Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5163 and GFF5164 are separated by 9 nucleotides GFF5164 and GFF5165 are separated by 9 nucleotides GFF5165 and GFF5166 are separated by 174 nucleotides GFF5166 and GFF5167 are separated by 66 nucleotides
GFF5163 - Soluble lytic murein transglycosylase (EC 4.2.2.n1), at 15,895 to 17,925
GFF5163
GFF5164 - 5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2), at 17,935 to 18,552
GFF5164
GFF5165 - Transcriptional regulator, LysR family, at 18,562 to 19,545
GFF5165
GFF5166 - hypothetical protein, at 19,720 to 20,043
GFF5166
GFF5167 - BLUF domain protein, at 20,110 to 20,514
GFF5167
Position (kb) 18
19
20 Strain fitness (log2 ratio) -2
-1
0
1
2 at 17.603 kb on + strand, within GFF5163 at 17.603 kb on + strand, within GFF5163 at 17.603 kb on + strand, within GFF5163 at 17.603 kb on + strand, within GFF5163 at 17.603 kb on + strand, within GFF5163 at 17.604 kb on - strand, within GFF5163 at 17.604 kb on - strand, within GFF5163 at 17.604 kb on - strand, within GFF5163 at 17.604 kb on - strand, within GFF5163 at 17.615 kb on + strand, within GFF5163 at 17.615 kb on + strand, within GFF5163 at 17.616 kb on - strand, within GFF5163 at 17.616 kb on - strand, within GFF5163 at 17.616 kb on - strand, within GFF5163 at 17.681 kb on + strand, within GFF5163 at 17.681 kb on + strand, within GFF5163 at 17.681 kb on + strand, within GFF5163 at 17.682 kb on - strand, within GFF5163 at 17.934 kb on + strand at 17.934 kb on + strand at 17.934 kb on + strand at 17.935 kb on - strand at 17.935 kb on - strand at 17.935 kb on - strand at 17.935 kb on - strand at 18.112 kb on + strand, within GFF5164 at 18.112 kb on + strand, within GFF5164 at 18.113 kb on - strand, within GFF5164 at 18.113 kb on - strand, within GFF5164 at 18.113 kb on - strand, within GFF5164 at 18.113 kb on - strand, within GFF5164 at 18.259 kb on + strand, within GFF5164 at 18.290 kb on - strand, within GFF5164 at 18.290 kb on - strand, within GFF5164 at 18.358 kb on + strand, within GFF5164 at 18.358 kb on + strand, within GFF5164 at 18.359 kb on - strand, within GFF5164 at 18.359 kb on - strand, within GFF5164 at 18.359 kb on - strand, within GFF5164 at 18.359 kb on - strand, within GFF5164 at 18.359 kb on - strand, within GFF5164 at 18.400 kb on + strand, within GFF5164 at 18.400 kb on + strand, within GFF5164 at 18.401 kb on - strand, within GFF5164 at 18.401 kb on - strand, within GFF5164 at 18.401 kb on - strand, within GFF5164 at 18.401 kb on - strand, within GFF5164 at 18.401 kb on - strand, within GFF5164 at 18.701 kb on + strand, within GFF5165 at 18.701 kb on + strand, within GFF5165 at 18.702 kb on - strand, within GFF5165 at 18.702 kb on - strand, within GFF5165 at 18.702 kb on - strand, within GFF5165 at 18.833 kb on + strand, within GFF5165 at 18.833 kb on + strand, within GFF5165 at 18.834 kb on - strand, within GFF5165 at 18.839 kb on + strand, within GFF5165 at 18.839 kb on + strand, within GFF5165 at 18.839 kb on + strand, within GFF5165 at 18.839 kb on + strand, within GFF5165 at 18.839 kb on + strand, within GFF5165 at 18.839 kb on + strand, within GFF5165 at 18.839 kb on + strand, within GFF5165 at 18.840 kb on - strand, within GFF5165 at 18.840 kb on - strand, within GFF5165 at 18.840 kb on - strand, within GFF5165 at 18.840 kb on - strand, within GFF5165 at 18.840 kb on - strand, within GFF5165 at 18.840 kb on - strand, within GFF5165 at 18.840 kb on - strand, within GFF5165 at 18.840 kb on - strand, within GFF5165 at 18.840 kb on - strand, within GFF5165 at 18.840 kb on - strand, within GFF5165 at 18.840 kb on - strand, within GFF5165 at 18.840 kb on - strand, within GFF5165 at 18.848 kb on + strand, within GFF5165 at 18.848 kb on + strand, within GFF5165 at 18.848 kb on + strand, within GFF5165 at 18.849 kb on - strand, within GFF5165 at 18.849 kb on - strand, within GFF5165 at 18.849 kb on - strand, within GFF5165 at 18.849 kb on - strand, within GFF5165 at 19.157 kb on - strand, within GFF5165 at 19.264 kb on + strand, within GFF5165 at 19.265 kb on - strand, within GFF5165 at 19.265 kb on - strand, within GFF5165 at 19.265 kb on - strand, within GFF5165 at 19.265 kb on - strand, within GFF5165 at 19.265 kb on - strand, within GFF5165 at 19.265 kb on - strand, within GFF5165 at 19.565 kb on + strand at 19.565 kb on + strand at 19.566 kb on - strand at 19.605 kb on + strand at 19.605 kb on + strand at 19.605 kb on + strand at 19.605 kb on + strand at 19.605 kb on + strand at 19.606 kb on - strand at 19.610 kb on + strand at 19.611 kb on - strand at 19.611 kb on - strand at 20.023 kb on + strand at 20.023 kb on + strand at 20.023 kb on + strand at 20.023 kb on + strand at 20.023 kb on + strand at 20.023 kb on + strand at 20.023 kb on + strand at 20.023 kb on + strand at 20.023 kb on + strand at 20.023 kb on + strand at 20.023 kb on + strand at 20.023 kb on + strand at 20.023 kb on + strand at 20.023 kb on + strand at 20.024 kb on - strand at 20.024 kb on - strand at 20.024 kb on - strand at 20.024 kb on - strand at 20.024 kb on - strand at 20.024 kb on - strand at 20.024 kb on - strand at 20.024 kb on - strand at 20.024 kb on - strand at 20.024 kb on - strand at 20.024 kb on - strand at 20.024 kb on - strand at 20.240 kb on + strand, within GFF5167 at 20.240 kb on + strand, within GFF5167 at 20.241 kb on - strand, within GFF5167 at 20.241 kb on - strand, within GFF5167 at 20.267 kb on + strand, within GFF5167 at 20.267 kb on + strand, within GFF5167 at 20.267 kb on + strand, within GFF5167 at 20.267 kb on + strand, within GFF5167 at 20.267 kb on + strand, within GFF5167 at 20.268 kb on - strand, within GFF5167 at 20.268 kb on - strand, within GFF5167 at 20.268 kb on - strand, within GFF5167 at 20.268 kb on - strand, within GFF5167 at 20.268 kb on - strand, within GFF5167 at 20.268 kb on - strand, within GFF5167 at 20.480 kb on + strand at 20.480 kb on + strand at 20.481 kb on - strand at 20.492 kb on + strand at 20.492 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 17,603 + GFF5163 0.84 +0.2 17,603 + GFF5163 0.84 -0.1 17,603 + GFF5163 0.84 -0.2 17,603 + GFF5163 0.84 +0.2 17,603 + GFF5163 0.84 -0.6 17,604 - GFF5163 0.84 +1.2 17,604 - GFF5163 0.84 -1.3 17,604 - GFF5163 0.84 +0.1 17,604 - GFF5163 0.84 -0.8 17,615 + GFF5163 0.85 -0.0 17,615 + GFF5163 0.85 +0.2 17,616 - GFF5163 0.85 +1.8 17,616 - GFF5163 0.85 -0.3 17,616 - GFF5163 0.85 +0.2 17,681 + GFF5163 0.88 -0.5 17,681 + GFF5163 0.88 -1.0 17,681 + GFF5163 0.88 +0.2 17,682 - GFF5163 0.88 +0.1 17,934 + -1.0 17,934 + -1.2 17,934 + -0.6 17,935 - -0.2 17,935 - -1.3 17,935 - -0.4 17,935 - -0.5 18,112 + GFF5164 0.29 +0.2 18,112 + GFF5164 0.29 -0.8 18,113 - GFF5164 0.29 -1.1 18,113 - GFF5164 0.29 +0.4 18,113 - GFF5164 0.29 -0.6 18,113 - GFF5164 0.29 +0.0 18,259 + GFF5164 0.52 -0.4 18,290 - GFF5164 0.57 -0.0 18,290 - GFF5164 0.57 +1.0 18,358 + GFF5164 0.68 +0.2 18,358 + GFF5164 0.68 -1.4 18,359 - GFF5164 0.69 +0.7 18,359 - GFF5164 0.69 -0.0 18,359 - GFF5164 0.69 -0.4 18,359 - GFF5164 0.69 -0.5 18,359 - GFF5164 0.69 +1.1 18,400 + GFF5164 0.75 +0.8 18,400 + GFF5164 0.75 +1.0 18,401 - GFF5164 0.75 -0.5 18,401 - GFF5164 0.75 +0.1 18,401 - GFF5164 0.75 -0.3 18,401 - GFF5164 0.75 -0.5 18,401 - GFF5164 0.75 +0.6 18,701 + GFF5165 0.14 -0.6 18,701 + GFF5165 0.14 -0.2 18,702 - GFF5165 0.14 +0.7 18,702 - GFF5165 0.14 +0.0 18,702 - GFF5165 0.14 +0.1 18,833 + GFF5165 0.28 +0.4 18,833 + GFF5165 0.28 +1.5 18,834 - GFF5165 0.28 +1.1 18,839 + GFF5165 0.28 +0.8 18,839 + GFF5165 0.28 +1.1 18,839 + GFF5165 0.28 -2.0 18,839 + GFF5165 0.28 -0.9 18,839 + GFF5165 0.28 -0.3 18,839 + GFF5165 0.28 -0.7 18,839 + GFF5165 0.28 +0.3 18,840 - GFF5165 0.28 -0.9 18,840 - GFF5165 0.28 +0.7 18,840 - GFF5165 0.28 -0.3 18,840 - GFF5165 0.28 -0.7 18,840 - GFF5165 0.28 -0.8 18,840 - GFF5165 0.28 +0.2 18,840 - GFF5165 0.28 +1.8 18,840 - GFF5165 0.28 +1.4 18,840 - GFF5165 0.28 +0.1 18,840 - GFF5165 0.28 -0.2 18,840 - GFF5165 0.28 -0.7 18,840 - GFF5165 0.28 -0.6 18,848 + GFF5165 0.29 +1.0 18,848 + GFF5165 0.29 -0.2 18,848 + GFF5165 0.29 +0.1 18,849 - GFF5165 0.29 +0.9 18,849 - GFF5165 0.29 +0.2 18,849 - GFF5165 0.29 -1.0 18,849 - GFF5165 0.29 -0.9 19,157 - GFF5165 0.60 +0.1 19,264 + GFF5165 0.71 -0.8 19,265 - GFF5165 0.71 -0.1 19,265 - GFF5165 0.71 -0.0 19,265 - GFF5165 0.71 +0.7 19,265 - GFF5165 0.71 +0.7 19,265 - GFF5165 0.71 +0.9 19,265 - GFF5165 0.71 +0.8 19,565 + +0.5 19,565 + +0.5 19,566 - +0.5 19,605 + -0.4 19,605 + -0.7 19,605 + -1.5 19,605 + -0.1 19,605 + -1.6 19,606 - +0.8 19,610 + -1.6 19,611 - -1.2 19,611 - -1.3 20,023 + +0.0 20,023 + +0.5 20,023 + -0.4 20,023 + -0.4 20,023 + +0.1 20,023 + -0.2 20,023 + -0.0 20,023 + -0.6 20,023 + +0.1 20,023 + -0.3 20,023 + +0.2 20,023 + +1.6 20,023 + +0.0 20,023 + +1.8 20,024 - +0.4 20,024 - +0.6 20,024 - -0.1 20,024 - -0.2 20,024 - +0.8 20,024 - -0.3 20,024 - -0.3 20,024 - +0.8 20,024 - -0.4 20,024 - -0.6 20,024 - -1.2 20,024 - +0.1 20,240 + GFF5167 0.32 -2.1 20,240 + GFF5167 0.32 +2.2 20,241 - GFF5167 0.32 -0.6 20,241 - GFF5167 0.32 -1.5 20,267 + GFF5167 0.39 +1.8 20,267 + GFF5167 0.39 -0.9 20,267 + GFF5167 0.39 -0.2 20,267 + GFF5167 0.39 +1.0 20,267 + GFF5167 0.39 -0.2 20,268 - GFF5167 0.39 -1.3 20,268 - GFF5167 0.39 +1.5 20,268 - GFF5167 0.39 +0.6 20,268 - GFF5167 0.39 +0.5 20,268 - GFF5167 0.39 -0.5 20,268 - GFF5167 0.39 +0.3 20,480 + +0.5 20,480 + -1.3 20,481 - -0.7 20,492 + +0.1 20,492 + +0.2
Or see this region's nucleotide sequence