Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6016 and GFF6017 are separated by 125 nucleotides GFF6017 and GFF6018 overlap by 4 nucleotides GFF6018 and GFF6019 are separated by 7 nucleotides
GFF6016 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 76,215 to 77,144
GFF6016
GFF6017 - NADPH-dependent 7-cyano-7-deazaguanine reductase (EC 1.7.1.13), at 77,270 to 78,136
GFF6017
GFF6018 - Hydrolase, alpha/beta fold family functionally coupled to Phosphoribulokinase, at 78,133 to 79,035
GFF6018
GFF6019 - Similar to eukaryotic Peptidyl prolyl 4-hydroxylase, alpha subunit (EC 1.14.11.2), at 79,043 to 79,885
GFF6019
Position (kb) 77
78
79 Strain fitness (log2 ratio) -1
0
1 at 76.432 kb on + strand, within GFF6016 at 76.433 kb on - strand, within GFF6016 at 76.636 kb on + strand, within GFF6016 at 76.637 kb on - strand, within GFF6016 at 76.637 kb on - strand, within GFF6016 at 76.687 kb on - strand, within GFF6016 at 76.687 kb on - strand, within GFF6016 at 76.741 kb on + strand, within GFF6016 at 76.742 kb on - strand, within GFF6016 at 76.742 kb on - strand, within GFF6016 at 76.742 kb on - strand, within GFF6016 at 76.742 kb on - strand, within GFF6016 at 76.744 kb on + strand, within GFF6016 at 76.744 kb on + strand, within GFF6016 at 76.744 kb on + strand, within GFF6016 at 76.745 kb on - strand, within GFF6016 at 76.753 kb on + strand, within GFF6016 at 77.358 kb on + strand, within GFF6017 at 77.359 kb on - strand, within GFF6017 at 77.359 kb on - strand, within GFF6017 at 77.367 kb on + strand, within GFF6017 at 77.367 kb on + strand, within GFF6017 at 77.368 kb on - strand, within GFF6017 at 77.368 kb on - strand, within GFF6017 at 77.469 kb on + strand, within GFF6017 at 77.469 kb on + strand, within GFF6017 at 77.469 kb on + strand, within GFF6017 at 77.470 kb on - strand, within GFF6017 at 77.470 kb on - strand, within GFF6017 at 77.470 kb on - strand, within GFF6017 at 77.508 kb on + strand, within GFF6017 at 77.640 kb on + strand, within GFF6017 at 77.851 kb on - strand, within GFF6017 at 78.063 kb on + strand at 78.076 kb on - strand at 78.416 kb on + strand, within GFF6018 at 78.417 kb on - strand, within GFF6018 at 78.417 kb on - strand, within GFF6018 at 79.044 kb on + strand at 79.044 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 76,432 + GFF6016 0.23 +0.5 76,433 - GFF6016 0.23 +0.2 76,636 + GFF6016 0.45 +0.3 76,637 - GFF6016 0.45 +0.2 76,637 - GFF6016 0.45 -0.2 76,687 - GFF6016 0.51 -1.0 76,687 - GFF6016 0.51 -0.6 76,741 + GFF6016 0.57 -0.8 76,742 - GFF6016 0.57 +1.4 76,742 - GFF6016 0.57 -0.2 76,742 - GFF6016 0.57 -1.7 76,742 - GFF6016 0.57 -1.0 76,744 + GFF6016 0.57 -1.6 76,744 + GFF6016 0.57 -1.4 76,744 + GFF6016 0.57 +0.5 76,745 - GFF6016 0.57 -0.4 76,753 + GFF6016 0.58 -0.9 77,358 + GFF6017 0.10 -0.6 77,359 - GFF6017 0.10 +1.4 77,359 - GFF6017 0.10 +0.9 77,367 + GFF6017 0.11 +0.0 77,367 + GFF6017 0.11 -0.5 77,368 - GFF6017 0.11 +0.3 77,368 - GFF6017 0.11 +0.1 77,469 + GFF6017 0.23 +1.2 77,469 + GFF6017 0.23 +0.6 77,469 + GFF6017 0.23 +0.8 77,470 - GFF6017 0.23 +0.3 77,470 - GFF6017 0.23 +0.8 77,470 - GFF6017 0.23 +1.0 77,508 + GFF6017 0.27 +0.8 77,640 + GFF6017 0.43 +0.2 77,851 - GFF6017 0.67 +1.5 78,063 + +0.5 78,076 - +0.2 78,416 + GFF6018 0.31 -1.0 78,417 - GFF6018 0.31 -0.3 78,417 - GFF6018 0.31 -0.1 79,044 + +0.3 79,044 + -0.0
Or see this region's nucleotide sequence