Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2905 and GFF2906 are separated by 767 nucleotides GFF2906 and GFF2907 are separated by 13 nucleotides
GFF2905 - surface antigen gene, at 330,911 to 332,317
GFF2905
GFF2906 - 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26), at 333,085 to 334,521
GFF2906
GFF2907 - Glucarate dehydratase (EC 4.2.1.40), at 334,535 to 335,893
GFF2907
Position (kb) 333
334
335 Strain fitness (log2 ratio) -2
-1
0
1
2 at 332.183 kb on + strand at 332.184 kb on - strand at 332.583 kb on + strand at 332.584 kb on - strand at 332.584 kb on - strand at 332.585 kb on + strand at 332.585 kb on + strand at 332.586 kb on - strand at 332.586 kb on - strand at 332.586 kb on - strand at 332.657 kb on - strand at 332.703 kb on + strand at 332.703 kb on + strand at 332.703 kb on + strand at 332.703 kb on + strand at 332.703 kb on + strand at 332.751 kb on + strand at 332.751 kb on + strand at 332.751 kb on + strand at 332.817 kb on + strand at 332.818 kb on - strand at 332.880 kb on + strand at 332.880 kb on + strand at 332.880 kb on + strand at 332.881 kb on - strand at 332.881 kb on - strand at 332.881 kb on - strand at 332.960 kb on - strand at 333.003 kb on - strand at 333.003 kb on - strand at 333.003 kb on - strand at 333.098 kb on + strand at 333.099 kb on - strand at 333.114 kb on + strand at 333.114 kb on + strand at 333.115 kb on - strand at 333.135 kb on - strand at 333.158 kb on + strand at 333.158 kb on + strand at 333.159 kb on - strand at 333.159 kb on - strand at 333.194 kb on + strand at 333.194 kb on + strand at 333.195 kb on - strand at 333.195 kb on - strand at 333.195 kb on - strand at 333.266 kb on + strand, within GFF2906 at 333.267 kb on - strand, within GFF2906 at 333.267 kb on - strand, within GFF2906 at 333.267 kb on - strand, within GFF2906 at 333.267 kb on - strand, within GFF2906 at 333.440 kb on + strand, within GFF2906 at 333.440 kb on + strand, within GFF2906 at 333.440 kb on + strand, within GFF2906 at 333.440 kb on + strand, within GFF2906 at 333.441 kb on - strand, within GFF2906 at 333.441 kb on - strand, within GFF2906 at 333.441 kb on - strand, within GFF2906 at 333.441 kb on - strand, within GFF2906 at 333.441 kb on - strand, within GFF2906 at 333.441 kb on - strand, within GFF2906 at 333.441 kb on - strand, within GFF2906 at 333.441 kb on - strand, within GFF2906 at 333.518 kb on + strand, within GFF2906 at 333.518 kb on + strand, within GFF2906 at 333.519 kb on - strand, within GFF2906 at 333.519 kb on - strand, within GFF2906 at 333.642 kb on - strand, within GFF2906 at 333.698 kb on + strand, within GFF2906 at 333.698 kb on + strand, within GFF2906 at 333.699 kb on - strand, within GFF2906 at 333.699 kb on - strand, within GFF2906 at 334.421 kb on + strand at 334.469 kb on + strand at 334.469 kb on + strand at 334.470 kb on - strand at 334.470 kb on - strand at 334.470 kb on - strand at 334.470 kb on - strand at 334.470 kb on - strand at 334.587 kb on + strand at 334.636 kb on - strand at 334.636 kb on - strand at 335.100 kb on + strand, within GFF2907 at 335.101 kb on - strand, within GFF2907 at 335.382 kb on + strand, within GFF2907 at 335.382 kb on + strand, within GFF2907 at 335.415 kb on + strand, within GFF2907 at 335.415 kb on + strand, within GFF2907 at 335.416 kb on - strand, within GFF2907 at 335.438 kb on + strand, within GFF2907
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 332,183 + +0.9 332,184 - -0.2 332,583 + -1.3 332,584 - -0.4 332,584 - +2.4 332,585 + -0.2 332,585 + -2.1 332,586 - +2.0 332,586 - +0.3 332,586 - +0.7 332,657 - +0.1 332,703 + -0.7 332,703 + -1.4 332,703 + -0.9 332,703 + -0.8 332,703 + -0.3 332,751 + +0.1 332,751 + -0.9 332,751 + -2.0 332,817 + -1.0 332,818 - +1.0 332,880 + +1.0 332,880 + +0.8 332,880 + -0.6 332,881 - -1.3 332,881 - +0.4 332,881 - -1.6 332,960 - +0.1 333,003 - -0.9 333,003 - -0.4 333,003 - -0.2 333,098 + +0.4 333,099 - +1.2 333,114 + -1.4 333,114 + +1.0 333,115 - -0.4 333,135 - +0.0 333,158 + -0.6 333,158 + -0.7 333,159 - +0.4 333,159 - -0.6 333,194 + -0.6 333,194 + -0.1 333,195 - +0.7 333,195 - -0.7 333,195 - +0.3 333,266 + GFF2906 0.13 -0.5 333,267 - GFF2906 0.13 -0.0 333,267 - GFF2906 0.13 +0.5 333,267 - GFF2906 0.13 +0.1 333,267 - GFF2906 0.13 -0.0 333,440 + GFF2906 0.25 +0.7 333,440 + GFF2906 0.25 +0.8 333,440 + GFF2906 0.25 +0.1 333,440 + GFF2906 0.25 -1.5 333,441 - GFF2906 0.25 +1.3 333,441 - GFF2906 0.25 +1.3 333,441 - GFF2906 0.25 -0.0 333,441 - GFF2906 0.25 +0.4 333,441 - GFF2906 0.25 +0.1 333,441 - GFF2906 0.25 -0.5 333,441 - GFF2906 0.25 +0.1 333,441 - GFF2906 0.25 +0.1 333,518 + GFF2906 0.30 -0.1 333,518 + GFF2906 0.30 +0.7 333,519 - GFF2906 0.30 -0.4 333,519 - GFF2906 0.30 +1.7 333,642 - GFF2906 0.39 -0.3 333,698 + GFF2906 0.43 -1.3 333,698 + GFF2906 0.43 -0.2 333,699 - GFF2906 0.43 -0.4 333,699 - GFF2906 0.43 -0.4 334,421 + -0.3 334,469 + -0.0 334,469 + +0.9 334,470 - -0.3 334,470 - +0.1 334,470 - +0.3 334,470 - -0.4 334,470 - -0.4 334,587 + -0.6 334,636 - +1.1 334,636 - -1.3 335,100 + GFF2907 0.42 -0.4 335,101 - GFF2907 0.42 -0.3 335,382 + GFF2907 0.62 -0.1 335,382 + GFF2907 0.62 -0.1 335,415 + GFF2907 0.65 -0.0 335,415 + GFF2907 0.65 +1.1 335,416 - GFF2907 0.65 +1.3 335,438 + GFF2907 0.66 +0.9
Or see this region's nucleotide sequence