Strain Fitness in Variovorax sp. SCN45 around GFF53

Experiment: M9 with calcium pantothenate 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF52 and GFF53 overlap by 4 nucleotidesGFF53 and GFF54 are separated by 26 nucleotidesGFF54 and GFF55 overlap by 4 nucleotides GFF52 - Polyketide synthase modules and related proteins, at 56,854 to 64,083 GFF52 GFF53 - Polyketide synthase modules and related proteins, at 64,080 to 69,116 GFF53 GFF54 - 4'-phosphopantetheinyl transferase (EC 2.7.8.-), at 69,143 to 69,856 GFF54 GFF55 - hypothetical protein, at 69,853 to 70,671 GFF55 Position (kb) 64 65 66 67 68 69 70Strain fitness (log2 ratio) -2 -1 0 1 2at 63.172 kb on + strand, within GFF52at 63.412 kb on + strandat 63.412 kb on + strandat 63.502 kb on + strandat 63.539 kb on - strandat 63.539 kb on - strandat 63.539 kb on - strandat 63.737 kb on + strandat 63.738 kb on - strandat 63.738 kb on - strandat 64.015 kb on + strandat 64.015 kb on + strandat 64.016 kb on - strandat 64.016 kb on - strandat 64.016 kb on - strandat 64.221 kb on + strandat 64.221 kb on + strandat 64.221 kb on + strandat 64.222 kb on - strandat 64.465 kb on - strandat 64.605 kb on + strand, within GFF53at 64.606 kb on - strand, within GFF53at 64.719 kb on + strand, within GFF53at 64.720 kb on - strand, within GFF53at 65.124 kb on + strand, within GFF53at 65.124 kb on + strand, within GFF53at 65.124 kb on + strand, within GFF53at 65.124 kb on + strand, within GFF53at 65.125 kb on - strand, within GFF53at 65.274 kb on + strand, within GFF53at 65.275 kb on - strand, within GFF53at 65.340 kb on + strand, within GFF53at 65.341 kb on - strand, within GFF53at 65.604 kb on + strand, within GFF53at 65.604 kb on + strand, within GFF53at 65.605 kb on - strand, within GFF53at 65.605 kb on - strand, within GFF53at 65.751 kb on + strand, within GFF53at 65.752 kb on - strand, within GFF53at 65.752 kb on - strand, within GFF53at 65.752 kb on - strand, within GFF53at 65.796 kb on + strand, within GFF53at 65.979 kb on + strand, within GFF53at 65.979 kb on + strand, within GFF53at 65.979 kb on + strand, within GFF53at 65.979 kb on + strand, within GFF53at 65.980 kb on - strand, within GFF53at 65.980 kb on - strand, within GFF53at 65.980 kb on - strand, within GFF53at 65.980 kb on - strand, within GFF53at 65.980 kb on - strand, within GFF53at 65.988 kb on + strand, within GFF53at 65.989 kb on - strand, within GFF53at 65.989 kb on - strand, within GFF53at 65.989 kb on - strand, within GFF53at 65.989 kb on - strand, within GFF53at 65.989 kb on - strand, within GFF53at 66.400 kb on - strand, within GFF53at 66.664 kb on - strand, within GFF53at 66.861 kb on + strand, within GFF53at 66.862 kb on - strand, within GFF53at 66.862 kb on - strand, within GFF53at 67.417 kb on - strand, within GFF53at 67.813 kb on - strand, within GFF53at 67.875 kb on + strand, within GFF53at 68.286 kb on + strand, within GFF53at 68.287 kb on - strand, within GFF53at 68.287 kb on - strand, within GFF53at 68.319 kb on + strand, within GFF53at 68.320 kb on - strand, within GFF53at 68.547 kb on + strand, within GFF53at 68.547 kb on + strand, within GFF53at 68.548 kb on - strand, within GFF53at 68.548 kb on - strand, within GFF53at 68.562 kb on + strand, within GFF53at 68.562 kb on + strand, within GFF53at 68.562 kb on + strand, within GFF53at 68.563 kb on - strand, within GFF53at 68.563 kb on - strand, within GFF53at 68.563 kb on - strand, within GFF53at 68.599 kb on - strand, within GFF53at 68.599 kb on - strand, within GFF53at 68.599 kb on - strand, within GFF53at 68.649 kb on + strandat 68.649 kb on + strandat 68.649 kb on + strandat 68.649 kb on + strandat 68.649 kb on + strandat 68.650 kb on - strandat 68.686 kb on - strandat 68.686 kb on - strandat 68.686 kb on - strandat 68.947 kb on - strandat 70.057 kb on + strand, within GFF55at 70.058 kb on - strand, within GFF55

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL
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63,172 + GFF52 0.87 -0.4
63,412 + -1.2
63,412 + -1.0
63,502 + +0.6
63,539 - -1.3
63,539 - -2.2
63,539 - -1.0
63,737 + +0.4
63,738 - -0.7
63,738 - -0.7
64,015 + -0.6
64,015 + +0.8
64,016 - -0.6
64,016 - -0.7
64,016 - +0.1
64,221 + -0.8
64,221 + +0.1
64,221 + -0.2
64,222 - +1.3
64,465 - -0.2
64,605 + GFF53 0.10 -0.6
64,606 - GFF53 0.10 +0.0
64,719 + GFF53 0.13 +0.2
64,720 - GFF53 0.13 -0.2
65,124 + GFF53 0.21 +0.1
65,124 + GFF53 0.21 -0.3
65,124 + GFF53 0.21 -0.2
65,124 + GFF53 0.21 -0.3
65,125 - GFF53 0.21 -1.3
65,274 + GFF53 0.24 +2.0
65,275 - GFF53 0.24 -0.6
65,340 + GFF53 0.25 +0.5
65,341 - GFF53 0.25 +0.3
65,604 + GFF53 0.30 +0.1
65,604 + GFF53 0.30 -0.4
65,605 - GFF53 0.30 -1.1
65,605 - GFF53 0.30 -0.1
65,751 + GFF53 0.33 +1.0
65,752 - GFF53 0.33 -0.5
65,752 - GFF53 0.33 +0.1
65,752 - GFF53 0.33 +0.6
65,796 + GFF53 0.34 -0.9
65,979 + GFF53 0.38 -0.5
65,979 + GFF53 0.38 +0.5
65,979 + GFF53 0.38 -0.7
65,979 + GFF53 0.38 +0.4
65,980 - GFF53 0.38 -0.5
65,980 - GFF53 0.38 +1.4
65,980 - GFF53 0.38 -0.2
65,980 - GFF53 0.38 +0.4
65,980 - GFF53 0.38 +0.3
65,988 + GFF53 0.38 +0.4
65,989 - GFF53 0.38 +0.1
65,989 - GFF53 0.38 -0.7
65,989 - GFF53 0.38 -0.0
65,989 - GFF53 0.38 -1.2
65,989 - GFF53 0.38 -0.5
66,400 - GFF53 0.46 -0.1
66,664 - GFF53 0.51 +0.1
66,861 + GFF53 0.55 -0.6
66,862 - GFF53 0.55 -0.0
66,862 - GFF53 0.55 +0.7
67,417 - GFF53 0.66 +1.4
67,813 - GFF53 0.74 +0.4
67,875 + GFF53 0.75 -0.1
68,286 + GFF53 0.84 -0.6
68,287 - GFF53 0.84 -0.4
68,287 - GFF53 0.84 +0.9
68,319 + GFF53 0.84 -0.6
68,320 - GFF53 0.84 +0.7
68,547 + GFF53 0.89 +1.4
68,547 + GFF53 0.89 -0.2
68,548 - GFF53 0.89 -0.5
68,548 - GFF53 0.89 +1.4
68,562 + GFF53 0.89 +1.5
68,562 + GFF53 0.89 -0.0
68,562 + GFF53 0.89 -1.0
68,563 - GFF53 0.89 -0.2
68,563 - GFF53 0.89 -0.3
68,563 - GFF53 0.89 +0.5
68,599 - GFF53 0.90 -0.3
68,599 - GFF53 0.90 +0.8
68,599 - GFF53 0.90 -0.9
68,649 + -0.3
68,649 + +0.4
68,649 + -0.3
68,649 + +0.2
68,649 + -0.9
68,650 - -1.0
68,686 - +0.4
68,686 - -0.3
68,686 - -0.1
68,947 - -1.1
70,057 + GFF55 0.25 -1.0
70,058 - GFF55 0.25 +0.2

Or see this region's nucleotide sequence