.1 91,530 + GFF6031 0.11 +0.8 91,531 - GFF6031 0.11 +1.2 91,531 - GFF6031 0.11 -1.6 91,531 - GFF6031 0.11 +0.2 91,531 - GFF6031 0.11 +0.9 91,746 + GFF6031 0.39 -1.0 91,767 + GFF6031 0.42 +0.2 91,767 + GFF6031 0.42 +0.3 91,794 + GFF6031 0.45 +0.8 91,794 + GFF6031 0.45 +0.3 91,794 + GFF6031 0.45 +0.5 91,795 - GFF6031 0.45 +0.1 91,795 - GFF6031 0.45 +1.2 91,795 - GFF6031 0.45 -0.2 92,007 + GFF6031 0.72 -0.8 92,049 + GFF6031 0.78 -0.0 92,049 + GFF6031 0.78 -0.1 92,050 - GFF6031 0.78 -0.7 92,050 - GFF6031 0.78 -0.0 92,112 + GFF6031 0.86 -0.1 92,113 - GFF6031 0.86 +0.3 92,113 - GFF6031 0.86 +0.3 92,299 - +0.2 92,528 + GFF6032 0.24 +0.2 92,528 + GFF6032 0.24 +0.7 92,528 + GFF6032 0.24 +2.5 92,528 + GFF6032 0.24 -0.0 92,528 + GFF6032 0.24 -1.2 92,528 + GFF6032 0.24 -0.6 92,874 - GFF6032 0.52 -0.0 92,874 - GFF6032 0.52 -1.9 92,874 - GFF6032 0.52 +1.8 92,874 - GFF6032 0.52 -0.4 92,874 - GFF6032 0.52 -0.0 92,874 - GFF6032 0.52 -0.7 92,874 - GFF6032 0.52 +2.3 92,876 + GFF6032 0.52 +0.3 92,876 + GFF6032 0.52 -0.2 92,876 + GFF6032 0.52 +0.8 92,876 + GFF6032 0.52 +0.2 92,876 + GFF6032 0.52 -0.2 92,877 - GFF6032 0.53 +0.5 92,877 - GFF6032 0.53 +0.8 92,879 + GFF6032 0.53 +0.1 92,879 + GFF6032 0.53 +1.0 92,879 + GFF6032 0.53 -0.6 92,880 - GFF6032 0.53 +1.1 92,880 - GFF6032 0.53 +0.6 92,880 - GFF6032 0.53 -2.3 92,880 - GFF6032 0.53 +0.2 93,065 + GFF6032 0.68 -0.0 93,066 - GFF6032 0.68 +2.1 93,240 - GFF6032 0.83 +3.8 93,240 - GFF6032 0.83 +0.2 93,266 + GFF6032 0.85 -0.2 93,266 + GFF6032 0.85 +0.6 93,266 + GFF6032 0.85 +0.8 93,266 + GFF6032 0.85 -1.7 93,266 + GFF6032 0.85 +2.6 93,266 + GFF6032 0.85 +0.1 93,266 + GFF6032 0.85 +2.5 93,266 + GFF6032 0.85 -0.8 93,267 - GFF6032 0.85 +1.0 93,267 - GFF6032 0.85 +1.2 93,267 - GFF6032 0.85 +0.6 93,267 - GFF6032 0.85 -0.8 93,290 + GFF6032 0.87 +0.7 93,329 + GFF6032 0.90 +0.2 93,329 + GFF6032 0.90 -1.5 93,330 - +0.4 93,330 - -1.0 93,330 - -0.2 93,422 + +0.6 93,422 + -0.8 93,422 + -0.8 93,423 - -1.2 93,423 - +0.2 93,423 - +1.2 93,462 + -0.6 93,552 + -1.6 93,552 + +0.8 93,552 + +1.1 93,552 + -1.8 93,553 - +0.2 93,553 - -1.6 93,733 - GFF6033 0.29 +1.7 93,744 + GFF6033 0.30 +0.9 93,744 + GFF6033 0.30 +0.4 93,744 + GFF6033 0.30 -0.3 93,744 + GFF6033 0.30 +0.3 93,744 + GFF6033 0.30 +1.4 93,745 - GFF6033 0.31 -0.5 93,745 - GFF6033 0.31 +0.5 93,745 - GFF6033 0.31 -0.2 93,745 - GFF6033 0.31 +0.9 93,745 - GFF6033 0.31 +0.8 93,745 - GFF6033 0.31 +0.5 93,745 - GFF6033 0.31 -0.6 93,745 - GFF6033 0.31 +0.0 93,882 + GFF6033 0.45 +0.4 93,882 + GFF6033 0.45 -0.8 93,883 - GFF6033 0.45 -0.3 93,883 - GFF6033 0.45 +0.5 93,883 - GFF6033 0.45 +0.0 93,883 - GFF6033 0.45 -0.0 94,003 - GFF6033 0.58 +0.2 94,265 + GFF6033 0.86 -0.6 94,265 + GFF6033 0.86 -0.3 94,265 + GFF6033 0.86 +1.3 94,265 + GFF6033 0.86 +0.0 94,266 - GFF6033 0.87 +0.7 94,266 - GFF6033 0.87 -0.8 94,266 - GFF6033 0.87 +0.1 94,305 + -0.0 94,306 - -1.2 94,317 + +0.3 94,318 - -1.4 94,328 + -0.0 94,329 - +0.2 94,338 + +0.3 94,338 + +1.1 94,338 + +0.8 94,338 + -0.0 94,338 + -0.2 94,338 + -0.2 94,339 - -1.0 94,339 - -0.2 94,339 - -0.1 94,339 - -0.9 94,339 - -0.2 94,339 - +1.2 94,390 + -2.0 94,390 + -0.2 94,390 + +0.1 94,391 - -0.7 94,391 - -2.1 94,391 - +0.2 94,391 - -0.8 94,391 - -0.5 94,391 - +0.2 94,402 + -0.8 94,402 + +0.3 94,402 + +0.0 94,402 + +0.4 94,402 + -0.1 94,403 - +0.2 94,403 - -0.9 94,403 - +0.8 94,403 - -0.7 94,403 - +0.1
Or see this region's nucleotide sequence