Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2965 and GFF2966 are separated by 4 nucleotides GFF2966 and GFF2967 overlap by 8 nucleotides GFF2967 and GFF2968 are separated by 36 nucleotides GFF2968 and GFF2969 are separated by 11 nucleotides
GFF2965 - Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 396,382 to 397,578
GFF2965
GFF2966 - Enoyl-CoA hydratase (EC 4.2.1.17), at 397,583 to 398,395
GFF2966
GFF2967 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 398,388 to 399,137
GFF2967
GFF2968 - MaoC-like dehydratase, at 399,174 to 400,043
GFF2968
GFF2969 - acyl-CoA synthetase (NDP forming type), at 400,055 to 402,211
GFF2969
Position (kb)
398
399
400 Strain fitness (log2 ratio)
-2
-1
0
1 at 397.409 kb on + strand, within GFF2965 at 397.410 kb on - strand, within GFF2965 at 397.410 kb on - strand, within GFF2965 at 397.410 kb on - strand, within GFF2965 at 397.410 kb on - strand, within GFF2965 at 397.805 kb on + strand, within GFF2966 at 398.395 kb on + strand at 398.395 kb on + strand at 398.396 kb on - strand at 398.396 kb on - strand at 398.396 kb on - strand at 398.542 kb on + strand, within GFF2967 at 398.542 kb on + strand, within GFF2967 at 398.542 kb on + strand, within GFF2967 at 398.542 kb on + strand, within GFF2967 at 398.542 kb on + strand, within GFF2967 at 398.543 kb on - strand, within GFF2967 at 398.543 kb on - strand, within GFF2967 at 398.674 kb on + strand, within GFF2967 at 398.675 kb on - strand, within GFF2967 at 398.675 kb on - strand, within GFF2967 at 398.675 kb on - strand, within GFF2967 at 398.675 kb on - strand, within GFF2967 at 398.675 kb on - strand, within GFF2967 at 398.675 kb on - strand, within GFF2967 at 398.880 kb on - strand, within GFF2967 at 398.880 kb on - strand, within GFF2967 at 398.880 kb on - strand, within GFF2967 at 398.880 kb on - strand, within GFF2967 at 399.344 kb on - strand, within GFF2968 at 399.482 kb on - strand, within GFF2968 at 399.901 kb on + strand, within GFF2968 at 399.902 kb on - strand, within GFF2968 at 399.907 kb on + strand, within GFF2968 at 399.907 kb on + strand, within GFF2968 at 399.907 kb on + strand, within GFF2968 at 399.907 kb on + strand, within GFF2968 at 399.908 kb on - strand, within GFF2968 at 399.908 kb on - strand, within GFF2968 at 399.908 kb on - strand, within GFF2968 at 399.961 kb on + strand at 399.961 kb on + strand at 399.961 kb on + strand at 399.962 kb on - strand at 399.962 kb on - strand at 399.983 kb on + strand at 399.985 kb on + strand at 399.985 kb on + strand at 399.985 kb on + strand at 399.985 kb on + strand at 399.985 kb on + strand at 399.985 kb on + strand at 399.985 kb on + strand at 399.985 kb on + strand at 399.986 kb on - strand at 399.986 kb on - strand at 399.986 kb on - strand at 399.986 kb on - strand at 399.986 kb on - strand at 399.986 kb on - strand at 399.986 kb on - strand at 400.038 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 397,409 + GFF2965 0.86 -0.1 397,410 - GFF2965 0.86 +0.5 397,410 - GFF2965 0.86 -1.5 397,410 - GFF2965 0.86 -1.2 397,410 - GFF2965 0.86 -0.3 397,805 + GFF2966 0.27 -1.3 398,395 + -0.4 398,395 + -0.5 398,396 - +0.2 398,396 - -0.2 398,396 - +0.6 398,542 + GFF2967 0.21 -0.4 398,542 + GFF2967 0.21 -1.6 398,542 + GFF2967 0.21 +0.8 398,542 + GFF2967 0.21 +0.4 398,542 + GFF2967 0.21 +0.5 398,543 - GFF2967 0.21 -0.8 398,543 - GFF2967 0.21 +0.3 398,674 + GFF2967 0.38 +0.6 398,675 - GFF2967 0.38 +0.2 398,675 - GFF2967 0.38 -0.8 398,675 - GFF2967 0.38 +1.0 398,675 - GFF2967 0.38 +1.2 398,675 - GFF2967 0.38 +1.0 398,675 - GFF2967 0.38 -0.2 398,880 - GFF2967 0.66 -0.2 398,880 - GFF2967 0.66 -1.0 398,880 - GFF2967 0.66 -0.6 398,880 - GFF2967 0.66 +1.2 399,344 - GFF2968 0.20 -0.4 399,482 - GFF2968 0.35 +1.2 399,901 + GFF2968 0.84 +0.1 399,902 - GFF2968 0.84 -1.6 399,907 + GFF2968 0.84 -0.1 399,907 + GFF2968 0.84 -0.6 399,907 + GFF2968 0.84 +0.6 399,907 + GFF2968 0.84 +0.4 399,908 - GFF2968 0.84 -1.1 399,908 - GFF2968 0.84 -0.3 399,908 - GFF2968 0.84 -0.0 399,961 + +0.5 399,961 + +0.5 399,961 + -1.1 399,962 - +0.3 399,962 - -1.1 399,983 + -0.1 399,985 + +0.4 399,985 + +0.4 399,985 + -0.2 399,985 + -0.1 399,985 + -1.0 399,985 + +0.4 399,985 + +0.1 399,985 + -0.2 399,986 - +0.8 399,986 - -0.8 399,986 - -0.2 399,986 - +0.1 399,986 - -0.7 399,986 - -1.8 399,986 - -2.2 400,038 - +1.3
Or see this region's nucleotide sequence