Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2167 and GFF2168 are separated by 31 nucleotides GFF2168 and GFF2169 overlap by 4 nucleotides GFF2169 and GFF2170 are separated by 20 nucleotides GFF2170 and GFF2171 are separated by 34 nucleotides
GFF2167 - ABC transporter, substrate-binding protein (cluster 4, leucine/isoleucine/valine/benzoate), at 220,681 to 221,880
GFF2167
GFF2168 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 221,912 to 222,835
GFF2168
GFF2169 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate) / ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 222,832 to 224,622
GFF2169
GFF2170 - Branched-chain amino acid ABC transporter, ATP-binding protein LivF (TC 3.A.1.4.1), at 224,643 to 225,353
GFF2170
GFF2171 - MBL-fold metallohydrolase SPO0683, at 225,388 to 226,356
GFF2171
Position (kb) 222
223
224
225 Strain fitness (log2 ratio) -2
-1
0
1
2 at 221.879 kb on - strand at 221.879 kb on - strand at 221.919 kb on + strand at 221.919 kb on + strand at 221.919 kb on + strand at 221.919 kb on + strand at 221.919 kb on + strand at 221.966 kb on + strand at 221.967 kb on - strand at 222.200 kb on + strand, within GFF2168 at 222.201 kb on - strand, within GFF2168 at 222.201 kb on - strand, within GFF2168 at 222.201 kb on - strand, within GFF2168 at 222.831 kb on + strand at 222.831 kb on + strand at 222.831 kb on + strand at 222.831 kb on + strand at 222.832 kb on - strand at 222.832 kb on - strand at 222.832 kb on - strand at 222.832 kb on - strand at 222.913 kb on + strand at 222.913 kb on + strand at 222.914 kb on - strand at 222.914 kb on - strand at 222.916 kb on + strand at 222.916 kb on + strand at 222.916 kb on + strand at 222.917 kb on - strand at 222.917 kb on - strand at 222.950 kb on - strand at 222.950 kb on - strand at 222.950 kb on - strand at 223.162 kb on + strand, within GFF2169 at 223.162 kb on + strand, within GFF2169 at 223.162 kb on + strand, within GFF2169 at 223.163 kb on - strand, within GFF2169 at 223.163 kb on - strand, within GFF2169 at 223.163 kb on - strand, within GFF2169 at 223.252 kb on + strand, within GFF2169 at 223.252 kb on + strand, within GFF2169 at 223.252 kb on + strand, within GFF2169 at 223.252 kb on + strand, within GFF2169 at 223.252 kb on + strand, within GFF2169 at 223.439 kb on - strand, within GFF2169 at 223.519 kb on + strand, within GFF2169 at 223.520 kb on - strand, within GFF2169 at 223.520 kb on - strand, within GFF2169 at 223.600 kb on + strand, within GFF2169 at 223.601 kb on - strand, within GFF2169 at 223.601 kb on - strand, within GFF2169 at 223.601 kb on - strand, within GFF2169 at 223.601 kb on - strand, within GFF2169 at 223.708 kb on + strand, within GFF2169 at 223.708 kb on + strand, within GFF2169 at 223.709 kb on - strand, within GFF2169 at 223.709 kb on - strand, within GFF2169 at 223.709 kb on - strand, within GFF2169 at 223.861 kb on + strand, within GFF2169 at 224.284 kb on + strand, within GFF2169 at 224.500 kb on + strand at 224.566 kb on + strand at 224.673 kb on + strand at 224.673 kb on + strand at 224.946 kb on + strand, within GFF2170 at 225.259 kb on - strand, within GFF2170 at 225.259 kb on - strand, within GFF2170 at 225.473 kb on - strand at 225.473 kb on - strand at 225.610 kb on + strand, within GFF2171 at 225.610 kb on + strand, within GFF2171 at 225.611 kb on - strand, within GFF2171 at 225.611 kb on - strand, within GFF2171
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 221,879 - -0.4 221,879 - +1.8 221,919 + -0.3 221,919 + +0.3 221,919 + +0.1 221,919 + +0.5 221,919 + -1.0 221,966 + -0.3 221,967 - -0.5 222,200 + GFF2168 0.31 +0.3 222,201 - GFF2168 0.31 -0.5 222,201 - GFF2168 0.31 -0.1 222,201 - GFF2168 0.31 -1.0 222,831 + -0.5 222,831 + -0.5 222,831 + -0.8 222,831 + -1.2 222,832 - -0.8 222,832 - -0.2 222,832 - +0.5 222,832 - -0.2 222,913 + -0.7 222,913 + -1.7 222,914 - -0.2 222,914 - +0.1 222,916 + +0.4 222,916 + -0.6 222,916 + -0.6 222,917 - -0.3 222,917 - -0.4 222,950 - -1.0 222,950 - +0.5 222,950 - +1.0 223,162 + GFF2169 0.18 -1.3 223,162 + GFF2169 0.18 -0.6 223,162 + GFF2169 0.18 -0.7 223,163 - GFF2169 0.18 -0.5 223,163 - GFF2169 0.18 -0.3 223,163 - GFF2169 0.18 -0.6 223,252 + GFF2169 0.23 -0.7 223,252 + GFF2169 0.23 -1.2 223,252 + GFF2169 0.23 -0.8 223,252 + GFF2169 0.23 +0.4 223,252 + GFF2169 0.23 -0.6 223,439 - GFF2169 0.34 -2.1 223,519 + GFF2169 0.38 -0.1 223,520 - GFF2169 0.38 +1.0 223,520 - GFF2169 0.38 +0.4 223,600 + GFF2169 0.43 +0.7 223,601 - GFF2169 0.43 -0.3 223,601 - GFF2169 0.43 -1.4 223,601 - GFF2169 0.43 -0.5 223,601 - GFF2169 0.43 -0.6 223,708 + GFF2169 0.49 -2.0 223,708 + GFF2169 0.49 -0.1 223,709 - GFF2169 0.49 -0.4 223,709 - GFF2169 0.49 -0.8 223,709 - GFF2169 0.49 +0.2 223,861 + GFF2169 0.57 +1.1 224,284 + GFF2169 0.81 -0.1 224,500 + -0.5 224,566 + -0.2 224,673 + +0.2 224,673 + -1.7 224,946 + GFF2170 0.43 -0.1 225,259 - GFF2170 0.87 -0.2 225,259 - GFF2170 0.87 +1.3 225,473 - -2.0 225,473 - -0.7 225,610 + GFF2171 0.23 +0.3 225,610 + GFF2171 0.23 -0.6 225,611 - GFF2171 0.23 -1.4 225,611 - GFF2171 0.23 +0.3
Or see this region's nucleotide sequence