Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4310 and GFF4311 overlap by 8 nucleotides GFF4311 and GFF4312 are separated by 63 nucleotides GFF4312 and GFF4313 are separated by 10 nucleotides
GFF4310 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 230,198 to 232,054
GFF4310
GFF4311 - Enoyl-CoA hydratase (EC 4.2.1.17), at 232,047 to 232,892
GFF4311
GFF4312 - Citronellol dehydrogenase; Citronellal dehydrogenase, at 232,956 to 233,825
GFF4312
GFF4313 - 2-dehydropantoate 2-reductase (EC 1.1.1.169), at 233,836 to 234,840
GFF4313
Position (kb)
232
233
234 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 232.046 kb on + strand at 232.952 kb on + strand at 232.975 kb on - strand at 233.263 kb on - strand, within GFF4312 at 233.263 kb on - strand, within GFF4312 at 233.355 kb on + strand, within GFF4312 at 233.355 kb on + strand, within GFF4312 at 233.356 kb on - strand, within GFF4312 at 233.356 kb on - strand, within GFF4312 at 233.364 kb on + strand, within GFF4312 at 233.563 kb on - strand, within GFF4312 at 233.683 kb on - strand, within GFF4312 at 234.515 kb on + strand, within GFF4313 at 234.516 kb on - strand, within GFF4313 at 234.630 kb on - strand, within GFF4313 at 234.630 kb on - strand, within GFF4313 at 234.630 kb on - strand, within GFF4313 at 234.630 kb on - strand, within GFF4313 at 234.630 kb on - strand, within GFF4313 at 234.824 kb on + strand at 234.824 kb on + strand at 234.825 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 232,046 + -1.5 232,952 + +1.8 232,975 - -0.5 233,263 - GFF4312 0.35 +0.2 233,263 - GFF4312 0.35 -0.2 233,355 + GFF4312 0.46 -0.2 233,355 + GFF4312 0.46 -0.4 233,356 - GFF4312 0.46 -2.1 233,356 - GFF4312 0.46 +0.0 233,364 + GFF4312 0.47 -1.0 233,563 - GFF4312 0.70 -0.8 233,683 - GFF4312 0.84 -0.4 234,515 + GFF4313 0.68 +1.0 234,516 - GFF4313 0.68 -0.4 234,630 - GFF4313 0.79 +0.2 234,630 - GFF4313 0.79 -1.0 234,630 - GFF4313 0.79 -0.4 234,630 - GFF4313 0.79 -1.2 234,630 - GFF4313 0.79 +0.4 234,824 + -1.4 234,824 + -0.8 234,825 - +1.8
Or see this region's nucleotide sequence