Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2165 and GFF2166 are separated by 63 nucleotides GFF2166 and GFF2167 are separated by 55 nucleotides GFF2167 and GFF2168 are separated by 31 nucleotides GFF2168 and GFF2169 overlap by 4 nucleotides
GFF2165 - Succinate dehydrogenase/fumarate reductase, flavoprotein subunit, at 218,025 to 219,785
GFF2165
GFF2166 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 219,849 to 220,625
GFF2166
GFF2167 - ABC transporter, substrate-binding protein (cluster 4, leucine/isoleucine/valine/benzoate), at 220,681 to 221,880
GFF2167
GFF2168 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 221,912 to 222,835
GFF2168
GFF2169 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate) / ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 222,832 to 224,622
GFF2169
Position (kb) 220
221
222 Strain fitness (log2 ratio) -2
-1
0
1
2 at 219.688 kb on - strand at 219.830 kb on + strand at 219.830 kb on + strand at 219.830 kb on + strand at 219.830 kb on + strand at 219.830 kb on + strand at 219.830 kb on + strand at 219.830 kb on + strand at 219.830 kb on + strand at 219.830 kb on + strand at 219.830 kb on + strand at 219.830 kb on + strand at 219.830 kb on + strand at 219.830 kb on + strand at 219.831 kb on - strand at 219.831 kb on - strand at 219.831 kb on - strand at 219.831 kb on - strand at 219.831 kb on - strand at 219.831 kb on - strand at 219.831 kb on - strand at 219.831 kb on - strand at 219.831 kb on - strand at 219.831 kb on - strand at 219.831 kb on - strand at 219.831 kb on - strand at 219.831 kb on - strand at 220.335 kb on + strand, within GFF2166 at 220.575 kb on + strand at 220.575 kb on + strand at 220.576 kb on - strand at 220.576 kb on - strand at 220.576 kb on - strand at 220.700 kb on - strand at 220.700 kb on - strand at 220.700 kb on - strand at 220.700 kb on - strand at 220.774 kb on + strand at 220.774 kb on + strand at 220.774 kb on + strand at 220.775 kb on - strand at 220.775 kb on - strand at 220.775 kb on - strand at 220.856 kb on + strand, within GFF2167 at 221.110 kb on + strand, within GFF2167 at 221.110 kb on + strand, within GFF2167 at 221.110 kb on + strand, within GFF2167 at 221.110 kb on + strand, within GFF2167 at 221.110 kb on + strand, within GFF2167 at 221.110 kb on + strand, within GFF2167 at 221.111 kb on - strand, within GFF2167 at 221.111 kb on - strand, within GFF2167 at 221.111 kb on - strand, within GFF2167 at 221.164 kb on + strand, within GFF2167 at 221.428 kb on + strand, within GFF2167 at 221.429 kb on - strand, within GFF2167 at 221.429 kb on - strand, within GFF2167 at 221.429 kb on - strand, within GFF2167 at 221.429 kb on - strand, within GFF2167 at 221.476 kb on + strand, within GFF2167 at 221.476 kb on + strand, within GFF2167 at 221.623 kb on + strand, within GFF2167 at 221.624 kb on - strand, within GFF2167 at 221.624 kb on - strand, within GFF2167 at 221.624 kb on - strand, within GFF2167 at 221.740 kb on + strand, within GFF2167 at 221.740 kb on + strand, within GFF2167 at 221.741 kb on - strand, within GFF2167 at 221.830 kb on + strand at 221.831 kb on - strand at 221.879 kb on - strand at 221.879 kb on - strand at 221.919 kb on + strand at 221.919 kb on + strand at 221.919 kb on + strand at 221.919 kb on + strand at 221.919 kb on + strand at 221.966 kb on + strand at 221.967 kb on - strand at 222.200 kb on + strand, within GFF2168 at 222.201 kb on - strand, within GFF2168 at 222.201 kb on - strand, within GFF2168 at 222.201 kb on - strand, within GFF2168 at 222.831 kb on + strand at 222.831 kb on + strand at 222.831 kb on + strand at 222.831 kb on + strand at 222.832 kb on - strand at 222.832 kb on - strand at 222.832 kb on - strand at 222.832 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 219,688 - -0.2 219,830 + -0.4 219,830 + -0.6 219,830 + +0.1 219,830 + +0.3 219,830 + -1.5 219,830 + -0.2 219,830 + -0.5 219,830 + -0.0 219,830 + -1.2 219,830 + -0.3 219,830 + -0.6 219,830 + -0.1 219,830 + +0.1 219,831 - -2.3 219,831 - +0.5 219,831 - -0.2 219,831 - -2.1 219,831 - +0.2 219,831 - +0.4 219,831 - -1.3 219,831 - +0.2 219,831 - -0.6 219,831 - +1.3 219,831 - -1.2 219,831 - -0.0 219,831 - -0.7 220,335 + GFF2166 0.63 +0.1 220,575 + +0.1 220,575 + +1.0 220,576 - +0.1 220,576 - +0.8 220,576 - +0.3 220,700 - -0.4 220,700 - -0.4 220,700 - -2.1 220,700 - -1.5 220,774 + -0.1 220,774 + +0.2 220,774 + -0.6 220,775 - -0.5 220,775 - +0.2 220,775 - +1.3 220,856 + GFF2167 0.15 +0.8 221,110 + GFF2167 0.36 +0.1 221,110 + GFF2167 0.36 -0.6 221,110 + GFF2167 0.36 +1.1 221,110 + GFF2167 0.36 -1.5 221,110 + GFF2167 0.36 -0.4 221,110 + GFF2167 0.36 +0.3 221,111 - GFF2167 0.36 -1.7 221,111 - GFF2167 0.36 -1.0 221,111 - GFF2167 0.36 +0.6 221,164 + GFF2167 0.40 -1.7 221,428 + GFF2167 0.62 -0.5 221,429 - GFF2167 0.62 -0.2 221,429 - GFF2167 0.62 -0.2 221,429 - GFF2167 0.62 -0.3 221,429 - GFF2167 0.62 -0.5 221,476 + GFF2167 0.66 -0.1 221,476 + GFF2167 0.66 -0.5 221,623 + GFF2167 0.79 -0.3 221,624 - GFF2167 0.79 +1.1 221,624 - GFF2167 0.79 -0.9 221,624 - GFF2167 0.79 -1.0 221,740 + GFF2167 0.88 -0.4 221,740 + GFF2167 0.88 -1.3 221,741 - GFF2167 0.88 +0.0 221,830 + -0.3 221,831 - +0.3 221,879 - -0.4 221,879 - +1.8 221,919 + -0.3 221,919 + +0.3 221,919 + +0.1 221,919 + +0.5 221,919 + -1.0 221,966 + -0.3 221,967 - -0.5 222,200 + GFF2168 0.31 +0.3 222,201 - GFF2168 0.31 -0.5 222,201 - GFF2168 0.31 -0.1 222,201 - GFF2168 0.31 -1.0 222,831 + -0.5 222,831 + -0.5 222,831 + -0.8 222,831 + -1.2 222,832 - -0.8 222,832 - -0.2 222,832 - +0.5 222,832 - -0.2
Or see this region's nucleotide sequence