Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3112 and GFF3113 are separated by 34 nucleotides GFF3113 and GFF3114 overlap by 8 nucleotides GFF3114 and GFF3115 are separated by 237 nucleotides
GFF3112 - Methionine ABC transporter substrate-binding protein, at 539,047 to 539,883
GFF3112
GFF3113 - Methionine ABC transporter permease protein, at 539,918 to 540,580
GFF3113
GFF3114 - Methionine ABC transporter ATP-binding protein, at 540,573 to 541,460
GFF3114
GFF3115 - Methionine ABC transporter substrate-binding protein, at 541,698 to 542,513
GFF3115
Position (kb) 540
541
542 Strain fitness (log2 ratio) -2
-1
0
1
2 at 539.591 kb on + strand, within GFF3112 at 539.592 kb on - strand, within GFF3112 at 539.657 kb on + strand, within GFF3112 at 539.658 kb on - strand, within GFF3112 at 539.658 kb on - strand, within GFF3112 at 539.658 kb on - strand, within GFF3112 at 539.687 kb on + strand, within GFF3112 at 539.687 kb on + strand, within GFF3112 at 539.687 kb on + strand, within GFF3112 at 539.687 kb on + strand, within GFF3112 at 539.688 kb on - strand, within GFF3112 at 539.688 kb on - strand, within GFF3112 at 539.688 kb on - strand, within GFF3112 at 539.898 kb on - strand at 539.898 kb on - strand at 539.898 kb on - strand at 539.898 kb on - strand at 539.901 kb on + strand at 539.901 kb on + strand at 539.901 kb on + strand at 539.901 kb on + strand at 539.902 kb on - strand at 539.902 kb on - strand at 539.902 kb on - strand at 539.902 kb on - strand at 539.972 kb on + strand at 539.972 kb on + strand at 539.973 kb on - strand at 539.973 kb on - strand at 540.027 kb on + strand, within GFF3113 at 540.027 kb on + strand, within GFF3113 at 540.028 kb on - strand, within GFF3113 at 540.028 kb on - strand, within GFF3113 at 540.030 kb on + strand, within GFF3113 at 540.031 kb on - strand, within GFF3113 at 540.093 kb on + strand, within GFF3113 at 540.093 kb on + strand, within GFF3113 at 540.094 kb on - strand, within GFF3113 at 540.264 kb on + strand, within GFF3113 at 540.264 kb on + strand, within GFF3113 at 540.265 kb on - strand, within GFF3113 at 540.265 kb on - strand, within GFF3113 at 540.265 kb on - strand, within GFF3113 at 540.580 kb on + strand at 540.580 kb on + strand at 540.581 kb on - strand at 540.581 kb on - strand at 541.000 kb on + strand, within GFF3114 at 541.000 kb on + strand, within GFF3114 at 541.521 kb on + strand at 541.522 kb on - strand at 541.608 kb on - strand at 541.623 kb on - strand at 541.628 kb on + strand at 541.628 kb on + strand at 541.628 kb on + strand at 541.628 kb on + strand at 541.628 kb on + strand at 541.628 kb on + strand at 541.628 kb on + strand at 541.629 kb on - strand at 541.629 kb on - strand at 541.629 kb on - strand at 541.629 kb on - strand at 541.629 kb on - strand at 541.629 kb on - strand at 541.902 kb on + strand, within GFF3115 at 541.902 kb on + strand, within GFF3115 at 541.902 kb on + strand, within GFF3115 at 541.903 kb on - strand, within GFF3115 at 541.903 kb on - strand, within GFF3115 at 542.046 kb on + strand, within GFF3115 at 542.046 kb on + strand, within GFF3115 at 542.046 kb on + strand, within GFF3115 at 542.046 kb on + strand, within GFF3115 at 542.047 kb on - strand, within GFF3115 at 542.047 kb on - strand, within GFF3115 at 542.047 kb on - strand, within GFF3115 at 542.047 kb on - strand, within GFF3115 at 542.376 kb on + strand, within GFF3115
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 539,591 + GFF3112 0.65 -0.0 539,592 - GFF3112 0.65 -1.4 539,657 + GFF3112 0.73 +0.8 539,658 - GFF3112 0.73 -0.4 539,658 - GFF3112 0.73 +0.2 539,658 - GFF3112 0.73 -0.0 539,687 + GFF3112 0.76 +0.4 539,687 + GFF3112 0.76 +0.2 539,687 + GFF3112 0.76 +0.2 539,687 + GFF3112 0.76 +1.2 539,688 - GFF3112 0.77 +0.0 539,688 - GFF3112 0.77 +0.9 539,688 - GFF3112 0.77 -0.5 539,898 - +1.0 539,898 - -1.7 539,898 - -0.2 539,898 - -0.2 539,901 + +0.5 539,901 + +0.7 539,901 + -0.2 539,901 + -0.5 539,902 - -0.8 539,902 - -0.4 539,902 - +1.1 539,902 - -0.8 539,972 + -0.9 539,972 + +0.2 539,973 - -0.7 539,973 - +1.4 540,027 + GFF3113 0.16 +1.2 540,027 + GFF3113 0.16 -2.0 540,028 - GFF3113 0.17 -0.2 540,028 - GFF3113 0.17 -0.6 540,030 + GFF3113 0.17 -0.6 540,031 - GFF3113 0.17 +0.1 540,093 + GFF3113 0.26 -0.7 540,093 + GFF3113 0.26 +0.8 540,094 - GFF3113 0.27 +0.2 540,264 + GFF3113 0.52 -0.2 540,264 + GFF3113 0.52 +0.8 540,265 - GFF3113 0.52 -0.6 540,265 - GFF3113 0.52 -0.6 540,265 - GFF3113 0.52 -0.7 540,580 + +0.3 540,580 + -1.9 540,581 - +0.2 540,581 - +0.1 541,000 + GFF3114 0.48 +0.9 541,000 + GFF3114 0.48 -0.0 541,521 + +0.3 541,522 - +0.4 541,608 - +0.3 541,623 - -1.3 541,628 + +0.3 541,628 + +1.8 541,628 + +0.2 541,628 + -0.0 541,628 + -0.5 541,628 + -0.3 541,628 + -0.0 541,629 - -0.5 541,629 - -0.3 541,629 - +0.2 541,629 - -0.3 541,629 - +0.8 541,629 - -0.4 541,902 + GFF3115 0.25 -0.6 541,902 + GFF3115 0.25 -0.5 541,902 + GFF3115 0.25 +2.1 541,903 - GFF3115 0.25 +1.8 541,903 - GFF3115 0.25 -1.4 542,046 + GFF3115 0.43 -0.6 542,046 + GFF3115 0.43 +1.8 542,046 + GFF3115 0.43 +0.1 542,046 + GFF3115 0.43 -0.1 542,047 - GFF3115 0.43 -0.2 542,047 - GFF3115 0.43 -0.6 542,047 - GFF3115 0.43 -1.5 542,047 - GFF3115 0.43 -1.2 542,376 + GFF3115 0.83 -0.8
Or see this region's nucleotide sequence