Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3421 and GFF3422 are separated by 31 nucleotides GFF3422 and GFF3423 are separated by 8 nucleotides
GFF3421 - Acetoacetyl-CoA synthetase (EC 6.2.1.16), at 244,385 to 246,388
GFF3421
GFF3422 - Lipoprotein-related protein, at 246,420 to 246,842
GFF3422
GFF3423 - Adenylate cyclase (EC 4.6.1.1), at 246,851 to 248,785
GFF3423
Position (kb)
246
247 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 245.442 kb on + strand, within GFF3421 at 245.442 kb on + strand, within GFF3421 at 245.442 kb on + strand, within GFF3421 at 245.442 kb on + strand, within GFF3421 at 245.442 kb on + strand, within GFF3421 at 245.442 kb on + strand, within GFF3421 at 245.443 kb on - strand, within GFF3421 at 245.443 kb on - strand, within GFF3421 at 245.499 kb on - strand, within GFF3421 at 245.850 kb on - strand, within GFF3421 at 245.850 kb on - strand, within GFF3421 at 245.850 kb on - strand, within GFF3421 at 245.891 kb on + strand, within GFF3421 at 245.892 kb on - strand, within GFF3421 at 245.966 kb on + strand, within GFF3421 at 245.966 kb on + strand, within GFF3421 at 245.967 kb on - strand, within GFF3421 at 245.967 kb on - strand, within GFF3421 at 245.967 kb on - strand, within GFF3421 at 246.026 kb on + strand, within GFF3421 at 246.026 kb on + strand, within GFF3421 at 246.027 kb on - strand, within GFF3421 at 246.027 kb on - strand, within GFF3421 at 246.027 kb on - strand, within GFF3421 at 246.027 kb on - strand, within GFF3421 at 246.027 kb on - strand, within GFF3421 at 246.027 kb on - strand, within GFF3421 at 246.027 kb on - strand, within GFF3421 at 246.027 kb on - strand, within GFF3421 at 246.027 kb on - strand, within GFF3421 at 246.027 kb on - strand, within GFF3421 at 246.083 kb on + strand, within GFF3421 at 246.101 kb on + strand, within GFF3421 at 246.101 kb on + strand, within GFF3421 at 246.101 kb on + strand, within GFF3421 at 246.101 kb on + strand, within GFF3421 at 246.101 kb on + strand, within GFF3421 at 246.101 kb on + strand, within GFF3421 at 246.102 kb on - strand, within GFF3421 at 246.102 kb on - strand, within GFF3421 at 246.102 kb on - strand, within GFF3421 at 246.102 kb on - strand, within GFF3421 at 246.102 kb on - strand, within GFF3421 at 246.102 kb on - strand, within GFF3421 at 246.102 kb on - strand, within GFF3421 at 246.353 kb on + strand at 246.353 kb on + strand at 246.353 kb on + strand at 246.353 kb on + strand at 246.354 kb on - strand at 246.354 kb on - strand at 246.354 kb on - strand at 246.354 kb on - strand at 246.354 kb on - strand at 246.354 kb on - strand at 246.354 kb on - strand at 246.354 kb on - strand at 246.354 kb on - strand at 246.386 kb on + strand at 246.407 kb on + strand at 246.408 kb on - strand at 246.664 kb on - strand, within GFF3422 at 246.709 kb on - strand, within GFF3422 at 246.709 kb on - strand, within GFF3422 at 246.709 kb on - strand, within GFF3422 at 246.768 kb on + strand, within GFF3422 at 246.768 kb on + strand, within GFF3422 at 246.870 kb on + strand at 246.870 kb on + strand at 247.047 kb on + strand, within GFF3423 at 247.048 kb on - strand, within GFF3423 at 247.048 kb on - strand, within GFF3423 at 247.203 kb on + strand, within GFF3423 at 247.203 kb on + strand, within GFF3423 at 247.203 kb on + strand, within GFF3423 at 247.204 kb on - strand, within GFF3423 at 247.204 kb on - strand, within GFF3423 at 247.317 kb on + strand, within GFF3423 at 247.317 kb on + strand, within GFF3423 at 247.317 kb on + strand, within GFF3423 at 247.318 kb on - strand, within GFF3423 at 247.803 kb on + strand, within GFF3423 at 247.803 kb on + strand, within GFF3423 at 247.803 kb on + strand, within GFF3423 at 247.803 kb on + strand, within GFF3423 at 247.803 kb on + strand, within GFF3423 at 247.803 kb on + strand, within GFF3423 at 247.803 kb on + strand, within GFF3423 at 247.804 kb on - strand, within GFF3423 at 247.804 kb on - strand, within GFF3423 at 247.818 kb on + strand, within GFF3423 at 247.819 kb on - strand, within GFF3423 at 247.819 kb on - strand, within GFF3423
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 245,442 + GFF3421 0.53 -0.2 245,442 + GFF3421 0.53 -0.0 245,442 + GFF3421 0.53 +0.6 245,442 + GFF3421 0.53 +0.1 245,442 + GFF3421 0.53 -0.6 245,442 + GFF3421 0.53 -0.5 245,443 - GFF3421 0.53 -0.6 245,443 - GFF3421 0.53 -0.9 245,499 - GFF3421 0.56 +0.1 245,850 - GFF3421 0.73 -0.7 245,850 - GFF3421 0.73 -0.5 245,850 - GFF3421 0.73 +0.5 245,891 + GFF3421 0.75 -1.7 245,892 - GFF3421 0.75 -0.6 245,966 + GFF3421 0.79 -0.1 245,966 + GFF3421 0.79 +0.0 245,967 - GFF3421 0.79 -1.4 245,967 - GFF3421 0.79 -0.5 245,967 - GFF3421 0.79 -0.6 246,026 + GFF3421 0.82 +0.7 246,026 + GFF3421 0.82 -0.3 246,027 - GFF3421 0.82 -0.6 246,027 - GFF3421 0.82 -1.4 246,027 - GFF3421 0.82 +1.1 246,027 - GFF3421 0.82 -0.3 246,027 - GFF3421 0.82 -0.7 246,027 - GFF3421 0.82 +0.1 246,027 - GFF3421 0.82 +0.3 246,027 - GFF3421 0.82 -1.3 246,027 - GFF3421 0.82 -0.5 246,027 - GFF3421 0.82 +1.0 246,083 + GFF3421 0.85 +0.4 246,101 + GFF3421 0.86 -0.2 246,101 + GFF3421 0.86 +0.3 246,101 + GFF3421 0.86 +0.5 246,101 + GFF3421 0.86 -1.9 246,101 + GFF3421 0.86 -2.7 246,101 + GFF3421 0.86 -0.2 246,102 - GFF3421 0.86 +0.7 246,102 - GFF3421 0.86 +1.0 246,102 - GFF3421 0.86 +0.1 246,102 - GFF3421 0.86 -0.5 246,102 - GFF3421 0.86 -1.4 246,102 - GFF3421 0.86 -0.8 246,102 - GFF3421 0.86 -0.3 246,353 + +0.7 246,353 + -0.2 246,353 + -0.0 246,353 + +0.4 246,354 - +0.4 246,354 - +0.8 246,354 - -0.1 246,354 - -0.2 246,354 - -1.6 246,354 - +1.0 246,354 - +0.0 246,354 - -1.2 246,354 - -0.3 246,386 + +0.2 246,407 + -0.5 246,408 - +0.1 246,664 - GFF3422 0.58 +0.7 246,709 - GFF3422 0.68 -1.7 246,709 - GFF3422 0.68 -1.5 246,709 - GFF3422 0.68 +0.2 246,768 + GFF3422 0.82 -0.7 246,768 + GFF3422 0.82 +0.3 246,870 + -2.6 246,870 + -0.3 247,047 + GFF3423 0.10 +0.5 247,048 - GFF3423 0.10 +0.1 247,048 - GFF3423 0.10 +1.4 247,203 + GFF3423 0.18 +0.4 247,203 + GFF3423 0.18 -1.0 247,203 + GFF3423 0.18 -1.0 247,204 - GFF3423 0.18 +2.0 247,204 - GFF3423 0.18 -0.2 247,317 + GFF3423 0.24 -0.3 247,317 + GFF3423 0.24 -0.0 247,317 + GFF3423 0.24 -0.5 247,318 - GFF3423 0.24 +0.8 247,803 + GFF3423 0.49 -2.1 247,803 + GFF3423 0.49 -0.2 247,803 + GFF3423 0.49 +0.3 247,803 + GFF3423 0.49 +1.0 247,803 + GFF3423 0.49 +0.1 247,803 + GFF3423 0.49 -0.2 247,803 + GFF3423 0.49 -0.3 247,804 - GFF3423 0.49 -1.0 247,804 - GFF3423 0.49 -0.5 247,818 + GFF3423 0.50 +0.2 247,819 - GFF3423 0.50 +0.3 247,819 - GFF3423 0.50 -0.8
Or see this region's nucleotide sequence