Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5845 and GFF5846 are separated by 87 nucleotides GFF5846 and GFF5847 are separated by 1 nucleotides GFF5847 and GFF5848 overlap by 1 nucleotides GFF5848 and GFF5849 are separated by 13 nucleotides
GFF5845 - NAD synthetase (EC 6.3.1.5), at 22,425 to 23,282
GFF5845
GFF5846 - FIG00453548: hypothetical protein, at 23,370 to 24,089
GFF5846
GFF5847 - putative membrane protein, at 24,091 to 26,745
GFF5847
GFF5848 - Flagellar motor protein, at 26,745 to 27,404
GFF5848
GFF5849 - FIG01210206: hypothetical protein, at 27,418 to 28,077
GFF5849
Position (kb) 24
25
26
27 Strain fitness (log2 ratio) -2
-1
0
1 at 23.145 kb on + strand, within GFF5845 at 23.145 kb on + strand, within GFF5845 at 23.145 kb on + strand, within GFF5845 at 23.145 kb on + strand, within GFF5845 at 23.145 kb on + strand, within GFF5845 at 23.146 kb on - strand, within GFF5845 at 23.146 kb on - strand, within GFF5845 at 23.146 kb on - strand, within GFF5845 at 23.146 kb on - strand, within GFF5845 at 23.146 kb on - strand, within GFF5845 at 23.340 kb on + strand at 23.340 kb on + strand at 23.340 kb on + strand at 23.341 kb on - strand at 23.348 kb on + strand at 23.349 kb on - strand at 24.160 kb on + strand at 24.161 kb on - strand at 24.214 kb on + strand at 24.215 kb on - strand at 24.409 kb on + strand, within GFF5847 at 24.409 kb on + strand, within GFF5847 at 24.409 kb on + strand, within GFF5847 at 24.410 kb on - strand, within GFF5847 at 24.410 kb on - strand, within GFF5847 at 24.410 kb on - strand, within GFF5847 at 24.871 kb on + strand, within GFF5847 at 24.872 kb on - strand, within GFF5847 at 24.872 kb on - strand, within GFF5847 at 24.872 kb on - strand, within GFF5847 at 24.872 kb on - strand, within GFF5847 at 25.148 kb on + strand, within GFF5847 at 25.148 kb on + strand, within GFF5847 at 25.508 kb on - strand, within GFF5847 at 25.648 kb on + strand, within GFF5847 at 25.648 kb on + strand, within GFF5847 at 25.649 kb on - strand, within GFF5847 at 26.632 kb on + strand at 26.632 kb on + strand at 26.632 kb on + strand at 26.632 kb on + strand at 26.632 kb on + strand at 26.632 kb on + strand at 26.633 kb on - strand at 26.633 kb on - strand at 26.633 kb on - strand at 26.633 kb on - strand at 26.633 kb on - strand at 26.633 kb on - strand at 27.099 kb on + strand, within GFF5848 at 27.099 kb on + strand, within GFF5848 at 27.099 kb on + strand, within GFF5848 at 27.100 kb on - strand, within GFF5848 at 27.100 kb on - strand, within GFF5848 at 27.100 kb on - strand, within GFF5848 at 27.583 kb on + strand, within GFF5849
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 23,145 + GFF5845 0.84 +0.9 23,145 + GFF5845 0.84 +0.9 23,145 + GFF5845 0.84 +0.0 23,145 + GFF5845 0.84 -0.4 23,145 + GFF5845 0.84 -1.6 23,146 - GFF5845 0.84 -0.1 23,146 - GFF5845 0.84 -1.7 23,146 - GFF5845 0.84 +0.2 23,146 - GFF5845 0.84 -0.5 23,146 - GFF5845 0.84 -1.4 23,340 + -0.4 23,340 + -0.3 23,340 + -0.4 23,341 - -0.1 23,348 + -0.2 23,349 - -0.5 24,160 + +0.4 24,161 - -0.3 24,214 + +0.2 24,215 - -0.1 24,409 + GFF5847 0.12 -0.3 24,409 + GFF5847 0.12 -0.6 24,409 + GFF5847 0.12 +0.9 24,410 - GFF5847 0.12 +0.6 24,410 - GFF5847 0.12 +0.3 24,410 - GFF5847 0.12 -0.7 24,871 + GFF5847 0.29 -0.6 24,872 - GFF5847 0.29 +0.3 24,872 - GFF5847 0.29 -0.4 24,872 - GFF5847 0.29 -0.0 24,872 - GFF5847 0.29 +0.2 25,148 + GFF5847 0.40 -0.4 25,148 + GFF5847 0.40 -0.7 25,508 - GFF5847 0.53 +0.1 25,648 + GFF5847 0.59 +0.1 25,648 + GFF5847 0.59 +0.8 25,649 - GFF5847 0.59 -0.4 26,632 + +0.6 26,632 + -0.5 26,632 + -0.6 26,632 + -0.4 26,632 + -2.1 26,632 + -0.7 26,633 - +1.3 26,633 - +0.5 26,633 - -0.8 26,633 - -0.3 26,633 - -1.5 26,633 - +0.5 27,099 + GFF5848 0.54 -0.7 27,099 + GFF5848 0.54 +0.2 27,099 + GFF5848 0.54 -0.1 27,100 - GFF5848 0.54 -0.7 27,100 - GFF5848 0.54 -0.4 27,100 - GFF5848 0.54 -0.6 27,583 + GFF5849 0.25 +0.5
Or see this region's nucleotide sequence