Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2819 and GFF2820 are separated by 49 nucleotides GFF2820 and GFF2821 overlap by 4 nucleotides GFF2821 and GFF2822 overlap by 4 nucleotides GFF2822 and GFF2823 overlap by 4 nucleotides
GFF2819 - Phosphoenolpyruvate synthase regulatory protein, at 246,024 to 246,851
GFF2819
GFF2820 - ABC-type hemin transport system, ATPase component, at 246,901 to 247,707
GFF2820
GFF2821 - Hemin ABC transporter, permease protein, at 247,704 to 248,738
GFF2821
GFF2822 - Periplasmic hemin-binding protein, at 248,735 to 249,634
GFF2822
GFF2823 - Hemin transport protein HmuS, at 249,631 to 250,731
GFF2823
Position (kb) 247
248
249 Strain fitness (log2 ratio) -2
-1
0
1 at 246.846 kb on + strand at 246.846 kb on + strand at 246.847 kb on - strand at 246.914 kb on + strand at 246.914 kb on + strand at 246.915 kb on - strand at 246.915 kb on - strand at 246.915 kb on - strand at 246.915 kb on - strand at 246.915 kb on - strand at 246.915 kb on - strand at 247.045 kb on + strand, within GFF2820 at 247.045 kb on + strand, within GFF2820 at 247.045 kb on + strand, within GFF2820 at 247.045 kb on + strand, within GFF2820 at 247.046 kb on - strand, within GFF2820 at 247.046 kb on - strand, within GFF2820 at 247.046 kb on - strand, within GFF2820 at 247.046 kb on - strand, within GFF2820 at 247.046 kb on - strand, within GFF2820 at 247.046 kb on - strand, within GFF2820 at 247.046 kb on - strand, within GFF2820 at 247.059 kb on - strand, within GFF2820 at 247.248 kb on + strand, within GFF2820 at 247.249 kb on - strand, within GFF2820 at 247.249 kb on - strand, within GFF2820 at 247.379 kb on + strand, within GFF2820 at 247.380 kb on - strand, within GFF2820 at 247.644 kb on + strand at 247.644 kb on + strand at 247.707 kb on + strand at 247.707 kb on + strand at 247.707 kb on + strand at 247.707 kb on + strand at 247.707 kb on + strand at 247.707 kb on + strand at 247.708 kb on - strand at 247.708 kb on - strand at 247.708 kb on - strand at 247.708 kb on - strand at 247.708 kb on - strand at 247.708 kb on - strand at 247.708 kb on - strand at 247.708 kb on - strand at 247.708 kb on - strand at 247.708 kb on - strand at 247.708 kb on - strand at 247.708 kb on - strand at 247.708 kb on - strand at 247.708 kb on - strand at 247.817 kb on + strand, within GFF2821 at 248.302 kb on + strand, within GFF2821 at 248.302 kb on + strand, within GFF2821 at 248.302 kb on + strand, within GFF2821 at 248.303 kb on - strand, within GFF2821 at 248.303 kb on - strand, within GFF2821 at 248.303 kb on - strand, within GFF2821 at 248.303 kb on - strand, within GFF2821 at 248.647 kb on + strand at 248.647 kb on + strand at 248.647 kb on + strand at 248.647 kb on + strand at 248.647 kb on + strand at 248.647 kb on + strand at 248.647 kb on + strand at 248.648 kb on - strand at 248.648 kb on - strand at 248.648 kb on - strand at 248.648 kb on - strand at 249.019 kb on - strand, within GFF2822 at 249.381 kb on + strand, within GFF2822 at 249.381 kb on + strand, within GFF2822 at 249.381 kb on + strand, within GFF2822 at 249.381 kb on + strand, within GFF2822 at 249.381 kb on + strand, within GFF2822 at 249.381 kb on + strand, within GFF2822 at 249.381 kb on + strand, within GFF2822 at 249.381 kb on + strand, within GFF2822 at 249.381 kb on + strand, within GFF2822 at 249.382 kb on - strand, within GFF2822 at 249.382 kb on - strand, within GFF2822 at 249.382 kb on - strand, within GFF2822 at 249.382 kb on - strand, within GFF2822 at 249.418 kb on - strand, within GFF2822 at 249.468 kb on + strand, within GFF2822 at 249.468 kb on + strand, within GFF2822 at 249.468 kb on + strand, within GFF2822 at 249.468 kb on + strand, within GFF2822 at 249.469 kb on - strand, within GFF2822 at 249.469 kb on - strand, within GFF2822 at 249.469 kb on - strand, within GFF2822 at 249.469 kb on - strand, within GFF2822 at 249.469 kb on - strand, within GFF2822 at 249.469 kb on - strand, within GFF2822 at 249.584 kb on + strand at 249.584 kb on + strand at 249.584 kb on + strand at 249.584 kb on + strand at 249.584 kb on + strand at 249.585 kb on - strand at 249.585 kb on - strand at 249.585 kb on - strand at 249.585 kb on - strand at 249.585 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 246,846 + -0.3 246,846 + +0.4 246,847 - +0.4 246,914 + -0.4 246,914 + -0.3 246,915 - +0.1 246,915 - +1.4 246,915 - -0.2 246,915 - +0.3 246,915 - -0.1 246,915 - +0.3 247,045 + GFF2820 0.18 +0.3 247,045 + GFF2820 0.18 -0.0 247,045 + GFF2820 0.18 +1.7 247,045 + GFF2820 0.18 -0.7 247,046 - GFF2820 0.18 +0.1 247,046 - GFF2820 0.18 -1.2 247,046 - GFF2820 0.18 -1.1 247,046 - GFF2820 0.18 -0.1 247,046 - GFF2820 0.18 -0.8 247,046 - GFF2820 0.18 -0.9 247,046 - GFF2820 0.18 -0.3 247,059 - GFF2820 0.20 -1.6 247,248 + GFF2820 0.43 +0.2 247,249 - GFF2820 0.43 -0.7 247,249 - GFF2820 0.43 +0.1 247,379 + GFF2820 0.59 -0.7 247,380 - GFF2820 0.59 -0.7 247,644 + -0.2 247,644 + +0.1 247,707 + -0.3 247,707 + -1.2 247,707 + -1.7 247,707 + -0.2 247,707 + -1.3 247,707 + +0.1 247,708 - +0.0 247,708 - +0.7 247,708 - +0.7 247,708 - -0.2 247,708 - -0.3 247,708 - -1.5 247,708 - +1.0 247,708 - +0.2 247,708 - -1.3 247,708 - -0.5 247,708 - -0.6 247,708 - +0.4 247,708 - -0.9 247,708 - +0.6 247,817 + GFF2821 0.11 -1.3 248,302 + GFF2821 0.58 +0.9 248,302 + GFF2821 0.58 -1.6 248,302 + GFF2821 0.58 -0.6 248,303 - GFF2821 0.58 -2.3 248,303 - GFF2821 0.58 +0.1 248,303 - GFF2821 0.58 -0.7 248,303 - GFF2821 0.58 -0.3 248,647 + -0.2 248,647 + +1.7 248,647 + -0.0 248,647 + +0.3 248,647 + +0.2 248,647 + -1.2 248,647 + -1.7 248,648 - -0.7 248,648 - -0.7 248,648 - +0.8 248,648 - -0.2 249,019 - GFF2822 0.32 +0.7 249,381 + GFF2822 0.72 +0.0 249,381 + GFF2822 0.72 +1.1 249,381 + GFF2822 0.72 -0.6 249,381 + GFF2822 0.72 -0.6 249,381 + GFF2822 0.72 -0.9 249,381 + GFF2822 0.72 -0.4 249,381 + GFF2822 0.72 -0.2 249,381 + GFF2822 0.72 -0.5 249,381 + GFF2822 0.72 +0.1 249,382 - GFF2822 0.72 +0.5 249,382 - GFF2822 0.72 -1.1 249,382 - GFF2822 0.72 -1.9 249,382 - GFF2822 0.72 +1.1 249,418 - GFF2822 0.76 +0.7 249,468 + GFF2822 0.81 -0.6 249,468 + GFF2822 0.81 -0.7 249,468 + GFF2822 0.81 -0.3 249,468 + GFF2822 0.81 +0.2 249,469 - GFF2822 0.82 -0.0 249,469 - GFF2822 0.82 +1.0 249,469 - GFF2822 0.82 -0.4 249,469 - GFF2822 0.82 +0.2 249,469 - GFF2822 0.82 -0.6 249,469 - GFF2822 0.82 -0.8 249,584 + +0.7 249,584 + -0.4 249,584 + +0.1 249,584 + -0.4 249,584 + -0.5 249,585 - -0.7 249,585 - +0.7 249,585 - -0.2 249,585 - +0.2 249,585 - -0.5
Or see this region's nucleotide sequence