Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4135 and GFF4136 are separated by 16 nucleotides GFF4136 and GFF4137 are separated by 206 nucleotides
GFF4135 - Acyl-CoA dehydrogenase; probable dibenzothiophene desulfurization enzyme, at 41,866 to 43,104
GFF4135
GFF4136 - hypothetical protein, at 43,121 to 46,051
GFF4136
GFF4137 - Methionine ABC transporter substrate-binding protein, at 46,258 to 47,070
GFF4137
Position (kb) 43
44
45
46
47 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 42.121 kb on + strand, within GFF4135 at 42.122 kb on - strand, within GFF4135 at 42.379 kb on + strand, within GFF4135 at 42.379 kb on + strand, within GFF4135 at 42.379 kb on + strand, within GFF4135 at 42.380 kb on - strand, within GFF4135 at 42.406 kb on + strand, within GFF4135 at 42.406 kb on + strand, within GFF4135 at 42.567 kb on - strand, within GFF4135 at 42.593 kb on - strand, within GFF4135 at 43.224 kb on + strand at 43.225 kb on - strand at 43.368 kb on + strand at 43.462 kb on - strand, within GFF4136 at 43.515 kb on + strand, within GFF4136 at 43.516 kb on - strand, within GFF4136 at 43.630 kb on - strand, within GFF4136 at 43.900 kb on - strand, within GFF4136 at 44.397 kb on + strand, within GFF4136 at 44.397 kb on + strand, within GFF4136 at 44.397 kb on + strand, within GFF4136 at 44.398 kb on - strand, within GFF4136 at 44.398 kb on - strand, within GFF4136 at 44.680 kb on - strand, within GFF4136 at 44.680 kb on - strand, within GFF4136 at 44.680 kb on - strand, within GFF4136 at 44.680 kb on - strand, within GFF4136 at 44.805 kb on + strand, within GFF4136 at 44.820 kb on + strand, within GFF4136 at 44.820 kb on + strand, within GFF4136 at 44.820 kb on + strand, within GFF4136 at 44.820 kb on + strand, within GFF4136 at 44.821 kb on - strand, within GFF4136 at 44.865 kb on + strand, within GFF4136 at 46.003 kb on - strand at 46.168 kb on + strand at 46.168 kb on + strand at 46.168 kb on + strand at 46.168 kb on + strand at 46.168 kb on + strand at 46.168 kb on + strand at 46.169 kb on - strand at 46.169 kb on - strand at 46.230 kb on + strand at 46.231 kb on - strand at 46.457 kb on - strand, within GFF4137 at 46.457 kb on - strand, within GFF4137 at 46.457 kb on - strand, within GFF4137 at 47.041 kb on + strand at 47.041 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 42,121 + GFF4135 0.21 -1.5 42,122 - GFF4135 0.21 +0.6 42,379 + GFF4135 0.41 -0.1 42,379 + GFF4135 0.41 +0.6 42,379 + GFF4135 0.41 +0.4 42,380 - GFF4135 0.41 -0.7 42,406 + GFF4135 0.44 +2.0 42,406 + GFF4135 0.44 +0.2 42,567 - GFF4135 0.57 -0.8 42,593 - GFF4135 0.59 -0.2 43,224 + -0.2 43,225 - -2.1 43,368 + -0.6 43,462 - GFF4136 0.12 +0.3 43,515 + GFF4136 0.13 +1.2 43,516 - GFF4136 0.13 +0.4 43,630 - GFF4136 0.17 -0.7 43,900 - GFF4136 0.27 -0.6 44,397 + GFF4136 0.44 +0.0 44,397 + GFF4136 0.44 -0.3 44,397 + GFF4136 0.44 +0.5 44,398 - GFF4136 0.44 +3.4 44,398 - GFF4136 0.44 -0.0 44,680 - GFF4136 0.53 -0.9 44,680 - GFF4136 0.53 +0.2 44,680 - GFF4136 0.53 -0.4 44,680 - GFF4136 0.53 +0.6 44,805 + GFF4136 0.57 +1.6 44,820 + GFF4136 0.58 +0.6 44,820 + GFF4136 0.58 +0.0 44,820 + GFF4136 0.58 +0.6 44,820 + GFF4136 0.58 -0.4 44,821 - GFF4136 0.58 -1.2 44,865 + GFF4136 0.60 +0.2 46,003 - -0.2 46,168 + -0.0 46,168 + -0.5 46,168 + +2.0 46,168 + -0.0 46,168 + -2.9 46,168 + +0.6 46,169 - -0.5 46,169 - -0.4 46,230 + +0.5 46,231 - +0.4 46,457 - GFF4137 0.24 +0.1 46,457 - GFF4137 0.24 -1.1 46,457 - GFF4137 0.24 -0.2 47,041 + -0.7 47,041 + -1.0
Or see this region's nucleotide sequence