Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4717 and GFF4718 are separated by 1 nucleotides GFF4718 and GFF4719 are separated by 16 nucleotides GFF4719 and GFF4720 overlap by 4 nucleotides
GFF4717 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 79,393 to 80,190
GFF4717
GFF4718 - ABC transporter, ATP-binding protein (cluster 3, basic aa/glutamine/opines), at 80,192 to 80,962
GFF4718
GFF4719 - hypothetical protein, at 80,979 to 81,647
GFF4719
GFF4720 - Putrescine transport system permease protein potI, at 81,644 to 82,534
GFF4720
Position (kb) 80
81
82 Strain fitness (log2 ratio) -2
-1
0
1 at 80.044 kb on + strand, within GFF4717 at 80.045 kb on - strand, within GFF4717 at 80.045 kb on - strand, within GFF4717 at 80.074 kb on + strand, within GFF4717 at 80.074 kb on + strand, within GFF4717 at 80.075 kb on - strand, within GFF4717 at 80.075 kb on - strand, within GFF4717 at 80.075 kb on - strand, within GFF4717 at 80.075 kb on - strand, within GFF4717 at 80.075 kb on - strand, within GFF4717 at 80.093 kb on - strand, within GFF4717 at 80.093 kb on - strand, within GFF4717 at 80.093 kb on - strand, within GFF4717 at 80.113 kb on + strand at 80.114 kb on - strand at 80.114 kb on - strand at 80.114 kb on - strand at 80.114 kb on - strand at 80.114 kb on - strand at 80.179 kb on + strand at 80.621 kb on + strand, within GFF4718 at 80.621 kb on + strand, within GFF4718 at 80.622 kb on - strand, within GFF4718 at 80.622 kb on - strand, within GFF4718 at 80.622 kb on - strand, within GFF4718 at 80.622 kb on - strand, within GFF4718 at 80.960 kb on + strand at 80.961 kb on - strand at 80.961 kb on - strand at 80.961 kb on - strand at 81.238 kb on - strand, within GFF4719 at 81.238 kb on - strand, within GFF4719 at 81.238 kb on - strand, within GFF4719 at 81.238 kb on - strand, within GFF4719 at 81.386 kb on + strand, within GFF4719 at 81.433 kb on + strand, within GFF4719 at 81.434 kb on - strand, within GFF4719 at 81.434 kb on - strand, within GFF4719 at 81.562 kb on - strand, within GFF4719 at 81.744 kb on + strand, within GFF4720 at 81.744 kb on + strand, within GFF4720 at 81.745 kb on - strand, within GFF4720 at 81.748 kb on + strand, within GFF4720 at 81.748 kb on + strand, within GFF4720 at 81.749 kb on - strand, within GFF4720 at 81.749 kb on - strand, within GFF4720 at 81.749 kb on - strand, within GFF4720 at 81.749 kb on - strand, within GFF4720 at 81.749 kb on - strand, within GFF4720 at 81.960 kb on + strand, within GFF4720 at 81.961 kb on - strand, within GFF4720 at 82.071 kb on + strand, within GFF4720 at 82.194 kb on - strand, within GFF4720 at 82.452 kb on + strand at 82.520 kb on + strand at 82.520 kb on + strand at 82.521 kb on - strand at 82.521 kb on - strand at 82.613 kb on - strand at 82.613 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 80,044 + GFF4717 0.82 -0.3 80,045 - GFF4717 0.82 -0.4 80,045 - GFF4717 0.82 -0.8 80,074 + GFF4717 0.85 -0.2 80,074 + GFF4717 0.85 +1.1 80,075 - GFF4717 0.85 +1.3 80,075 - GFF4717 0.85 -0.4 80,075 - GFF4717 0.85 +0.5 80,075 - GFF4717 0.85 -0.0 80,075 - GFF4717 0.85 -0.3 80,093 - GFF4717 0.88 +0.5 80,093 - GFF4717 0.88 -0.1 80,093 - GFF4717 0.88 +0.4 80,113 + -0.1 80,114 - +1.0 80,114 - -1.5 80,114 - -1.5 80,114 - +0.1 80,114 - -0.8 80,179 + +0.5 80,621 + GFF4718 0.56 -0.1 80,621 + GFF4718 0.56 -0.6 80,622 - GFF4718 0.56 -0.2 80,622 - GFF4718 0.56 +0.2 80,622 - GFF4718 0.56 +0.3 80,622 - GFF4718 0.56 +0.5 80,960 + -1.3 80,961 - +0.0 80,961 - -0.3 80,961 - +0.3 81,238 - GFF4719 0.39 -0.9 81,238 - GFF4719 0.39 -0.7 81,238 - GFF4719 0.39 -2.1 81,238 - GFF4719 0.39 -0.8 81,386 + GFF4719 0.61 +0.0 81,433 + GFF4719 0.68 +0.4 81,434 - GFF4719 0.68 -0.3 81,434 - GFF4719 0.68 -1.0 81,562 - GFF4719 0.87 -0.8 81,744 + GFF4720 0.11 -0.6 81,744 + GFF4720 0.11 +0.1 81,745 - GFF4720 0.11 -0.6 81,748 + GFF4720 0.12 +0.5 81,748 + GFF4720 0.12 -0.6 81,749 - GFF4720 0.12 -0.0 81,749 - GFF4720 0.12 +0.3 81,749 - GFF4720 0.12 -0.5 81,749 - GFF4720 0.12 +0.4 81,749 - GFF4720 0.12 -0.8 81,960 + GFF4720 0.35 -0.3 81,961 - GFF4720 0.36 -0.0 82,071 + GFF4720 0.48 +0.1 82,194 - GFF4720 0.62 -1.0 82,452 + -1.8 82,520 + -0.4 82,520 + -0.7 82,521 - -0.9 82,521 - -0.5 82,613 - -0.1 82,613 - -0.6
Or see this region's nucleotide sequence