Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4330 and GFF4331 are separated by 285 nucleotides GFF4331 and GFF4332 are separated by 75 nucleotides
GFF4330 - Acyl-CoA thioesterase II (EC 3.1.2.-), at 252,041 to 252,949
GFF4330
GFF4331 - Oxidoreductase, GMC family, at 253,235 to 254,977
GFF4331
GFF4332 - Aldehyde dehydrogenase (EC 1.2.1.3), at 255,053 to 256,540
GFF4332
Position (kb) 253
254
255 Strain fitness (log2 ratio) -1
0
1 at 252.913 kb on + strand at 252.913 kb on + strand at 252.947 kb on + strand at 253.033 kb on + strand at 253.034 kb on - strand at 253.052 kb on - strand at 253.052 kb on - strand at 253.062 kb on + strand at 253.114 kb on + strand at 253.114 kb on + strand at 253.217 kb on - strand at 253.254 kb on + strand at 253.255 kb on - strand at 253.255 kb on - strand at 253.255 kb on - strand at 253.262 kb on + strand at 253.262 kb on + strand at 253.434 kb on - strand, within GFF4331 at 253.541 kb on + strand, within GFF4331 at 253.541 kb on + strand, within GFF4331 at 253.541 kb on + strand, within GFF4331 at 253.566 kb on - strand, within GFF4331 at 253.604 kb on + strand, within GFF4331 at 253.604 kb on + strand, within GFF4331 at 253.811 kb on + strand, within GFF4331 at 254.694 kb on - strand, within GFF4331 at 254.928 kb on + strand at 255.035 kb on - strand at 255.039 kb on - strand at 255.336 kb on - strand, within GFF4332 at 255.336 kb on - strand, within GFF4332 at 255.431 kb on + strand, within GFF4332 at 255.501 kb on - strand, within GFF4332 at 255.501 kb on - strand, within GFF4332 at 255.799 kb on - strand, within GFF4332
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 252,913 + +0.2 252,913 + -1.7 252,947 + -0.2 253,033 + -1.0 253,034 - -1.5 253,052 - +1.0 253,052 - -0.3 253,062 + -0.6 253,114 + -0.7 253,114 + -0.7 253,217 - -0.8 253,254 + -0.4 253,255 - -0.1 253,255 - -1.2 253,255 - -0.4 253,262 + -0.2 253,262 + +1.0 253,434 - GFF4331 0.11 +0.3 253,541 + GFF4331 0.18 -0.4 253,541 + GFF4331 0.18 +1.3 253,541 + GFF4331 0.18 -0.2 253,566 - GFF4331 0.19 -0.7 253,604 + GFF4331 0.21 +0.3 253,604 + GFF4331 0.21 +0.6 253,811 + GFF4331 0.33 +0.0 254,694 - GFF4331 0.84 +0.5 254,928 + +0.2 255,035 - -0.1 255,039 - +0.0 255,336 - GFF4332 0.19 -0.7 255,336 - GFF4332 0.19 +0.5 255,431 + GFF4332 0.25 +0.2 255,501 - GFF4332 0.30 -0.6 255,501 - GFF4332 0.30 +0.7 255,799 - GFF4332 0.50 -1.5
Or see this region's nucleotide sequence