Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6017 and GFF6018 overlap by 4 nucleotides GFF6018 and GFF6019 are separated by 7 nucleotides GFF6019 and GFF6020 overlap by 1 nucleotides GFF6020 and GFF6021 are separated by 104 nucleotides
GFF6017 - NADPH-dependent 7-cyano-7-deazaguanine reductase (EC 1.7.1.13), at 77,270 to 78,136
GFF6017
GFF6018 - Hydrolase, alpha/beta fold family functionally coupled to Phosphoribulokinase, at 78,133 to 79,035
GFF6018
GFF6019 - Similar to eukaryotic Peptidyl prolyl 4-hydroxylase, alpha subunit (EC 1.14.11.2), at 79,043 to 79,885
GFF6019
GFF6020 - UPF0246 protein YaaA, at 79,885 to 80,667
GFF6020
GFF6021 - Valyl-tRNA synthetase (EC 6.1.1.9), at 80,772 to 83,651
GFF6021
Position (kb)
79
80 Strain fitness (log2 ratio)
-2
-1
0
1 at 78.063 kb on + strand at 78.076 kb on - strand at 78.416 kb on + strand, within GFF6018 at 78.417 kb on - strand, within GFF6018 at 78.417 kb on - strand, within GFF6018 at 79.044 kb on + strand at 79.044 kb on + strand at 79.149 kb on + strand, within GFF6019 at 79.149 kb on + strand, within GFF6019 at 79.150 kb on - strand, within GFF6019 at 79.242 kb on + strand, within GFF6019 at 79.242 kb on + strand, within GFF6019 at 79.242 kb on + strand, within GFF6019 at 79.242 kb on + strand, within GFF6019 at 79.242 kb on + strand, within GFF6019 at 79.243 kb on - strand, within GFF6019 at 79.243 kb on - strand, within GFF6019 at 79.243 kb on - strand, within GFF6019 at 79.243 kb on - strand, within GFF6019 at 79.243 kb on - strand, within GFF6019 at 79.318 kb on - strand, within GFF6019 at 79.318 kb on - strand, within GFF6019 at 79.318 kb on - strand, within GFF6019 at 79.324 kb on - strand, within GFF6019 at 79.660 kb on - strand, within GFF6019 at 79.943 kb on + strand at 79.996 kb on + strand, within GFF6020 at 80.054 kb on + strand, within GFF6020 at 80.054 kb on + strand, within GFF6020 at 80.054 kb on + strand, within GFF6020 at 80.054 kb on + strand, within GFF6020 at 80.055 kb on - strand, within GFF6020 at 80.055 kb on - strand, within GFF6020 at 80.055 kb on - strand, within GFF6020 at 80.055 kb on - strand, within GFF6020 at 80.055 kb on - strand, within GFF6020 at 80.132 kb on + strand, within GFF6020 at 80.133 kb on - strand, within GFF6020 at 80.133 kb on - strand, within GFF6020 at 80.226 kb on - strand, within GFF6020 at 80.276 kb on + strand, within GFF6020 at 80.277 kb on - strand, within GFF6020 at 80.394 kb on - strand, within GFF6020 at 80.630 kb on + strand at 80.630 kb on + strand at 80.631 kb on - strand at 80.676 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 78,063 + +0.5 78,076 - +0.2 78,416 + GFF6018 0.31 -1.0 78,417 - GFF6018 0.31 -0.3 78,417 - GFF6018 0.31 -0.1 79,044 + +0.3 79,044 + -0.0 79,149 + GFF6019 0.13 -2.1 79,149 + GFF6019 0.13 -0.0 79,150 - GFF6019 0.13 -0.2 79,242 + GFF6019 0.24 -2.3 79,242 + GFF6019 0.24 -0.4 79,242 + GFF6019 0.24 -0.9 79,242 + GFF6019 0.24 -1.1 79,242 + GFF6019 0.24 -0.0 79,243 - GFF6019 0.24 -0.4 79,243 - GFF6019 0.24 -0.2 79,243 - GFF6019 0.24 -0.8 79,243 - GFF6019 0.24 +0.0 79,243 - GFF6019 0.24 -2.0 79,318 - GFF6019 0.33 +0.5 79,318 - GFF6019 0.33 -0.1 79,318 - GFF6019 0.33 -0.5 79,324 - GFF6019 0.33 -0.9 79,660 - GFF6019 0.73 -0.4 79,943 + -0.7 79,996 + GFF6020 0.14 +0.2 80,054 + GFF6020 0.22 +0.5 80,054 + GFF6020 0.22 +0.1 80,054 + GFF6020 0.22 -0.2 80,054 + GFF6020 0.22 -0.2 80,055 - GFF6020 0.22 -0.6 80,055 - GFF6020 0.22 -1.1 80,055 - GFF6020 0.22 -0.8 80,055 - GFF6020 0.22 +0.1 80,055 - GFF6020 0.22 -1.1 80,132 + GFF6020 0.32 +0.5 80,133 - GFF6020 0.32 +0.2 80,133 - GFF6020 0.32 -0.2 80,226 - GFF6020 0.44 -1.0 80,276 + GFF6020 0.50 -0.9 80,277 - GFF6020 0.50 -0.1 80,394 - GFF6020 0.65 -0.7 80,630 + -0.5 80,630 + +0.3 80,631 - -0.8 80,676 + +0.7
Or see this region's nucleotide sequence