Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5373 and GFF5374 are separated by 86 nucleotides
GFF5373 - Xylose isomerase-like TIM-barrel protein KPN_00539, at 45 to 452
GFF5373
GFF5374 - Glutamine amidotransferase, class I, at 539 to 1,483
GFF5374
Position (kb)
0
1 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 0.046 kb on + strand at 0.046 kb on + strand at 0.046 kb on + strand at 0.046 kb on + strand at 0.088 kb on + strand, within GFF5373 at 0.088 kb on + strand, within GFF5373 at 0.088 kb on + strand, within GFF5373 at 0.175 kb on + strand, within GFF5373 at 0.175 kb on + strand, within GFF5373 at 0.175 kb on + strand, within GFF5373 at 0.176 kb on - strand, within GFF5373 at 0.176 kb on - strand, within GFF5373 at 0.176 kb on - strand, within GFF5373 at 0.274 kb on + strand, within GFF5373 at 0.274 kb on + strand, within GFF5373 at 0.274 kb on + strand, within GFF5373 at 0.274 kb on + strand, within GFF5373 at 0.274 kb on + strand, within GFF5373 at 0.274 kb on + strand, within GFF5373 at 0.275 kb on - strand, within GFF5373 at 0.275 kb on - strand, within GFF5373 at 0.275 kb on - strand, within GFF5373 at 0.275 kb on - strand, within GFF5373 at 0.275 kb on - strand, within GFF5373 at 0.275 kb on - strand, within GFF5373 at 0.275 kb on - strand, within GFF5373 at 0.275 kb on - strand, within GFF5373 at 0.275 kb on - strand, within GFF5373 at 0.275 kb on - strand, within GFF5373 at 0.275 kb on - strand, within GFF5373 at 0.346 kb on + strand, within GFF5373 at 0.346 kb on + strand, within GFF5373 at 0.346 kb on + strand, within GFF5373 at 0.346 kb on + strand, within GFF5373 at 0.346 kb on + strand, within GFF5373 at 0.346 kb on + strand, within GFF5373 at 0.346 kb on + strand, within GFF5373 at 0.347 kb on - strand, within GFF5373 at 0.347 kb on - strand, within GFF5373 at 0.347 kb on - strand, within GFF5373 at 0.347 kb on - strand, within GFF5373 at 0.347 kb on - strand, within GFF5373 at 0.493 kb on + strand at 0.493 kb on + strand at 0.493 kb on + strand at 0.493 kb on + strand at 0.493 kb on + strand at 0.493 kb on + strand at 0.493 kb on + strand at 0.493 kb on + strand at 0.493 kb on + strand at 0.494 kb on - strand at 0.494 kb on - strand at 0.494 kb on - strand at 0.494 kb on - strand at 0.494 kb on - strand at 0.494 kb on - strand at 0.494 kb on - strand at 0.692 kb on + strand, within GFF5374 at 0.692 kb on + strand, within GFF5374 at 0.692 kb on + strand, within GFF5374 at 0.692 kb on + strand, within GFF5374 at 0.692 kb on + strand, within GFF5374 at 0.692 kb on + strand, within GFF5374 at 0.692 kb on + strand, within GFF5374 at 0.692 kb on + strand, within GFF5374 at 0.692 kb on + strand, within GFF5374 at 0.693 kb on - strand, within GFF5374 at 0.693 kb on - strand, within GFF5374 at 0.693 kb on - strand, within GFF5374 at 0.693 kb on - strand, within GFF5374 at 0.800 kb on + strand, within GFF5374 at 0.800 kb on + strand, within GFF5374 at 0.801 kb on - strand, within GFF5374 at 0.801 kb on - strand, within GFF5374 at 0.860 kb on + strand, within GFF5374 at 0.860 kb on + strand, within GFF5374 at 0.860 kb on + strand, within GFF5374 at 0.860 kb on + strand, within GFF5374 at 0.861 kb on - strand, within GFF5374 at 0.861 kb on - strand, within GFF5374 at 0.861 kb on - strand, within GFF5374 at 0.861 kb on - strand, within GFF5374 at 0.861 kb on - strand, within GFF5374 at 0.861 kb on - strand, within GFF5374 at 0.861 kb on - strand, within GFF5374 at 0.914 kb on + strand, within GFF5374 at 0.914 kb on + strand, within GFF5374 at 0.915 kb on - strand, within GFF5374 at 0.915 kb on - strand, within GFF5374 at 0.915 kb on - strand, within GFF5374 at 0.915 kb on - strand, within GFF5374 at 0.915 kb on - strand, within GFF5374 at 1.016 kb on + strand, within GFF5374 at 1.016 kb on + strand, within GFF5374 at 1.016 kb on + strand, within GFF5374 at 1.016 kb on + strand, within GFF5374 at 1.016 kb on + strand, within GFF5374 at 1.016 kb on + strand, within GFF5374 at 1.017 kb on - strand, within GFF5374 at 1.017 kb on - strand, within GFF5374 at 1.079 kb on + strand, within GFF5374 at 1.079 kb on + strand, within GFF5374 at 1.079 kb on + strand, within GFF5374 at 1.079 kb on + strand, within GFF5374 at 1.079 kb on + strand, within GFF5374 at 1.079 kb on + strand, within GFF5374 at 1.079 kb on + strand, within GFF5374 at 1.079 kb on + strand, within GFF5374 at 1.080 kb on - strand, within GFF5374 at 1.080 kb on - strand, within GFF5374 at 1.080 kb on - strand, within GFF5374 at 1.080 kb on - strand, within GFF5374 at 1.080 kb on - strand, within GFF5374 at 1.080 kb on - strand, within GFF5374 at 1.137 kb on - strand, within GFF5374 at 1.137 kb on - strand, within GFF5374 at 1.137 kb on - strand, within GFF5374 at 1.137 kb on - strand, within GFF5374 at 1.280 kb on + strand, within GFF5374 at 1.280 kb on + strand, within GFF5374 at 1.280 kb on + strand, within GFF5374 at 1.280 kb on + strand, within GFF5374 at 1.280 kb on + strand, within GFF5374 at 1.280 kb on + strand, within GFF5374 at 1.280 kb on + strand, within GFF5374 at 1.280 kb on + strand, within GFF5374 at 1.280 kb on + strand, within GFF5374 at 1.280 kb on + strand, within GFF5374 at 1.281 kb on - strand, within GFF5374 at 1.281 kb on - strand, within GFF5374 at 1.281 kb on - strand, within GFF5374 at 1.281 kb on - strand, within GFF5374 at 1.281 kb on - strand, within GFF5374 at 1.319 kb on + strand, within GFF5374 at 1.319 kb on + strand, within GFF5374 at 1.319 kb on + strand, within GFF5374 at 1.319 kb on + strand, within GFF5374 at 1.319 kb on + strand, within GFF5374 at 1.320 kb on - strand, within GFF5374 at 1.320 kb on - strand, within GFF5374 at 1.320 kb on - strand, within GFF5374 at 1.320 kb on - strand, within GFF5374 at 1.320 kb on - strand, within GFF5374
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 46 + -0.1 46 + +0.0 46 + +0.7 46 + -1.8 88 + GFF5373 0.11 +0.2 88 + GFF5373 0.11 +0.1 88 + GFF5373 0.11 +0.2 175 + GFF5373 0.32 +0.5 175 + GFF5373 0.32 -2.1 175 + GFF5373 0.32 -0.1 176 - GFF5373 0.32 +0.0 176 - GFF5373 0.32 -0.3 176 - GFF5373 0.32 +0.1 274 + GFF5373 0.56 -0.5 274 + GFF5373 0.56 -0.8 274 + GFF5373 0.56 +0.6 274 + GFF5373 0.56 -0.2 274 + GFF5373 0.56 -1.8 274 + GFF5373 0.56 +0.1 275 - GFF5373 0.56 -0.6 275 - GFF5373 0.56 -0.1 275 - GFF5373 0.56 -0.9 275 - GFF5373 0.56 +0.0 275 - GFF5373 0.56 +1.1 275 - GFF5373 0.56 +2.0 275 - GFF5373 0.56 -0.8 275 - GFF5373 0.56 +0.3 275 - GFF5373 0.56 +0.9 275 - GFF5373 0.56 -1.5 275 - GFF5373 0.56 +0.5 346 + GFF5373 0.74 +0.9 346 + GFF5373 0.74 -0.6 346 + GFF5373 0.74 +0.8 346 + GFF5373 0.74 -0.6 346 + GFF5373 0.74 -0.1 346 + GFF5373 0.74 +0.3 346 + GFF5373 0.74 -0.1 347 - GFF5373 0.74 -0.3 347 - GFF5373 0.74 -0.1 347 - GFF5373 0.74 +2.0 347 - GFF5373 0.74 +0.4 347 - GFF5373 0.74 +0.5 493 + -0.4 493 + +0.8 493 + -1.3 493 + +0.9 493 + -0.8 493 + -0.2 493 + -0.3 493 + +0.8 493 + +0.3 494 - +1.2 494 - -0.4 494 - -2.4 494 - -1.2 494 - +0.5 494 - -0.9 494 - +0.4 692 + GFF5374 0.16 -1.8 692 + GFF5374 0.16 -0.2 692 + GFF5374 0.16 -0.3 692 + GFF5374 0.16 -2.2 692 + GFF5374 0.16 -1.0 692 + GFF5374 0.16 +1.0 692 + GFF5374 0.16 +2.0 692 + GFF5374 0.16 +1.3 692 + GFF5374 0.16 -0.4 693 - GFF5374 0.16 +0.5 693 - GFF5374 0.16 +0.3 693 - GFF5374 0.16 -1.4 693 - GFF5374 0.16 -1.0 800 + GFF5374 0.28 +0.1 800 + GFF5374 0.28 +0.2 801 - GFF5374 0.28 +0.7 801 - GFF5374 0.28 +0.3 860 + GFF5374 0.34 +1.2 860 + GFF5374 0.34 -0.4 860 + GFF5374 0.34 -0.4 860 + GFF5374 0.34 +2.4 861 - GFF5374 0.34 -0.5 861 - GFF5374 0.34 +1.0 861 - GFF5374 0.34 -1.2 861 - GFF5374 0.34 +0.2 861 - GFF5374 0.34 +0.5 861 - GFF5374 0.34 -0.4 861 - GFF5374 0.34 -0.6 914 + GFF5374 0.40 -1.0 914 + GFF5374 0.40 -0.7 915 - GFF5374 0.40 +0.2 915 - GFF5374 0.40 -0.5 915 - GFF5374 0.40 -0.1 915 - GFF5374 0.40 -0.9 915 - GFF5374 0.40 -0.9 1,016 + GFF5374 0.50 +0.8 1,016 + GFF5374 0.50 +0.7 1,016 + GFF5374 0.50 -0.1 1,016 + GFF5374 0.50 -0.5 1,016 + GFF5374 0.50 -2.5 1,016 + GFF5374 0.50 +1.1 1,017 - GFF5374 0.51 -0.1 1,017 - GFF5374 0.51 -0.4 1,079 + GFF5374 0.57 -0.6 1,079 + GFF5374 0.57 +1.0 1,079 + GFF5374 0.57 -0.9 1,079 + GFF5374 0.57 -0.7 1,079 + GFF5374 0.57 -0.3 1,079 + GFF5374 0.57 +0.4 1,079 + GFF5374 0.57 +0.2 1,079 + GFF5374 0.57 +0.7 1,080 - GFF5374 0.57 +0.2 1,080 - GFF5374 0.57 +0.1 1,080 - GFF5374 0.57 -0.3 1,080 - GFF5374 0.57 -1.6 1,080 - GFF5374 0.57 +0.1 1,080 - GFF5374 0.57 +1.4 1,137 - GFF5374 0.63 -0.3 1,137 - GFF5374 0.63 -0.8 1,137 - GFF5374 0.63 +0.3 1,137 - GFF5374 0.63 -0.1 1,280 + GFF5374 0.78 -0.3 1,280 + GFF5374 0.78 -0.3 1,280 + GFF5374 0.78 -0.0 1,280 + GFF5374 0.78 -0.3 1,280 + GFF5374 0.78 -0.3 1,280 + GFF5374 0.78 -0.3 1,280 + GFF5374 0.78 +1.0 1,280 + GFF5374 0.78 -0.2 1,280 + GFF5374 0.78 +1.1 1,280 + GFF5374 0.78 +2.0 1,281 - GFF5374 0.79 -0.6 1,281 - GFF5374 0.79 -0.1 1,281 - GFF5374 0.79 -0.6 1,281 - GFF5374 0.79 -0.2 1,281 - GFF5374 0.79 -0.3 1,319 + GFF5374 0.83 -0.2 1,319 + GFF5374 0.83 -1.6 1,319 + GFF5374 0.83 -0.3 1,319 + GFF5374 0.83 -1.1 1,319 + GFF5374 0.83 -1.0 1,320 - GFF5374 0.83 -0.3 1,320 - GFF5374 0.83 -0.1 1,320 - GFF5374 0.83 -0.6 1,320 - GFF5374 0.83 -0.2 1,320 - GFF5374 0.83 -1.0
Or see this region's nucleotide sequence