Strain Fitness in Variovorax sp. SCN45 around GFF4312

Experiment: M9 with calcium pantothenate 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4310 and GFF4311 overlap by 8 nucleotidesGFF4311 and GFF4312 are separated by 63 nucleotidesGFF4312 and GFF4313 are separated by 10 nucleotides GFF4310 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 230,198 to 232,054 GFF4310 GFF4311 - Enoyl-CoA hydratase (EC 4.2.1.17), at 232,047 to 232,892 GFF4311 GFF4312 - Citronellol dehydrogenase; Citronellal dehydrogenase, at 232,956 to 233,825 GFF4312 GFF4313 - 2-dehydropantoate 2-reductase (EC 1.1.1.169), at 233,836 to 234,840 GFF4313 Position (kb) 232 233 234Strain fitness (log2 ratio) -2 -1 0 1 2at 232.046 kb on + strandat 232.952 kb on + strandat 232.975 kb on - strandat 233.263 kb on - strand, within GFF4312at 233.263 kb on - strand, within GFF4312at 233.355 kb on + strand, within GFF4312at 233.355 kb on + strand, within GFF4312at 233.356 kb on - strand, within GFF4312at 233.356 kb on - strand, within GFF4312at 233.364 kb on + strand, within GFF4312at 233.563 kb on - strand, within GFF4312at 233.683 kb on - strand, within GFF4312at 234.515 kb on + strand, within GFF4313at 234.516 kb on - strand, within GFF4313at 234.630 kb on - strand, within GFF4313at 234.630 kb on - strand, within GFF4313at 234.630 kb on - strand, within GFF4313at 234.630 kb on - strand, within GFF4313at 234.630 kb on - strand, within GFF4313at 234.824 kb on + strandat 234.824 kb on + strandat 234.825 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL
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232,046 + -1.5
232,952 + +1.8
232,975 - -0.5
233,263 - GFF4312 0.35 +0.2
233,263 - GFF4312 0.35 -0.2
233,355 + GFF4312 0.46 -0.2
233,355 + GFF4312 0.46 -0.4
233,356 - GFF4312 0.46 -2.1
233,356 - GFF4312 0.46 +0.0
233,364 + GFF4312 0.47 -1.0
233,563 - GFF4312 0.70 -0.8
233,683 - GFF4312 0.84 -0.4
234,515 + GFF4313 0.68 +1.0
234,516 - GFF4313 0.68 -0.4
234,630 - GFF4313 0.79 +0.2
234,630 - GFF4313 0.79 -1.0
234,630 - GFF4313 0.79 -0.4
234,630 - GFF4313 0.79 -1.2
234,630 - GFF4313 0.79 +0.4
234,824 + -1.4
234,824 + -0.8
234,825 - +1.8

Or see this region's nucleotide sequence