Strain Fitness in Variovorax sp. SCN45 around GFF58

Experiment: M9 with calcium pantothenate 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF57 and GFF58 are separated by 93 nucleotidesGFF58 and GFF59 overlap by 4 nucleotidesGFF59 and GFF60 are separated by 66 nucleotides GFF57 - Putative polysaccharide export protein YccZ precursor, at 71,661 to 72,872 GFF57 GFF58 - Tyrosine-protein kinase (EC 2.7.10.2), at 72,966 to 75,230 GFF58 GFF59 - Undecaprenyl-phosphate galactosephosphotransferase (EC 2.7.8.6), at 75,227 to 75,928 GFF59 GFF60 - GDP-L-fucose synthetase (EC 1.1.1.271), at 75,995 to 76,942 GFF60 Position (kb) 72 73 74 75 76Strain fitness (log2 ratio) -2 -1 0 1at 71.983 kb on - strand, within GFF57at 71.983 kb on - strand, within GFF57at 72.022 kb on - strand, within GFF57at 72.022 kb on - strand, within GFF57at 72.022 kb on - strand, within GFF57at 72.141 kb on + strand, within GFF57at 72.274 kb on - strand, within GFF57at 72.525 kb on + strand, within GFF57at 72.526 kb on - strand, within GFF57at 72.526 kb on - strand, within GFF57at 72.651 kb on + strand, within GFF57at 72.651 kb on + strand, within GFF57at 72.651 kb on + strand, within GFF57at 72.651 kb on + strand, within GFF57at 72.652 kb on - strand, within GFF57at 72.652 kb on - strand, within GFF57at 72.652 kb on - strand, within GFF57at 72.652 kb on - strand, within GFF57at 72.652 kb on - strand, within GFF57at 72.771 kb on + strandat 72.771 kb on + strandat 72.771 kb on + strandat 72.771 kb on + strandat 72.772 kb on - strandat 72.772 kb on - strandat 73.084 kb on - strandat 73.278 kb on + strand, within GFF58at 73.278 kb on + strand, within GFF58at 73.279 kb on - strand, within GFF58at 73.299 kb on + strand, within GFF58at 73.299 kb on + strand, within GFF58at 73.299 kb on + strand, within GFF58at 73.299 kb on + strand, within GFF58at 73.299 kb on + strand, within GFF58at 73.299 kb on + strand, within GFF58at 73.300 kb on - strand, within GFF58at 73.300 kb on - strand, within GFF58at 73.300 kb on - strand, within GFF58at 73.300 kb on - strand, within GFF58at 73.300 kb on - strand, within GFF58at 73.378 kb on - strand, within GFF58at 73.476 kb on + strand, within GFF58at 73.476 kb on + strand, within GFF58at 73.477 kb on - strand, within GFF58at 73.477 kb on - strand, within GFF58at 73.620 kb on + strand, within GFF58at 73.621 kb on - strand, within GFF58at 73.621 kb on - strand, within GFF58at 73.764 kb on + strand, within GFF58at 73.764 kb on + strand, within GFF58at 73.764 kb on + strand, within GFF58at 73.765 kb on - strand, within GFF58at 73.765 kb on - strand, within GFF58at 73.765 kb on - strand, within GFF58at 73.875 kb on + strand, within GFF58at 73.875 kb on + strand, within GFF58at 73.875 kb on + strand, within GFF58at 73.875 kb on + strand, within GFF58at 73.875 kb on + strand, within GFF58at 73.875 kb on + strand, within GFF58at 73.875 kb on + strand, within GFF58at 73.875 kb on + strand, within GFF58at 73.875 kb on + strand, within GFF58at 73.876 kb on - strand, within GFF58at 73.876 kb on - strand, within GFF58at 73.876 kb on - strand, within GFF58at 73.876 kb on - strand, within GFF58at 73.885 kb on - strand, within GFF58at 74.146 kb on - strand, within GFF58at 74.409 kb on + strand, within GFF58at 74.409 kb on + strand, within GFF58at 74.410 kb on - strand, within GFF58at 74.410 kb on - strand, within GFF58at 74.791 kb on - strand, within GFF58at 74.934 kb on - strand, within GFF58at 75.121 kb on - strandat 75.127 kb on - strandat 75.136 kb on - strandat 75.294 kb on - strandat 75.390 kb on - strand, within GFF59at 75.399 kb on + strand, within GFF59at 75.440 kb on + strand, within GFF59at 75.441 kb on - strand, within GFF59at 75.441 kb on - strand, within GFF59at 75.441 kb on - strand, within GFF59at 75.441 kb on - strand, within GFF59at 75.441 kb on - strand, within GFF59at 75.441 kb on - strand, within GFF59at 75.441 kb on - strand, within GFF59at 75.441 kb on - strand, within GFF59at 75.575 kb on + strand, within GFF59at 75.576 kb on - strand, within GFF59at 75.632 kb on + strand, within GFF59at 75.633 kb on - strand, within GFF59at 75.633 kb on - strand, within GFF59at 75.633 kb on - strand, within GFF59at 75.633 kb on - strand, within GFF59at 75.728 kb on + strand, within GFF59at 75.737 kb on + strand, within GFF59at 75.738 kb on - strand, within GFF59at 75.738 kb on - strand, within GFF59at 75.750 kb on - strand, within GFF59at 75.812 kb on + strand, within GFF59at 75.813 kb on - strand, within GFF59at 75.843 kb on - strand, within GFF59at 75.843 kb on - strand, within GFF59at 75.965 kb on - strandat 76.130 kb on + strand, within GFF60at 76.130 kb on + strand, within GFF60at 76.130 kb on + strand, within GFF60at 76.131 kb on - strand, within GFF60at 76.131 kb on - strand, within GFF60at 76.175 kb on + strand, within GFF60at 76.176 kb on - strand, within GFF60at 76.193 kb on + strand, within GFF60at 76.194 kb on - strand, within GFF60

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL
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71,983 - GFF57 0.27 -1.8
71,983 - GFF57 0.27 +0.4
72,022 - GFF57 0.30 -1.2
72,022 - GFF57 0.30 +0.0
72,022 - GFF57 0.30 -0.7
72,141 + GFF57 0.40 -0.1
72,274 - GFF57 0.51 -1.4
72,525 + GFF57 0.71 -1.3
72,526 - GFF57 0.71 +0.0
72,526 - GFF57 0.71 +1.3
72,651 + GFF57 0.82 -0.2
72,651 + GFF57 0.82 +1.1
72,651 + GFF57 0.82 -1.4
72,651 + GFF57 0.82 -1.3
72,652 - GFF57 0.82 +0.7
72,652 - GFF57 0.82 +0.1
72,652 - GFF57 0.82 +0.1
72,652 - GFF57 0.82 -0.5
72,652 - GFF57 0.82 +0.4
72,771 + +0.4
72,771 + -0.3
72,771 + +0.2
72,771 + +0.7
72,772 - +0.3
72,772 - -0.0
73,084 - -0.8
73,278 + GFF58 0.14 +1.0
73,278 + GFF58 0.14 -0.9
73,279 - GFF58 0.14 -0.1
73,299 + GFF58 0.15 -0.9
73,299 + GFF58 0.15 -0.8
73,299 + GFF58 0.15 -0.1
73,299 + GFF58 0.15 +0.2
73,299 + GFF58 0.15 -0.2
73,299 + GFF58 0.15 -0.2
73,300 - GFF58 0.15 -0.2
73,300 - GFF58 0.15 +1.2
73,300 - GFF58 0.15 +0.3
73,300 - GFF58 0.15 +0.9
73,300 - GFF58 0.15 +1.4
73,378 - GFF58 0.18 +0.4
73,476 + GFF58 0.23 +0.6
73,476 + GFF58 0.23 +0.6
73,477 - GFF58 0.23 +0.4
73,477 - GFF58 0.23 +0.3
73,620 + GFF58 0.29 +0.1
73,621 - GFF58 0.29 +0.7
73,621 - GFF58 0.29 -0.1
73,764 + GFF58 0.35 -0.3
73,764 + GFF58 0.35 -0.8
73,764 + GFF58 0.35 -1.3
73,765 - GFF58 0.35 +0.9
73,765 - GFF58 0.35 -0.6
73,765 - GFF58 0.35 -1.8
73,875 + GFF58 0.40 -0.4
73,875 + GFF58 0.40 +0.8
73,875 + GFF58 0.40 -0.6
73,875 + GFF58 0.40 -1.5
73,875 + GFF58 0.40 -0.0
73,875 + GFF58 0.40 +0.5
73,875 + GFF58 0.40 +0.9
73,875 + GFF58 0.40 -0.2
73,875 + GFF58 0.40 +0.5
73,876 - GFF58 0.40 -0.0
73,876 - GFF58 0.40 +0.4
73,876 - GFF58 0.40 +0.4
73,876 - GFF58 0.40 -0.6
73,885 - GFF58 0.41 +0.3
74,146 - GFF58 0.52 -0.6
74,409 + GFF58 0.64 +1.0
74,409 + GFF58 0.64 +0.6
74,410 - GFF58 0.64 +0.2
74,410 - GFF58 0.64 -0.2
74,791 - GFF58 0.81 -0.1
74,934 - GFF58 0.87 -0.4
75,121 - -1.1
75,127 - -1.2
75,136 - -0.8
75,294 - -0.1
75,390 - GFF59 0.23 -0.5
75,399 + GFF59 0.25 -0.8
75,440 + GFF59 0.30 -0.9
75,441 - GFF59 0.30 +0.9
75,441 - GFF59 0.30 +0.1
75,441 - GFF59 0.30 +0.3
75,441 - GFF59 0.30 -0.5
75,441 - GFF59 0.30 -0.3
75,441 - GFF59 0.30 -0.5
75,441 - GFF59 0.30 -0.1
75,441 - GFF59 0.30 -1.2
75,575 + GFF59 0.50 -1.0
75,576 - GFF59 0.50 +0.0
75,632 + GFF59 0.58 -0.8
75,633 - GFF59 0.58 -1.7
75,633 - GFF59 0.58 -0.2
75,633 - GFF59 0.58 +0.4
75,633 - GFF59 0.58 +0.3
75,728 + GFF59 0.71 -0.1
75,737 + GFF59 0.73 -0.4
75,738 - GFF59 0.73 +0.4
75,738 - GFF59 0.73 +1.0
75,750 - GFF59 0.75 -0.2
75,812 + GFF59 0.83 +1.4
75,813 - GFF59 0.83 +0.3
75,843 - GFF59 0.88 +0.2
75,843 - GFF59 0.88 -0.0
75,965 - -0.6
76,130 + GFF60 0.14 -0.2
76,130 + GFF60 0.14 -0.9
76,130 + GFF60 0.14 -1.1
76,131 - GFF60 0.14 -0.4
76,131 - GFF60 0.14 +0.2
76,175 + GFF60 0.19 -0.0
76,176 - GFF60 0.19 -1.1
76,193 + GFF60 0.21 -0.2
76,194 - GFF60 0.21 -0.3

Or see this region's nucleotide sequence