Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF421 and GFF423 are separated by 31 nucleotides GFF423 and GFF424 overlap by 4 nucleotides GFF424 and GFF425 overlap by 4 nucleotides
GFF421 - hypothetical protein, at 456,444 to 459,227
GFF421
GFF423 - Alpha-ribazole-5'-phosphate phosphatase (EC 3.1.3.73), at 459,259 to 459,822
GFF423
GFF424 - Cobalamin synthase (EC 2.7.8.26), at 459,819 to 460,613
GFF424
GFF425 - Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (EC 2.4.2.21), at 460,610 to 461,659
GFF425
Position (kb)
459
460
461 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.144 kb on + strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.145 kb on - strand at 459.225 kb on + strand at 459.225 kb on + strand at 459.225 kb on + strand at 459.225 kb on + strand at 459.225 kb on + strand at 459.225 kb on + strand at 459.225 kb on + strand at 459.225 kb on + strand at 459.226 kb on - strand at 459.226 kb on - strand at 459.226 kb on - strand at 459.226 kb on - strand at 459.226 kb on - strand at 459.226 kb on - strand at 459.226 kb on - strand at 459.576 kb on - strand, within GFF423 at 459.764 kb on + strand, within GFF423 at 459.765 kb on - strand, within GFF423 at 459.765 kb on - strand, within GFF423 at 459.844 kb on + strand at 459.844 kb on + strand at 459.844 kb on + strand at 459.845 kb on - strand at 459.845 kb on - strand at 459.845 kb on - strand at 460.006 kb on - strand, within GFF424 at 460.006 kb on - strand, within GFF424 at 460.006 kb on - strand, within GFF424 at 460.102 kb on + strand, within GFF424 at 460.102 kb on + strand, within GFF424 at 460.102 kb on + strand, within GFF424 at 460.103 kb on - strand, within GFF424 at 460.103 kb on - strand, within GFF424 at 460.103 kb on - strand, within GFF424 at 460.103 kb on - strand, within GFF424 at 460.103 kb on - strand, within GFF424 at 460.103 kb on - strand, within GFF424 at 460.103 kb on - strand, within GFF424
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 459,144 + -0.5 459,144 + -0.6 459,144 + +0.6 459,144 + +0.2 459,144 + +0.4 459,144 + -0.5 459,144 + -0.7 459,144 + +0.0 459,144 + -0.5 459,144 + +0.3 459,144 + -0.4 459,144 + -0.3 459,144 + -1.2 459,144 + -0.3 459,144 + +0.2 459,144 + -0.2 459,144 + -0.0 459,144 + -0.9 459,144 + -0.2 459,144 + +0.7 459,144 + -0.2 459,145 - -0.2 459,145 - -0.3 459,145 - -0.2 459,145 - -0.3 459,145 - -1.2 459,145 - +0.8 459,145 - -0.0 459,145 - -0.7 459,145 - -0.1 459,145 - +0.5 459,145 - -0.3 459,145 - +1.1 459,145 - -1.5 459,145 - -1.4 459,145 - +1.1 459,145 - +0.0 459,145 - +0.6 459,145 - +0.2 459,225 + +0.4 459,225 + -0.2 459,225 + +0.9 459,225 + -1.5 459,225 + -0.6 459,225 + -0.4 459,225 + -0.9 459,225 + +0.0 459,226 - +2.2 459,226 - +0.2 459,226 - -1.1 459,226 - +0.4 459,226 - -2.2 459,226 - -0.1 459,226 - +0.5 459,576 - GFF423 0.56 -1.2 459,764 + GFF423 0.90 -0.5 459,765 - GFF423 0.90 -1.4 459,765 - GFF423 0.90 -0.1 459,844 + -0.3 459,844 + -1.1 459,844 + -0.2 459,845 - -0.1 459,845 - +0.5 459,845 - +0.9 460,006 - GFF424 0.24 +0.0 460,006 - GFF424 0.24 -2.0 460,006 - GFF424 0.24 +0.6 460,102 + GFF424 0.36 -0.2 460,102 + GFF424 0.36 +0.2 460,102 + GFF424 0.36 -0.4 460,103 - GFF424 0.36 +0.3 460,103 - GFF424 0.36 +0.9 460,103 - GFF424 0.36 -2.1 460,103 - GFF424 0.36 +0.1 460,103 - GFF424 0.36 +0.2 460,103 - GFF424 0.36 +0.8 460,103 - GFF424 0.36 +0.1
Or see this region's nucleotide sequence