Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4414 and GFF4415 overlap by 4 nucleotides GFF4415 and GFF4416 overlap by 4 nucleotides GFF4416 and GFF4417 are separated by 12 nucleotides
GFF4414 - Conjugative transfer protein TrbB, at 50,950 to 52,014
GFF4414
GFF4415 - Conjugative transfer protein TrbC, at 52,011 to 52,400
GFF4415
GFF4416 - Conjugative transfer protein TrbD, at 52,397 to 52,669
GFF4416
GFF4417 - Conjugative transfer protein TrbE, at 52,682 to 55,156
GFF4417
Position (kb)
52
53 Strain fitness (log2 ratio)
-2
-1
0
1 at 51.474 kb on - strand, within GFF4414 at 51.474 kb on - strand, within GFF4414 at 51.474 kb on - strand, within GFF4414 at 51.474 kb on - strand, within GFF4414 at 51.889 kb on + strand, within GFF4414 at 51.983 kb on + strand at 51.984 kb on - strand at 51.984 kb on - strand at 52.045 kb on + strand at 52.045 kb on + strand at 52.045 kb on + strand at 52.100 kb on + strand, within GFF4415 at 52.100 kb on + strand, within GFF4415 at 52.396 kb on + strand at 52.396 kb on + strand at 52.411 kb on + strand at 52.411 kb on + strand at 52.498 kb on + strand, within GFF4416 at 52.591 kb on - strand, within GFF4416 at 52.940 kb on + strand, within GFF4417 at 52.941 kb on - strand, within GFF4417 at 53.036 kb on + strand, within GFF4417 at 53.036 kb on + strand, within GFF4417 at 53.037 kb on - strand, within GFF4417 at 53.037 kb on - strand, within GFF4417 at 53.054 kb on + strand, within GFF4417 at 53.054 kb on + strand, within GFF4417 at 53.054 kb on + strand, within GFF4417 at 53.054 kb on + strand, within GFF4417 at 53.054 kb on + strand, within GFF4417 at 53.054 kb on + strand, within GFF4417 at 53.054 kb on + strand, within GFF4417 at 53.055 kb on - strand, within GFF4417 at 53.055 kb on - strand, within GFF4417 at 53.055 kb on - strand, within GFF4417 at 53.055 kb on - strand, within GFF4417 at 53.055 kb on - strand, within GFF4417 at 53.099 kb on + strand, within GFF4417 at 53.099 kb on + strand, within GFF4417 at 53.099 kb on + strand, within GFF4417 at 53.099 kb on + strand, within GFF4417 at 53.099 kb on + strand, within GFF4417 at 53.099 kb on + strand, within GFF4417 at 53.099 kb on + strand, within GFF4417 at 53.099 kb on + strand, within GFF4417 at 53.099 kb on + strand, within GFF4417 at 53.099 kb on + strand, within GFF4417 at 53.099 kb on + strand, within GFF4417 at 53.100 kb on - strand, within GFF4417 at 53.100 kb on - strand, within GFF4417 at 53.100 kb on - strand, within GFF4417 at 53.100 kb on - strand, within GFF4417 at 53.100 kb on - strand, within GFF4417 at 53.100 kb on - strand, within GFF4417 at 53.100 kb on - strand, within GFF4417 at 53.100 kb on - strand, within GFF4417 at 53.100 kb on - strand, within GFF4417 at 53.100 kb on - strand, within GFF4417 at 53.100 kb on - strand, within GFF4417 at 53.100 kb on - strand, within GFF4417 at 53.100 kb on - strand, within GFF4417 at 53.100 kb on - strand, within GFF4417 at 53.100 kb on - strand, within GFF4417 at 53.100 kb on - strand, within GFF4417 at 53.100 kb on - strand, within GFF4417 at 53.100 kb on - strand, within GFF4417 at 53.175 kb on + strand, within GFF4417 at 53.175 kb on + strand, within GFF4417 at 53.175 kb on + strand, within GFF4417 at 53.175 kb on + strand, within GFF4417 at 53.175 kb on + strand, within GFF4417 at 53.175 kb on + strand, within GFF4417 at 53.175 kb on + strand, within GFF4417 at 53.175 kb on + strand, within GFF4417 at 53.175 kb on + strand, within GFF4417 at 53.175 kb on + strand, within GFF4417 at 53.176 kb on - strand, within GFF4417 at 53.176 kb on - strand, within GFF4417 at 53.176 kb on - strand, within GFF4417 at 53.176 kb on - strand, within GFF4417 at 53.176 kb on - strand, within GFF4417 at 53.176 kb on - strand, within GFF4417 at 53.176 kb on - strand, within GFF4417 at 53.176 kb on - strand, within GFF4417 at 53.176 kb on - strand, within GFF4417 at 53.176 kb on - strand, within GFF4417 at 53.176 kb on - strand, within GFF4417 at 53.176 kb on - strand, within GFF4417 at 53.237 kb on + strand, within GFF4417 at 53.237 kb on + strand, within GFF4417 at 53.237 kb on + strand, within GFF4417 at 53.237 kb on + strand, within GFF4417 at 53.238 kb on - strand, within GFF4417 at 53.238 kb on - strand, within GFF4417 at 53.238 kb on - strand, within GFF4417 at 53.238 kb on - strand, within GFF4417 at 53.238 kb on - strand, within GFF4417 at 53.238 kb on - strand, within GFF4417 at 53.238 kb on - strand, within GFF4417 at 53.267 kb on + strand, within GFF4417 at 53.458 kb on + strand, within GFF4417 at 53.458 kb on + strand, within GFF4417 at 53.458 kb on + strand, within GFF4417 at 53.459 kb on - strand, within GFF4417 at 53.459 kb on - strand, within GFF4417 at 53.556 kb on + strand, within GFF4417 at 53.557 kb on - strand, within GFF4417 at 53.557 kb on - strand, within GFF4417
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 51,474 - GFF4414 0.49 -0.6 51,474 - GFF4414 0.49 -0.1 51,474 - GFF4414 0.49 -0.3 51,474 - GFF4414 0.49 -0.6 51,889 + GFF4414 0.88 -1.9 51,983 + -0.1 51,984 - +0.3 51,984 - -1.4 52,045 + -0.9 52,045 + -1.2 52,045 + -0.2 52,100 + GFF4415 0.23 -0.6 52,100 + GFF4415 0.23 -0.5 52,396 + +0.5 52,396 + +0.5 52,411 + +0.8 52,411 + -0.4 52,498 + GFF4416 0.37 -1.1 52,591 - GFF4416 0.71 -0.9 52,940 + GFF4417 0.10 -0.0 52,941 - GFF4417 0.10 +0.1 53,036 + GFF4417 0.14 -0.0 53,036 + GFF4417 0.14 +0.6 53,037 - GFF4417 0.14 +0.7 53,037 - GFF4417 0.14 +0.5 53,054 + GFF4417 0.15 -0.1 53,054 + GFF4417 0.15 -0.6 53,054 + GFF4417 0.15 -0.8 53,054 + GFF4417 0.15 +0.0 53,054 + GFF4417 0.15 +0.3 53,054 + GFF4417 0.15 +0.2 53,054 + GFF4417 0.15 -0.0 53,055 - GFF4417 0.15 +0.6 53,055 - GFF4417 0.15 -0.1 53,055 - GFF4417 0.15 -0.0 53,055 - GFF4417 0.15 -0.4 53,055 - GFF4417 0.15 -0.0 53,099 + GFF4417 0.17 -0.8 53,099 + GFF4417 0.17 -1.3 53,099 + GFF4417 0.17 -0.2 53,099 + GFF4417 0.17 +0.1 53,099 + GFF4417 0.17 +0.4 53,099 + GFF4417 0.17 -0.6 53,099 + GFF4417 0.17 -0.2 53,099 + GFF4417 0.17 +0.7 53,099 + GFF4417 0.17 +1.0 53,099 + GFF4417 0.17 -0.7 53,099 + GFF4417 0.17 +0.1 53,100 - GFF4417 0.17 -2.0 53,100 - GFF4417 0.17 -1.4 53,100 - GFF4417 0.17 -0.8 53,100 - GFF4417 0.17 -0.0 53,100 - GFF4417 0.17 +0.7 53,100 - GFF4417 0.17 +0.7 53,100 - GFF4417 0.17 -1.1 53,100 - GFF4417 0.17 -0.2 53,100 - GFF4417 0.17 +0.2 53,100 - GFF4417 0.17 +0.3 53,100 - GFF4417 0.17 -0.0 53,100 - GFF4417 0.17 -1.0 53,100 - GFF4417 0.17 -0.1 53,100 - GFF4417 0.17 +0.4 53,100 - GFF4417 0.17 -0.3 53,100 - GFF4417 0.17 -0.1 53,100 - GFF4417 0.17 -0.0 53,100 - GFF4417 0.17 +0.5 53,175 + GFF4417 0.20 +0.2 53,175 + GFF4417 0.20 -0.2 53,175 + GFF4417 0.20 -0.7 53,175 + GFF4417 0.20 -0.4 53,175 + GFF4417 0.20 -0.7 53,175 + GFF4417 0.20 -0.3 53,175 + GFF4417 0.20 -0.2 53,175 + GFF4417 0.20 +0.2 53,175 + GFF4417 0.20 -1.5 53,175 + GFF4417 0.20 +1.0 53,176 - GFF4417 0.20 -0.3 53,176 - GFF4417 0.20 +0.6 53,176 - GFF4417 0.20 -0.8 53,176 - GFF4417 0.20 -0.8 53,176 - GFF4417 0.20 -0.3 53,176 - GFF4417 0.20 +0.2 53,176 - GFF4417 0.20 -0.5 53,176 - GFF4417 0.20 +0.0 53,176 - GFF4417 0.20 -1.2 53,176 - GFF4417 0.20 -0.4 53,176 - GFF4417 0.20 -0.3 53,176 - GFF4417 0.20 -0.6 53,237 + GFF4417 0.22 -1.1 53,237 + GFF4417 0.22 +0.1 53,237 + GFF4417 0.22 +0.0 53,237 + GFF4417 0.22 +0.6 53,238 - GFF4417 0.22 -1.8 53,238 - GFF4417 0.22 +0.7 53,238 - GFF4417 0.22 -0.2 53,238 - GFF4417 0.22 -0.7 53,238 - GFF4417 0.22 +0.2 53,238 - GFF4417 0.22 -0.5 53,238 - GFF4417 0.22 -0.7 53,267 + GFF4417 0.24 -0.1 53,458 + GFF4417 0.31 -0.1 53,458 + GFF4417 0.31 -1.0 53,458 + GFF4417 0.31 +0.6 53,459 - GFF4417 0.31 +0.0 53,459 - GFF4417 0.31 -0.2 53,556 + GFF4417 0.35 -0.9 53,557 - GFF4417 0.35 -0.5 53,557 - GFF4417 0.35 +0.7
Or see this region's nucleotide sequence