Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3380 and GFF3381 overlap by 4 nucleotides GFF3381 and GFF3382 are separated by 36 nucleotides
GFF3380 - 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35), at 201,021 to 201,968
GFF3380
GFF3381 - Broad-specificity amino acid ABC transporter, substrate-binding protein, at 201,965 to 203,173
GFF3381
GFF3382 - 3-keto-5-aminohexanoate cleavage enzyme (EC 2.3.1.247), at 203,210 to 204,130
GFF3382
Position (kb) 201
202
203
204 Strain fitness (log2 ratio) -2
-1
0
1
2 at 201.023 kb on - strand at 201.023 kb on - strand at 201.023 kb on - strand at 201.023 kb on - strand at 201.063 kb on + strand at 201.454 kb on + strand, within GFF3380 at 201.454 kb on + strand, within GFF3380 at 201.686 kb on + strand, within GFF3380 at 202.047 kb on + strand at 202.047 kb on + strand at 202.047 kb on + strand at 202.047 kb on + strand at 202.048 kb on - strand at 202.048 kb on - strand at 202.048 kb on - strand at 202.048 kb on - strand at 202.048 kb on - strand at 202.048 kb on - strand at 202.050 kb on + strand at 202.050 kb on + strand at 202.051 kb on - strand at 202.233 kb on + strand, within GFF3381 at 202.252 kb on - strand, within GFF3381 at 202.342 kb on - strand, within GFF3381 at 202.359 kb on + strand, within GFF3381 at 202.360 kb on - strand, within GFF3381 at 202.671 kb on + strand, within GFF3381 at 202.671 kb on + strand, within GFF3381 at 202.672 kb on - strand, within GFF3381 at 202.737 kb on + strand, within GFF3381 at 202.738 kb on - strand, within GFF3381 at 202.738 kb on - strand, within GFF3381 at 202.743 kb on + strand, within GFF3381 at 202.743 kb on + strand, within GFF3381 at 202.743 kb on + strand, within GFF3381 at 202.755 kb on + strand, within GFF3381 at 202.756 kb on - strand, within GFF3381 at 202.809 kb on + strand, within GFF3381 at 202.967 kb on + strand, within GFF3381 at 202.967 kb on + strand, within GFF3381 at 203.044 kb on - strand, within GFF3381 at 203.370 kb on + strand, within GFF3382 at 203.370 kb on + strand, within GFF3382 at 203.371 kb on - strand, within GFF3382 at 203.371 kb on - strand, within GFF3382 at 203.371 kb on - strand, within GFF3382 at 203.371 kb on - strand, within GFF3382 at 203.371 kb on - strand, within GFF3382 at 203.371 kb on - strand, within GFF3382 at 203.574 kb on + strand, within GFF3382 at 203.575 kb on - strand, within GFF3382 at 203.575 kb on - strand, within GFF3382 at 203.575 kb on - strand, within GFF3382 at 203.575 kb on - strand, within GFF3382 at 203.937 kb on + strand, within GFF3382 at 203.937 kb on + strand, within GFF3382 at 203.938 kb on - strand, within GFF3382
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 201,023 - -0.5 201,023 - +1.1 201,023 - -0.3 201,023 - -1.2 201,063 + -0.4 201,454 + GFF3380 0.46 -0.4 201,454 + GFF3380 0.46 -1.2 201,686 + GFF3380 0.70 -0.4 202,047 + +1.1 202,047 + +0.2 202,047 + -0.3 202,047 + -0.6 202,048 - -0.3 202,048 - +2.0 202,048 - +0.2 202,048 - -0.4 202,048 - +1.3 202,048 - -0.1 202,050 + -0.4 202,050 + +0.4 202,051 - -0.8 202,233 + GFF3381 0.22 -0.6 202,252 - GFF3381 0.24 -0.0 202,342 - GFF3381 0.31 -1.4 202,359 + GFF3381 0.33 -0.4 202,360 - GFF3381 0.33 -0.6 202,671 + GFF3381 0.58 -0.2 202,671 + GFF3381 0.58 +1.6 202,672 - GFF3381 0.58 +0.4 202,737 + GFF3381 0.64 +1.0 202,738 - GFF3381 0.64 -0.5 202,738 - GFF3381 0.64 +0.3 202,743 + GFF3381 0.64 -0.3 202,743 + GFF3381 0.64 -0.7 202,743 + GFF3381 0.64 -0.5 202,755 + GFF3381 0.65 +0.3 202,756 - GFF3381 0.65 -1.0 202,809 + GFF3381 0.70 +0.0 202,967 + GFF3381 0.83 +0.4 202,967 + GFF3381 0.83 +0.4 203,044 - GFF3381 0.89 +0.6 203,370 + GFF3382 0.17 -0.2 203,370 + GFF3382 0.17 +0.7 203,371 - GFF3382 0.17 -2.3 203,371 - GFF3382 0.17 +1.0 203,371 - GFF3382 0.17 +1.4 203,371 - GFF3382 0.17 +0.2 203,371 - GFF3382 0.17 -0.5 203,371 - GFF3382 0.17 -1.2 203,574 + GFF3382 0.40 -0.3 203,575 - GFF3382 0.40 -0.4 203,575 - GFF3382 0.40 -0.6 203,575 - GFF3382 0.40 +0.3 203,575 - GFF3382 0.40 +0.4 203,937 + GFF3382 0.79 -0.3 203,937 + GFF3382 0.79 +0.0 203,938 - GFF3382 0.79 -0.2
Or see this region's nucleotide sequence