Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF21 and GFF22 overlap by 4 nucleotides GFF22 and GFF23 are separated by 18 nucleotides GFF23 and GFF24 are separated by 8 nucleotides
GFF21 - no description, at 22,495 to 23,631
GFF21
GFF22 - hypothetical protein, at 23,628 to 26,267
GFF22
GFF23 - no description, at 26,286 to 26,720
GFF23
GFF24 - VgrG protein, at 26,729 to 29,071
GFF24
Position (kb) 23
24
25
26
27 Strain fitness (log2 ratio) -1
0
1
2 at 22.755 kb on - strand, within GFF21 at 22.755 kb on - strand, within GFF21 at 23.681 kb on - strand at 23.841 kb on + strand at 23.841 kb on + strand at 25.446 kb on + strand, within GFF22 at 25.446 kb on + strand, within GFF22 at 25.446 kb on + strand, within GFF22 at 25.447 kb on - strand, within GFF22 at 25.675 kb on + strand, within GFF22 at 26.359 kb on + strand, within GFF23 at 26.638 kb on + strand, within GFF23 at 26.638 kb on + strand, within GFF23 at 26.639 kb on - strand, within GFF23 at 26.757 kb on + strand at 26.757 kb on + strand at 26.758 kb on - strand at 26.778 kb on + strand at 26.850 kb on + strand at 26.850 kb on + strand at 26.851 kb on - strand at 26.899 kb on - strand at 26.899 kb on - strand at 27.003 kb on + strand, within GFF24 at 27.015 kb on + strand, within GFF24 at 27.015 kb on + strand, within GFF24 at 27.036 kb on + strand, within GFF24 at 27.036 kb on + strand, within GFF24 at 27.036 kb on + strand, within GFF24 at 27.057 kb on + strand, within GFF24 at 27.057 kb on + strand, within GFF24 at 27.057 kb on + strand, within GFF24 at 27.058 kb on - strand, within GFF24 at 27.058 kb on - strand, within GFF24 at 27.058 kb on - strand, within GFF24 at 27.058 kb on - strand, within GFF24 at 27.078 kb on + strand, within GFF24 at 27.078 kb on + strand, within GFF24
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 22,755 - GFF21 0.23 -0.1 22,755 - GFF21 0.23 +1.4 23,681 - -0.3 23,841 + -0.2 23,841 + +0.0 25,446 + GFF22 0.69 +0.5 25,446 + GFF22 0.69 -0.2 25,446 + GFF22 0.69 +0.4 25,447 - GFF22 0.69 -0.9 25,675 + GFF22 0.78 +0.8 26,359 + GFF23 0.17 -1.2 26,638 + GFF23 0.81 -0.6 26,638 + GFF23 0.81 +0.6 26,639 - GFF23 0.81 -0.1 26,757 + -1.2 26,757 + -0.2 26,758 - -0.2 26,778 + -0.0 26,850 + +2.1 26,850 + -0.2 26,851 - -1.4 26,899 - -0.9 26,899 - +1.1 27,003 + GFF24 0.12 -0.4 27,015 + GFF24 0.12 -0.7 27,015 + GFF24 0.12 -0.6 27,036 + GFF24 0.13 +0.6 27,036 + GFF24 0.13 +0.3 27,036 + GFF24 0.13 +0.2 27,057 + GFF24 0.14 -0.1 27,057 + GFF24 0.14 +0.7 27,057 + GFF24 0.14 -0.9 27,058 - GFF24 0.14 +0.2 27,058 - GFF24 0.14 -1.2 27,058 - GFF24 0.14 -0.4 27,058 - GFF24 0.14 -0.5 27,078 + GFF24 0.15 -0.1 27,078 + GFF24 0.15 -0.3
Or see this region's nucleotide sequence