Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4297 and GFF4298 are separated by 42 nucleotides GFF4298 and GFF4299 are separated by 58 nucleotides GFF4299 and GFF4300 overlap by 1 nucleotides
GFF4297 - 2-ketocyclohexanecarboxyl-CoA hydrolase, at 216,333 to 217,127
GFF4297
GFF4298 - 2-hydroxycyclohexanecarboxyl-CoA dehydrogenase, at 217,170 to 217,946
GFF4298
GFF4299 - 3-ketoacyl-CoA thiolase, at 218,005 to 219,210
GFF4299
GFF4300 - Hydroxymethylglutaryl-CoA synthase (EC 2.3.3.10) / conserved protein associated with acetyl-CoA C-acyltransferase, at 219,210 to 220,691
GFF4300
Position (kb) 218
219
220 Strain fitness (log2 ratio) -1
0
1 at 217.049 kb on - strand at 217.115 kb on - strand at 217.474 kb on + strand, within GFF4298 at 218.135 kb on + strand, within GFF4299 at 218.136 kb on - strand, within GFF4299 at 218.144 kb on + strand, within GFF4299 at 218.580 kb on - strand, within GFF4299 at 218.747 kb on + strand, within GFF4299 at 219.254 kb on + strand at 219.281 kb on - strand at 219.499 kb on + strand, within GFF4300 at 219.500 kb on - strand, within GFF4300 at 219.500 kb on - strand, within GFF4300 at 219.500 kb on - strand, within GFF4300 at 219.718 kb on + strand, within GFF4300 at 219.719 kb on - strand, within GFF4300 at 219.719 kb on - strand, within GFF4300 at 219.719 kb on - strand, within GFF4300 at 220.069 kb on + strand, within GFF4300
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 217,049 - +0.6 217,115 - +0.6 217,474 + GFF4298 0.39 -0.8 218,135 + GFF4299 0.11 -0.0 218,136 - GFF4299 0.11 -0.4 218,144 + GFF4299 0.12 -0.5 218,580 - GFF4299 0.48 -0.0 218,747 + GFF4299 0.62 -0.9 219,254 + +0.3 219,281 - -0.5 219,499 + GFF4300 0.20 -1.4 219,500 - GFF4300 0.20 +0.6 219,500 - GFF4300 0.20 -0.9 219,500 - GFF4300 0.20 -0.5 219,718 + GFF4300 0.34 -1.1 219,719 - GFF4300 0.34 +1.6 219,719 - GFF4300 0.34 +0.3 219,719 - GFF4300 0.34 -0.3 220,069 + GFF4300 0.58 -0.5
Or see this region's nucleotide sequence