Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4128 and GFF4129 overlap by 4 nucleotides GFF4129 and GFF4130 overlap by 4 nucleotides
GFF4128 - ABC transporter, substrate-binding protein (cluster 5, nickel/peptides/opines), at 31,667 to 33,295
GFF4128
GFF4129 - ABC transporter, substrate-binding protein (cluster 5, nickel/peptides/opines), at 33,292 to 34,941
GFF4129
GFF4130 - Amidase family protein SACE_5032, at 34,938 to 36,458
GFF4130
Position (kb) 33
34
35 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 32.317 kb on - strand, within GFF4128 at 32.428 kb on + strand, within GFF4128 at 32.436 kb on + strand, within GFF4128 at 32.437 kb on - strand, within GFF4128 at 32.601 kb on + strand, within GFF4128 at 32.601 kb on + strand, within GFF4128 at 32.640 kb on + strand, within GFF4128 at 32.641 kb on - strand, within GFF4128 at 32.740 kb on - strand, within GFF4128 at 32.829 kb on + strand, within GFF4128 at 32.829 kb on + strand, within GFF4128 at 32.830 kb on - strand, within GFF4128 at 32.889 kb on + strand, within GFF4128 at 33.052 kb on - strand, within GFF4128 at 33.052 kb on - strand, within GFF4128 at 33.354 kb on + strand at 33.354 kb on + strand at 33.657 kb on - strand, within GFF4129 at 33.672 kb on - strand, within GFF4129 at 33.737 kb on + strand, within GFF4129 at 33.738 kb on - strand, within GFF4129 at 34.226 kb on + strand, within GFF4129 at 34.226 kb on + strand, within GFF4129 at 34.226 kb on + strand, within GFF4129 at 34.226 kb on + strand, within GFF4129 at 34.226 kb on + strand, within GFF4129 at 34.227 kb on - strand, within GFF4129 at 34.227 kb on - strand, within GFF4129 at 34.247 kb on + strand, within GFF4129 at 34.364 kb on + strand, within GFF4129 at 34.364 kb on + strand, within GFF4129 at 34.607 kb on + strand, within GFF4129 at 34.608 kb on - strand, within GFF4129 at 34.800 kb on - strand at 34.987 kb on + strand at 34.988 kb on - strand at 35.039 kb on - strand at 35.198 kb on - strand, within GFF4130 at 35.480 kb on - strand, within GFF4130 at 35.864 kb on - strand, within GFF4130
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 32,317 - GFF4128 0.40 +0.3 32,428 + GFF4128 0.47 +1.3 32,436 + GFF4128 0.47 +1.2 32,437 - GFF4128 0.47 +1.0 32,601 + GFF4128 0.57 -0.3 32,601 + GFF4128 0.57 -2.8 32,640 + GFF4128 0.60 -0.8 32,641 - GFF4128 0.60 +1.5 32,740 - GFF4128 0.66 +0.5 32,829 + GFF4128 0.71 +0.4 32,829 + GFF4128 0.71 -1.4 32,830 - GFF4128 0.71 +0.4 32,889 + GFF4128 0.75 -0.8 33,052 - GFF4128 0.85 -0.3 33,052 - GFF4128 0.85 -0.2 33,354 + +1.6 33,354 + +0.2 33,657 - GFF4129 0.22 -0.2 33,672 - GFF4129 0.23 -0.8 33,737 + GFF4129 0.27 -0.5 33,738 - GFF4129 0.27 -1.5 34,226 + GFF4129 0.57 +1.0 34,226 + GFF4129 0.57 -0.0 34,226 + GFF4129 0.57 -1.1 34,226 + GFF4129 0.57 -0.3 34,226 + GFF4129 0.57 +1.0 34,227 - GFF4129 0.57 +1.4 34,227 - GFF4129 0.57 -0.1 34,247 + GFF4129 0.58 +0.8 34,364 + GFF4129 0.65 -0.4 34,364 + GFF4129 0.65 +0.5 34,607 + GFF4129 0.80 +1.8 34,608 - GFF4129 0.80 -0.2 34,800 - +0.2 34,987 + -0.5 34,988 - -0.6 35,039 - -1.1 35,198 - GFF4130 0.17 +0.1 35,480 - GFF4130 0.36 -0.6 35,864 - GFF4130 0.61 -1.2
Or see this region's nucleotide sequence