Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4271 and GFF4272 are separated by 96 nucleotides GFF4272 and GFF4273 are separated by 8 nucleotides
GFF4271 - Transcriptional regulator, LysR family, at 191,279 to 192,205
GFF4271
GFF4272 - BUG/TctC family periplasmic protein, at 192,302 to 193,282
GFF4272
GFF4273 - BUG/TctC family periplasmic protein, at 193,291 to 194,259
GFF4273
Position (kb)
192
193
194 Strain fitness (log2 ratio)
-1
0
1
2 at 191.789 kb on + strand, within GFF4271 at 192.123 kb on + strand at 192.123 kb on + strand at 192.123 kb on + strand at 192.123 kb on + strand at 192.123 kb on + strand at 192.124 kb on - strand at 192.124 kb on - strand at 192.124 kb on - strand at 192.124 kb on - strand at 192.182 kb on - strand at 192.182 kb on - strand at 192.232 kb on - strand at 192.232 kb on - strand at 192.410 kb on + strand, within GFF4272 at 192.533 kb on + strand, within GFF4272 at 192.533 kb on + strand, within GFF4272 at 192.533 kb on + strand, within GFF4272 at 192.533 kb on + strand, within GFF4272 at 192.534 kb on - strand, within GFF4272 at 192.534 kb on - strand, within GFF4272 at 192.534 kb on - strand, within GFF4272 at 192.534 kb on - strand, within GFF4272 at 192.612 kb on - strand, within GFF4272 at 192.612 kb on - strand, within GFF4272 at 192.765 kb on - strand, within GFF4272 at 192.765 kb on - strand, within GFF4272 at 192.765 kb on - strand, within GFF4272 at 192.765 kb on - strand, within GFF4272 at 192.765 kb on - strand, within GFF4272 at 192.765 kb on - strand, within GFF4272 at 193.386 kb on - strand at 193.386 kb on - strand at 193.782 kb on - strand, within GFF4273 at 193.782 kb on - strand, within GFF4273 at 193.782 kb on - strand, within GFF4273 at 193.805 kb on - strand, within GFF4273 at 193.997 kb on + strand, within GFF4273 at 193.998 kb on - strand, within GFF4273 at 194.276 kb on + strand at 194.276 kb on + strand at 194.276 kb on + strand at 194.277 kb on - strand at 194.277 kb on - strand at 194.277 kb on - strand at 194.277 kb on - strand at 194.278 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 191,789 + GFF4271 0.55 -0.2 192,123 + -0.2 192,123 + -0.6 192,123 + -1.6 192,123 + +0.2 192,123 + -1.3 192,124 - -0.5 192,124 - -0.0 192,124 - -0.0 192,124 - +0.2 192,182 - -0.5 192,182 - +0.7 192,232 - +1.8 192,232 - -0.5 192,410 + GFF4272 0.11 +0.2 192,533 + GFF4272 0.24 -1.0 192,533 + GFF4272 0.24 +0.0 192,533 + GFF4272 0.24 -0.4 192,533 + GFF4272 0.24 -0.8 192,534 - GFF4272 0.24 -0.2 192,534 - GFF4272 0.24 +1.4 192,534 - GFF4272 0.24 +0.4 192,534 - GFF4272 0.24 +0.0 192,612 - GFF4272 0.32 +0.0 192,612 - GFF4272 0.32 -0.1 192,765 - GFF4272 0.47 +0.8 192,765 - GFF4272 0.47 -0.2 192,765 - GFF4272 0.47 +0.0 192,765 - GFF4272 0.47 +1.8 192,765 - GFF4272 0.47 -0.3 192,765 - GFF4272 0.47 -1.5 193,386 - -0.0 193,386 - -1.0 193,782 - GFF4273 0.51 +0.0 193,782 - GFF4273 0.51 -0.3 193,782 - GFF4273 0.51 -0.9 193,805 - GFF4273 0.53 +0.2 193,997 + GFF4273 0.73 +0.4 193,998 - GFF4273 0.73 -1.0 194,276 + -0.5 194,276 + -1.0 194,276 + -0.4 194,277 - +0.5 194,277 - -1.3 194,277 - +0.4 194,277 - -0.2 194,278 + +0.3
Or see this region's nucleotide sequence