Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2696 and GFF2697 overlap by 1 nucleotides GFF2697 and GFF2698 are separated by 49 nucleotides GFF2698 and GFF2699 are separated by 27 nucleotides GFF2699 and GFF2700 are separated by 28 nucleotides
GFF2696 - L-2-keto-3-deoxyarabonate dehydratase (EC 4.2.1.43), at 124,563 to 125,501
GFF2696
GFF2697 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 125,501 to 126,262
GFF2697
GFF2698 - Transcriptional regulator, LysR family, at 126,312 to 127,226
GFF2698
GFF2699 - 4Fe-4S ferredoxin, iron-sulfur binding, at 127,254 to 127,589
GFF2699
GFF2700 - Enoyl-CoA hydratase (EC 4.2.1.17), at 127,618 to 128,394
GFF2700
Position (kb)
126
127
128 Strain fitness (log2 ratio)
-2
-1
0
1 at 125.791 kb on - strand, within GFF2697 at 125.791 kb on - strand, within GFF2697 at 125.940 kb on + strand, within GFF2697 at 125.940 kb on + strand, within GFF2697 at 125.941 kb on - strand, within GFF2697 at 126.862 kb on + strand, within GFF2698 at 126.862 kb on + strand, within GFF2698 at 126.862 kb on + strand, within GFF2698 at 126.863 kb on - strand, within GFF2698 at 126.863 kb on - strand, within GFF2698 at 127.204 kb on + strand at 127.423 kb on + strand, within GFF2699 at 127.489 kb on + strand, within GFF2699 at 127.511 kb on - strand, within GFF2699 at 127.511 kb on - strand, within GFF2699 at 127.597 kb on - strand at 127.620 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 125,791 - GFF2697 0.38 +1.7 125,791 - GFF2697 0.38 -1.0 125,940 + GFF2697 0.58 -1.5 125,940 + GFF2697 0.58 +0.3 125,941 - GFF2697 0.58 -1.3 126,862 + GFF2698 0.60 -1.9 126,862 + GFF2698 0.60 -1.9 126,862 + GFF2698 0.60 -2.0 126,863 - GFF2698 0.60 +1.4 126,863 - GFF2698 0.60 +0.4 127,204 + -0.8 127,423 + GFF2699 0.50 +0.1 127,489 + GFF2699 0.70 +0.1 127,511 - GFF2699 0.76 -0.9 127,511 - GFF2699 0.76 -1.2 127,597 - -0.3 127,620 - +0.4
Or see this region's nucleotide sequence