Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5209 and GFF5210 are separated by 80 nucleotides GFF5210 and GFF5211 overlap by 4 nucleotides GFF5211 and GFF5212 are separated by 32 nucleotides
GFF5209 - DUF1854 domain-containing protein, at 71,758 to 72,240
GFF5209
GFF5210 - hypothetical protein, at 72,321 to 73,031
GFF5210
GFF5211 - Alkaline phosphatase (EC 3.1.3.1), at 73,028 to 74,566
GFF5211
GFF5212 - Alkaline phosphatase (EC 3.1.3.1), at 74,599 to 76,062
GFF5212
Position (kb)
73
74
75 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 72.341 kb on - strand at 72.356 kb on - strand at 72.356 kb on - strand at 72.463 kb on + strand, within GFF5210 at 72.520 kb on + strand, within GFF5210 at 73.344 kb on + strand, within GFF5211 at 73.344 kb on + strand, within GFF5211 at 73.344 kb on + strand, within GFF5211 at 73.386 kb on + strand, within GFF5211 at 73.386 kb on + strand, within GFF5211 at 73.387 kb on - strand, within GFF5211 at 73.387 kb on - strand, within GFF5211 at 73.512 kb on + strand, within GFF5211 at 73.722 kb on + strand, within GFF5211 at 73.809 kb on + strand, within GFF5211 at 73.810 kb on - strand, within GFF5211 at 73.894 kb on - strand, within GFF5211 at 73.951 kb on - strand, within GFF5211 at 73.992 kb on + strand, within GFF5211 at 74.020 kb on - strand, within GFF5211 at 74.160 kb on + strand, within GFF5211 at 74.161 kb on - strand, within GFF5211 at 74.161 kb on - strand, within GFF5211 at 74.161 kb on - strand, within GFF5211 at 74.241 kb on + strand, within GFF5211 at 74.762 kb on + strand, within GFF5212 at 74.762 kb on + strand, within GFF5212 at 74.762 kb on + strand, within GFF5212 at 74.763 kb on - strand, within GFF5212 at 74.847 kb on - strand, within GFF5212 at 74.891 kb on + strand, within GFF5212 at 74.891 kb on + strand, within GFF5212 at 75.242 kb on + strand, within GFF5212 at 75.242 kb on + strand, within GFF5212 at 75.329 kb on + strand, within GFF5212 at 75.330 kb on - strand, within GFF5212 at 75.330 kb on - strand, within GFF5212 at 75.335 kb on + strand, within GFF5212 at 75.410 kb on + strand, within GFF5212
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 72,341 - -0.2 72,356 - -0.1 72,356 - +1.0 72,463 + GFF5210 0.20 -0.5 72,520 + GFF5210 0.28 -1.0 73,344 + GFF5211 0.21 +1.2 73,344 + GFF5211 0.21 +0.8 73,344 + GFF5211 0.21 -1.3 73,386 + GFF5211 0.23 -1.4 73,386 + GFF5211 0.23 +1.2 73,387 - GFF5211 0.23 -0.4 73,387 - GFF5211 0.23 -1.5 73,512 + GFF5211 0.31 +0.2 73,722 + GFF5211 0.45 -0.9 73,809 + GFF5211 0.51 -0.8 73,810 - GFF5211 0.51 +0.4 73,894 - GFF5211 0.56 -0.5 73,951 - GFF5211 0.60 -1.0 73,992 + GFF5211 0.63 +0.6 74,020 - GFF5211 0.64 -1.1 74,160 + GFF5211 0.74 -1.5 74,161 - GFF5211 0.74 +0.8 74,161 - GFF5211 0.74 +0.0 74,161 - GFF5211 0.74 +0.4 74,241 + GFF5211 0.79 +1.2 74,762 + GFF5212 0.11 +0.0 74,762 + GFF5212 0.11 +0.5 74,762 + GFF5212 0.11 -1.1 74,763 - GFF5212 0.11 +0.4 74,847 - GFF5212 0.17 +0.8 74,891 + GFF5212 0.20 +1.8 74,891 + GFF5212 0.20 -1.1 75,242 + GFF5212 0.44 -1.6 75,242 + GFF5212 0.44 -0.3 75,329 + GFF5212 0.50 -0.2 75,330 - GFF5212 0.50 -1.0 75,330 - GFF5212 0.50 -1.1 75,335 + GFF5212 0.50 -1.8 75,410 + GFF5212 0.55 -0.8
Or see this region's nucleotide sequence