Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2221 and GFF2222 are separated by 16 nucleotides GFF2222 and GFF2223 are separated by 139 nucleotides GFF2223 and GFF2224 overlap by 8 nucleotides
GFF2221 - ABC transporter, substrate-binding protein (cluster 4, leucine/isoleucine/valine/benzoate), at 283,180 to 284,433
GFF2221
GFF2222 - ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 284,450 to 285,169
GFF2222
GFF2223 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 285,309 to 286,025
GFF2223
GFF2224 - ABC transporter, permease protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 286,018 to 286,884
GFF2224
Position (kb) 284
285
286 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 283.633 kb on + strand, within GFF2221 at 283.633 kb on + strand, within GFF2221 at 283.633 kb on + strand, within GFF2221 at 283.633 kb on + strand, within GFF2221 at 283.633 kb on + strand, within GFF2221 at 283.634 kb on - strand, within GFF2221 at 283.634 kb on - strand, within GFF2221 at 283.634 kb on - strand, within GFF2221 at 284.356 kb on + strand at 284.487 kb on - strand at 284.634 kb on - strand, within GFF2222 at 284.699 kb on + strand, within GFF2222 at 284.700 kb on - strand, within GFF2222 at 284.700 kb on - strand, within GFF2222 at 285.149 kb on + strand at 285.150 kb on - strand at 285.349 kb on - strand at 285.469 kb on + strand, within GFF2223 at 285.828 kb on + strand, within GFF2223 at 285.829 kb on - strand, within GFF2223 at 285.868 kb on + strand, within GFF2223 at 285.921 kb on + strand, within GFF2223 at 285.922 kb on - strand, within GFF2223 at 285.922 kb on - strand, within GFF2223 at 285.922 kb on - strand, within GFF2223 at 285.922 kb on - strand, within GFF2223 at 285.922 kb on - strand, within GFF2223 at 285.922 kb on - strand, within GFF2223 at 285.922 kb on - strand, within GFF2223 at 285.982 kb on + strand at 285.996 kb on + strand at 285.996 kb on + strand at 285.997 kb on - strand at 286.060 kb on + strand at 286.060 kb on + strand at 286.061 kb on - strand at 286.061 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 283,633 + GFF2221 0.36 +0.5 283,633 + GFF2221 0.36 -1.8 283,633 + GFF2221 0.36 -0.3 283,633 + GFF2221 0.36 +0.2 283,633 + GFF2221 0.36 -0.2 283,634 - GFF2221 0.36 -1.7 283,634 - GFF2221 0.36 -0.3 283,634 - GFF2221 0.36 -0.6 284,356 + +0.3 284,487 - -0.6 284,634 - GFF2222 0.26 -1.1 284,699 + GFF2222 0.35 +1.0 284,700 - GFF2222 0.35 -1.2 284,700 - GFF2222 0.35 -1.6 285,149 + +0.2 285,150 - -0.6 285,349 - +0.1 285,469 + GFF2223 0.22 +0.2 285,828 + GFF2223 0.72 -1.0 285,829 - GFF2223 0.73 +0.3 285,868 + GFF2223 0.78 -0.2 285,921 + GFF2223 0.85 -0.5 285,922 - GFF2223 0.85 -0.6 285,922 - GFF2223 0.85 -1.5 285,922 - GFF2223 0.85 +0.6 285,922 - GFF2223 0.85 -2.8 285,922 - GFF2223 0.85 -0.1 285,922 - GFF2223 0.85 -0.9 285,922 - GFF2223 0.85 +0.4 285,982 + -0.8 285,996 + +0.9 285,996 + +0.1 285,997 - +0.3 286,060 + -0.1 286,060 + -0.6 286,061 - +0.3 286,061 - -0.8
Or see this region's nucleotide sequence