Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3349 and GFF3350 are separated by 3 nucleotides GFF3350 and GFF3351 are separated by 14 nucleotides GFF3351 and GFF3352 overlap by 17 nucleotides
GFF3349 - Branched-chain amino acid ABC transporter, substrate-binding protein LivJ (TC 3.A.1.4.1), at 171,364 to 172,545
GFF3349
GFF3350 - ABC transporter, permease protein 1 (cluster 4, leucine/isoleucine/valine/benzoate) / ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 172,549 to 174,378
GFF3350
GFF3351 - FIG00458971: hypothetical protein, at 174,393 to 174,950
GFF3351
GFF3352 - Branched-chain amino acid ABC transporter, ATP-binding protein LivG (TC 3.A.1.4.1), at 174,934 to 175,776
GFF3352
Position (kb) 172
173
174
175 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 171.601 kb on + strand, within GFF3349 at 171.601 kb on + strand, within GFF3349 at 171.733 kb on + strand, within GFF3349 at 171.766 kb on - strand, within GFF3349 at 171.802 kb on + strand, within GFF3349 at 171.802 kb on + strand, within GFF3349 at 171.802 kb on + strand, within GFF3349 at 171.803 kb on - strand, within GFF3349 at 171.803 kb on - strand, within GFF3349 at 171.899 kb on - strand, within GFF3349 at 171.988 kb on + strand, within GFF3349 at 172.069 kb on + strand, within GFF3349 at 172.070 kb on - strand, within GFF3349 at 172.070 kb on - strand, within GFF3349 at 172.150 kb on + strand, within GFF3349 at 172.151 kb on - strand, within GFF3349 at 172.151 kb on - strand, within GFF3349 at 172.274 kb on - strand, within GFF3349 at 172.274 kb on - strand, within GFF3349 at 172.274 kb on - strand, within GFF3349 at 172.303 kb on + strand, within GFF3349 at 172.325 kb on - strand, within GFF3349 at 172.391 kb on - strand, within GFF3349 at 172.573 kb on + strand at 172.574 kb on - strand at 172.606 kb on + strand at 172.607 kb on - strand at 172.682 kb on - strand at 172.682 kb on - strand at 172.696 kb on + strand at 172.697 kb on - strand at 172.697 kb on - strand at 172.720 kb on + strand at 172.721 kb on - strand at 173.182 kb on + strand, within GFF3350 at 173.182 kb on + strand, within GFF3350 at 173.182 kb on + strand, within GFF3350 at 173.182 kb on + strand, within GFF3350 at 173.182 kb on + strand, within GFF3350 at 173.182 kb on + strand, within GFF3350 at 173.182 kb on + strand, within GFF3350 at 173.182 kb on + strand, within GFF3350 at 173.182 kb on + strand, within GFF3350 at 173.182 kb on + strand, within GFF3350 at 173.182 kb on + strand, within GFF3350 at 173.182 kb on + strand, within GFF3350 at 173.182 kb on + strand, within GFF3350 at 173.182 kb on + strand, within GFF3350 at 173.183 kb on - strand, within GFF3350 at 173.183 kb on - strand, within GFF3350 at 173.183 kb on - strand, within GFF3350 at 173.183 kb on - strand, within GFF3350 at 173.183 kb on - strand, within GFF3350 at 173.183 kb on - strand, within GFF3350 at 173.183 kb on - strand, within GFF3350 at 173.183 kb on - strand, within GFF3350 at 173.183 kb on - strand, within GFF3350 at 173.183 kb on - strand, within GFF3350 at 173.185 kb on + strand, within GFF3350 at 173.185 kb on + strand, within GFF3350 at 173.186 kb on - strand, within GFF3350 at 173.186 kb on - strand, within GFF3350 at 173.315 kb on - strand, within GFF3350 at 173.315 kb on - strand, within GFF3350 at 173.378 kb on + strand, within GFF3350 at 173.378 kb on + strand, within GFF3350 at 173.545 kb on + strand, within GFF3350 at 173.546 kb on - strand, within GFF3350 at 173.546 kb on - strand, within GFF3350 at 173.579 kb on - strand, within GFF3350 at 173.669 kb on - strand, within GFF3350 at 173.794 kb on + strand, within GFF3350 at 173.794 kb on + strand, within GFF3350 at 173.794 kb on + strand, within GFF3350 at 173.794 kb on + strand, within GFF3350 at 173.795 kb on - strand, within GFF3350 at 173.941 kb on + strand, within GFF3350 at 173.941 kb on + strand, within GFF3350 at 173.941 kb on + strand, within GFF3350 at 173.942 kb on - strand, within GFF3350 at 174.079 kb on + strand, within GFF3350 at 174.080 kb on - strand, within GFF3350 at 174.148 kb on + strand, within GFF3350 at 174.148 kb on + strand, within GFF3350 at 174.149 kb on - strand, within GFF3350 at 174.149 kb on - strand, within GFF3350 at 174.149 kb on - strand, within GFF3350 at 174.154 kb on + strand, within GFF3350 at 174.154 kb on + strand, within GFF3350 at 174.155 kb on - strand, within GFF3350 at 174.155 kb on - strand, within GFF3350 at 174.155 kb on - strand, within GFF3350 at 174.317 kb on - strand at 174.336 kb on - strand at 174.411 kb on + strand at 174.412 kb on - strand at 174.412 kb on - strand at 174.585 kb on + strand, within GFF3351 at 174.586 kb on - strand, within GFF3351 at 174.586 kb on - strand, within GFF3351 at 174.586 kb on - strand, within GFF3351 at 174.780 kb on + strand, within GFF3351
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 171,601 + GFF3349 0.20 -0.4 171,601 + GFF3349 0.20 -0.3 171,733 + GFF3349 0.31 +1.2 171,766 - GFF3349 0.34 +0.0 171,802 + GFF3349 0.37 -1.4 171,802 + GFF3349 0.37 -0.7 171,802 + GFF3349 0.37 +0.1 171,803 - GFF3349 0.37 -1.3 171,803 - GFF3349 0.37 -1.0 171,899 - GFF3349 0.45 +0.0 171,988 + GFF3349 0.53 +0.4 172,069 + GFF3349 0.60 +1.1 172,070 - GFF3349 0.60 -1.3 172,070 - GFF3349 0.60 -1.4 172,150 + GFF3349 0.66 -0.2 172,151 - GFF3349 0.67 +0.0 172,151 - GFF3349 0.67 +0.7 172,274 - GFF3349 0.77 +3.4 172,274 - GFF3349 0.77 -0.0 172,274 - GFF3349 0.77 -1.2 172,303 + GFF3349 0.79 +0.6 172,325 - GFF3349 0.81 -2.2 172,391 - GFF3349 0.87 -0.6 172,573 + -0.2 172,574 - +0.1 172,606 + +0.1 172,607 - +0.1 172,682 - -0.3 172,682 - -1.3 172,696 + +0.1 172,697 - -0.3 172,697 - +0.6 172,720 + -0.7 172,721 - -0.2 173,182 + GFF3350 0.35 -1.9 173,182 + GFF3350 0.35 -0.8 173,182 + GFF3350 0.35 -1.3 173,182 + GFF3350 0.35 -0.4 173,182 + GFF3350 0.35 -0.6 173,182 + GFF3350 0.35 +0.0 173,182 + GFF3350 0.35 -0.2 173,182 + GFF3350 0.35 -1.0 173,182 + GFF3350 0.35 -0.2 173,182 + GFF3350 0.35 +0.4 173,182 + GFF3350 0.35 -0.7 173,182 + GFF3350 0.35 -1.6 173,182 + GFF3350 0.35 +0.2 173,182 + GFF3350 0.35 -0.7 173,183 - GFF3350 0.35 -0.3 173,183 - GFF3350 0.35 +0.6 173,183 - GFF3350 0.35 +1.8 173,183 - GFF3350 0.35 -0.6 173,183 - GFF3350 0.35 +0.4 173,183 - GFF3350 0.35 +0.1 173,183 - GFF3350 0.35 -0.6 173,183 - GFF3350 0.35 -0.1 173,183 - GFF3350 0.35 -0.3 173,183 - GFF3350 0.35 +1.4 173,185 + GFF3350 0.35 -0.6 173,185 + GFF3350 0.35 -0.4 173,186 - GFF3350 0.35 -0.8 173,186 - GFF3350 0.35 -0.9 173,315 - GFF3350 0.42 -2.0 173,315 - GFF3350 0.42 -0.5 173,378 + GFF3350 0.45 -0.0 173,378 + GFF3350 0.45 +0.7 173,545 + GFF3350 0.54 -0.6 173,546 - GFF3350 0.54 -1.4 173,546 - GFF3350 0.54 +0.0 173,579 - GFF3350 0.56 -0.1 173,669 - GFF3350 0.61 -0.7 173,794 + GFF3350 0.68 -2.1 173,794 + GFF3350 0.68 +0.3 173,794 + GFF3350 0.68 -0.2 173,794 + GFF3350 0.68 -1.4 173,795 - GFF3350 0.68 -0.6 173,941 + GFF3350 0.76 -0.4 173,941 + GFF3350 0.76 +0.5 173,941 + GFF3350 0.76 -1.0 173,942 - GFF3350 0.76 -1.0 174,079 + GFF3350 0.84 +1.1 174,080 - GFF3350 0.84 +0.4 174,148 + GFF3350 0.87 +0.2 174,148 + GFF3350 0.87 -0.7 174,149 - GFF3350 0.87 -0.8 174,149 - GFF3350 0.87 +1.4 174,149 - GFF3350 0.87 -0.7 174,154 + GFF3350 0.88 -0.4 174,154 + GFF3350 0.88 -0.4 174,155 - GFF3350 0.88 +0.2 174,155 - GFF3350 0.88 -1.3 174,155 - GFF3350 0.88 -0.3 174,317 - -0.2 174,336 - -0.0 174,411 + -1.6 174,412 - -0.2 174,412 - -1.8 174,585 + GFF3351 0.34 -0.2 174,586 - GFF3351 0.35 +1.3 174,586 - GFF3351 0.35 +0.0 174,586 - GFF3351 0.35 +0.2 174,780 + GFF3351 0.69 +0.0
Or see this region's nucleotide sequence