Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3035 and GFF3036 are separated by 9 nucleotides GFF3036 and GFF3037 are separated by 6 nucleotides GFF3037 and GFF3038 are separated by 11 nucleotides GFF3038 and GFF3039 are separated by 250 nucleotides
GFF3035 - Ferrichrome-iron receptor, at 467,378 to 469,573
GFF3035
GFF3036 - no description, at 469,583 to 470,005
GFF3036
GFF3037 - no description, at 470,012 to 470,479
GFF3037
GFF3038 - hypothetical protein, at 470,491 to 471,174
GFF3038
GFF3039 - Two-component transcriptional response regulator, LuxR family, at 471,425 to 472,204
GFF3039
Position (kb)
470
471
472 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 469.517 kb on + strand at 469.517 kb on + strand at 469.571 kb on + strand at 469.710 kb on - strand, within GFF3036 at 469.721 kb on + strand, within GFF3036 at 469.743 kb on - strand, within GFF3036 at 469.743 kb on - strand, within GFF3036 at 469.743 kb on - strand, within GFF3036 at 469.895 kb on + strand, within GFF3036 at 469.896 kb on - strand, within GFF3036 at 470.351 kb on + strand, within GFF3037 at 470.351 kb on + strand, within GFF3037 at 470.351 kb on + strand, within GFF3037 at 470.352 kb on - strand, within GFF3037 at 470.812 kb on + strand, within GFF3038 at 470.848 kb on + strand, within GFF3038 at 470.950 kb on - strand, within GFF3038 at 470.950 kb on - strand, within GFF3038 at 470.950 kb on - strand, within GFF3038 at 470.988 kb on - strand, within GFF3038 at 471.465 kb on + strand at 471.466 kb on - strand at 471.466 kb on - strand at 471.466 kb on - strand at 471.741 kb on + strand, within GFF3039 at 471.876 kb on + strand, within GFF3039 at 471.876 kb on + strand, within GFF3039 at 471.877 kb on - strand, within GFF3039 at 471.877 kb on - strand, within GFF3039 at 472.032 kb on + strand, within GFF3039
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 469,517 + -0.1 469,517 + +0.6 469,571 + -0.3 469,710 - GFF3036 0.30 +0.5 469,721 + GFF3036 0.33 -0.2 469,743 - GFF3036 0.38 -1.7 469,743 - GFF3036 0.38 -0.2 469,743 - GFF3036 0.38 +1.5 469,895 + GFF3036 0.74 -0.7 469,896 - GFF3036 0.74 +0.3 470,351 + GFF3037 0.72 -0.4 470,351 + GFF3037 0.72 -0.3 470,351 + GFF3037 0.72 +0.2 470,352 - GFF3037 0.73 -1.1 470,812 + GFF3038 0.47 -0.6 470,848 + GFF3038 0.52 +0.2 470,950 - GFF3038 0.67 +0.9 470,950 - GFF3038 0.67 +0.5 470,950 - GFF3038 0.67 +0.3 470,988 - GFF3038 0.73 +0.5 471,465 + +1.5 471,466 - -0.5 471,466 - -0.6 471,466 - -0.1 471,741 + GFF3039 0.41 -1.0 471,876 + GFF3039 0.58 -0.2 471,876 + GFF3039 0.58 -1.8 471,877 - GFF3039 0.58 +1.8 471,877 - GFF3039 0.58 +0.8 472,032 + GFF3039 0.78 +0.3
Or see this region's nucleotide sequence