Strain Fitness in Variovorax sp. SCN45 around GFF5361

Experiment: M9 with calcium pantothenate 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5358 and GFF5359 overlap by 4 nucleotidesGFF5359 and GFF5360 overlap by 4 nucleotidesGFF5360 and GFF5361 are separated by 2 nucleotidesGFF5361 and GFF5362 are separated by 65 nucleotidesGFF5362 and GFF5363 are separated by 13 nucleotidesGFF5363 and GFF5364 are separated by 66 nucleotides GFF5358 - Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19), at 234,375 to 234,764 GFF5358 GFF5359 - Phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31), at 234,761 to 235,162 GFF5359 GFF5360 - Putative transmembrane protein, at 235,159 to 235,530 GFF5360 GFF5361 - FIG146285: Diadenosine tetraphosphate (Ap4A) hydrolase and other HIT family hydrolases, at 235,533 to 235,892 GFF5361 GFF5362 - Twin-arginine translocation protein TatA, at 235,958 to 236,203 GFF5362 GFF5363 - Twin-arginine translocation protein TatB, at 236,217 to 236,687 GFF5363 GFF5364 - Twin-arginine translocation protein TatC, at 236,754 to 237,566 GFF5364 Position (kb) 235 236Strain fitness (log2 ratio) -2 -1 0 1 2at 234.756 kb on + strandat 234.756 kb on + strandat 234.756 kb on + strandat 234.756 kb on + strandat 234.756 kb on + strandat 234.756 kb on + strandat 234.756 kb on + strandat 235.246 kb on + strand, within GFF5360at 235.246 kb on + strand, within GFF5360at 235.246 kb on + strand, within GFF5360at 235.247 kb on - strand, within GFF5360at 235.247 kb on - strand, within GFF5360at 235.247 kb on - strand, within GFF5360at 235.247 kb on - strand, within GFF5360at 235.247 kb on - strand, within GFF5360at 235.247 kb on - strand, within GFF5360at 235.247 kb on - strand, within GFF5360at 235.255 kb on + strand, within GFF5360at 235.255 kb on + strand, within GFF5360at 235.255 kb on + strand, within GFF5360at 235.255 kb on + strand, within GFF5360at 235.255 kb on + strand, within GFF5360at 235.256 kb on - strand, within GFF5360at 235.256 kb on - strand, within GFF5360at 235.256 kb on - strand, within GFF5360at 235.256 kb on - strand, within GFF5360at 235.256 kb on - strand, within GFF5360at 235.256 kb on - strand, within GFF5360at 235.312 kb on + strand, within GFF5360at 235.312 kb on + strand, within GFF5360at 235.312 kb on + strand, within GFF5360at 235.312 kb on + strand, within GFF5360at 235.312 kb on + strand, within GFF5360at 235.312 kb on + strand, within GFF5360at 235.312 kb on + strand, within GFF5360at 235.312 kb on + strand, within GFF5360at 235.312 kb on + strand, within GFF5360at 235.312 kb on + strand, within GFF5360at 235.312 kb on + strand, within GFF5360at 235.313 kb on - strand, within GFF5360at 235.313 kb on - strand, within GFF5360at 235.313 kb on - strand, within GFF5360at 235.313 kb on - strand, within GFF5360at 235.313 kb on - strand, within GFF5360at 235.313 kb on - strand, within GFF5360at 235.313 kb on - strand, within GFF5360at 235.313 kb on - strand, within GFF5360at 235.345 kb on + strand, within GFF5360at 235.346 kb on - strand, within GFF5360at 235.346 kb on - strand, within GFF5360at 235.474 kb on + strand, within GFF5360at 235.474 kb on + strand, within GFF5360at 235.475 kb on - strand, within GFF5360at 235.475 kb on - strand, within GFF5360at 235.475 kb on - strand, within GFF5360at 235.600 kb on - strand, within GFF5361at 235.900 kb on + strandat 235.918 kb on + strandat 235.918 kb on + strandat 235.918 kb on + strandat 235.918 kb on + strandat 236.541 kb on + strand, within GFF5363at 236.595 kb on + strand, within GFF5363at 236.685 kb on + strandat 236.685 kb on + strandat 236.685 kb on + strandat 236.685 kb on + strandat 236.685 kb on + strandat 236.686 kb on - strandat 236.686 kb on - strandat 236.686 kb on - strandat 236.686 kb on - strandat 236.686 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL
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234,756 + -0.2
234,756 + +0.8
234,756 + -0.2
234,756 + -0.1
234,756 + -0.9
234,756 + +0.4
234,756 + -0.1
235,246 + GFF5360 0.23 -0.3
235,246 + GFF5360 0.23 -1.5
235,246 + GFF5360 0.23 +0.4
235,247 - GFF5360 0.24 -1.3
235,247 - GFF5360 0.24 +0.3
235,247 - GFF5360 0.24 +1.0
235,247 - GFF5360 0.24 -0.7
235,247 - GFF5360 0.24 +0.2
235,247 - GFF5360 0.24 +0.6
235,247 - GFF5360 0.24 -0.5
235,255 + GFF5360 0.26 -0.6
235,255 + GFF5360 0.26 -0.0
235,255 + GFF5360 0.26 +0.5
235,255 + GFF5360 0.26 -0.0
235,255 + GFF5360 0.26 +0.1
235,256 - GFF5360 0.26 -0.4
235,256 - GFF5360 0.26 +0.1
235,256 - GFF5360 0.26 +0.8
235,256 - GFF5360 0.26 -0.6
235,256 - GFF5360 0.26 -0.5
235,256 - GFF5360 0.26 +0.2
235,312 + GFF5360 0.41 -0.8
235,312 + GFF5360 0.41 +0.8
235,312 + GFF5360 0.41 +0.1
235,312 + GFF5360 0.41 -0.1
235,312 + GFF5360 0.41 +1.0
235,312 + GFF5360 0.41 -1.3
235,312 + GFF5360 0.41 -1.0
235,312 + GFF5360 0.41 +0.2
235,312 + GFF5360 0.41 -0.9
235,312 + GFF5360 0.41 -2.0
235,312 + GFF5360 0.41 -0.2
235,313 - GFF5360 0.41 +0.3
235,313 - GFF5360 0.41 -0.4
235,313 - GFF5360 0.41 -0.4
235,313 - GFF5360 0.41 -0.1
235,313 - GFF5360 0.41 +0.1
235,313 - GFF5360 0.41 -1.0
235,313 - GFF5360 0.41 +0.2
235,313 - GFF5360 0.41 -0.3
235,345 + GFF5360 0.50 -0.7
235,346 - GFF5360 0.50 +1.8
235,346 - GFF5360 0.50 +1.1
235,474 + GFF5360 0.85 +0.2
235,474 + GFF5360 0.85 -0.2
235,475 - GFF5360 0.85 -0.5
235,475 - GFF5360 0.85 -0.5
235,475 - GFF5360 0.85 +0.2
235,600 - GFF5361 0.19 +0.5
235,900 + -0.8
235,918 + +0.2
235,918 + +0.2
235,918 + +1.2
235,918 + +0.8
236,541 + GFF5363 0.69 +1.2
236,595 + GFF5363 0.80 -1.0
236,685 + -0.2
236,685 + +0.6
236,685 + -0.3
236,685 + -0.2
236,685 + -1.2
236,686 - -0.1
236,686 - +0.6
236,686 - -0.9
236,686 - +0.4
236,686 - +0.8

Or see this region's nucleotide sequence