Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2047 and GFF2048 overlap by 4 nucleotides GFF2048 and GFF2049 are separated by 32 nucleotides GFF2049 and GFF2050 are separated by 26 nucleotides GFF2050 and GFF2051 are separated by 156 nucleotides
GFF2047 - Polyhydroxyalkanoic acid synthase, at 95,473 to 96,546
GFF2047
GFF2048 - hypothetical protein, at 96,543 to 96,950
GFF2048
GFF2049 - Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24), at 96,983 to 97,903
GFF2049
GFF2050 - hypothetical protein, at 97,930 to 98,430
GFF2050
GFF2051 - FIG00463476: hypothetical protein, at 98,587 to 99,234
GFF2051
Position (kb)
96
97
98 Strain fitness (log2 ratio)
-1
0
1
2
3 at 96.078 kb on - strand, within GFF2047 at 96.185 kb on + strand, within GFF2047 at 96.186 kb on - strand, within GFF2047 at 96.186 kb on - strand, within GFF2047 at 96.186 kb on - strand, within GFF2047 at 96.242 kb on + strand, within GFF2047 at 96.242 kb on + strand, within GFF2047 at 96.242 kb on + strand, within GFF2047 at 96.242 kb on + strand, within GFF2047 at 96.242 kb on + strand, within GFF2047 at 96.242 kb on + strand, within GFF2047 at 96.243 kb on - strand, within GFF2047 at 96.251 kb on + strand, within GFF2047 at 96.251 kb on + strand, within GFF2047 at 96.252 kb on - strand, within GFF2047 at 96.269 kb on + strand, within GFF2047 at 96.269 kb on + strand, within GFF2047 at 96.269 kb on + strand, within GFF2047 at 96.270 kb on - strand, within GFF2047 at 96.308 kb on + strand, within GFF2047 at 96.308 kb on + strand, within GFF2047 at 96.308 kb on + strand, within GFF2047 at 96.309 kb on - strand, within GFF2047 at 96.309 kb on - strand, within GFF2047 at 96.354 kb on - strand, within GFF2047 at 96.354 kb on - strand, within GFF2047 at 96.377 kb on + strand, within GFF2047 at 96.378 kb on - strand, within GFF2047 at 96.383 kb on + strand, within GFF2047 at 96.383 kb on + strand, within GFF2047 at 96.384 kb on - strand, within GFF2047 at 96.384 kb on - strand, within GFF2047 at 96.384 kb on - strand, within GFF2047 at 96.384 kb on - strand, within GFF2047 at 97.362 kb on + strand, within GFF2049 at 97.363 kb on - strand, within GFF2049 at 97.363 kb on - strand, within GFF2049 at 97.363 kb on - strand, within GFF2049 at 97.363 kb on - strand, within GFF2049 at 97.605 kb on + strand, within GFF2049 at 97.606 kb on - strand, within GFF2049 at 97.606 kb on - strand, within GFF2049 at 97.606 kb on - strand, within GFF2049 at 97.606 kb on - strand, within GFF2049 at 97.931 kb on + strand at 97.931 kb on + strand at 97.931 kb on + strand at 97.931 kb on + strand at 97.931 kb on + strand at 97.931 kb on + strand at 97.932 kb on - strand at 98.207 kb on + strand, within GFF2050 at 98.207 kb on + strand, within GFF2050 at 98.208 kb on - strand, within GFF2050 at 98.505 kb on + strand at 98.506 kb on - strand at 98.578 kb on - strand at 98.578 kb on - strand at 98.588 kb on + strand at 98.588 kb on + strand at 98.588 kb on + strand at 98.588 kb on + strand at 98.588 kb on + strand at 98.588 kb on + strand at 98.588 kb on + strand at 98.588 kb on + strand at 98.589 kb on - strand at 98.589 kb on - strand at 98.589 kb on - strand at 98.589 kb on - strand at 98.589 kb on - strand at 98.589 kb on - strand at 98.589 kb on - strand at 98.589 kb on - strand at 98.768 kb on + strand, within GFF2051 at 98.768 kb on + strand, within GFF2051 at 98.768 kb on + strand, within GFF2051 at 98.768 kb on + strand, within GFF2051 at 98.768 kb on + strand, within GFF2051 at 98.768 kb on + strand, within GFF2051 at 98.769 kb on - strand, within GFF2051 at 98.769 kb on - strand, within GFF2051 at 98.769 kb on - strand, within GFF2051 at 98.769 kb on - strand, within GFF2051 at 98.769 kb on - strand, within GFF2051 at 98.769 kb on - strand, within GFF2051 at 98.769 kb on - strand, within GFF2051
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 96,078 - GFF2047 0.56 -0.5 96,185 + GFF2047 0.66 -0.6 96,186 - GFF2047 0.66 -0.6 96,186 - GFF2047 0.66 +0.0 96,186 - GFF2047 0.66 -0.6 96,242 + GFF2047 0.72 +0.3 96,242 + GFF2047 0.72 +0.6 96,242 + GFF2047 0.72 -0.4 96,242 + GFF2047 0.72 +1.0 96,242 + GFF2047 0.72 -0.1 96,242 + GFF2047 0.72 -1.4 96,243 - GFF2047 0.72 -1.0 96,251 + GFF2047 0.72 -0.1 96,251 + GFF2047 0.72 +0.1 96,252 - GFF2047 0.73 +0.2 96,269 + GFF2047 0.74 -0.6 96,269 + GFF2047 0.74 -0.2 96,269 + GFF2047 0.74 -1.7 96,270 - GFF2047 0.74 -0.7 96,308 + GFF2047 0.78 +1.7 96,308 + GFF2047 0.78 +2.5 96,308 + GFF2047 0.78 -1.6 96,309 - GFF2047 0.78 +1.0 96,309 - GFF2047 0.78 +0.0 96,354 - GFF2047 0.82 +1.9 96,354 - GFF2047 0.82 +0.8 96,377 + GFF2047 0.84 +0.7 96,378 - GFF2047 0.84 -0.4 96,383 + GFF2047 0.85 -1.4 96,383 + GFF2047 0.85 -0.4 96,384 - GFF2047 0.85 +0.1 96,384 - GFF2047 0.85 +1.9 96,384 - GFF2047 0.85 +0.3 96,384 - GFF2047 0.85 -1.2 97,362 + GFF2049 0.41 +0.6 97,363 - GFF2049 0.41 +0.7 97,363 - GFF2049 0.41 -0.1 97,363 - GFF2049 0.41 +1.0 97,363 - GFF2049 0.41 +0.6 97,605 + GFF2049 0.68 +1.0 97,606 - GFF2049 0.68 -0.4 97,606 - GFF2049 0.68 +0.3 97,606 - GFF2049 0.68 -0.2 97,606 - GFF2049 0.68 +0.0 97,931 + +1.9 97,931 + -0.1 97,931 + -0.7 97,931 + -0.4 97,931 + -0.4 97,931 + -0.3 97,932 - -0.9 98,207 + GFF2050 0.55 -0.0 98,207 + GFF2050 0.55 -0.1 98,208 - GFF2050 0.55 -0.3 98,505 + -0.7 98,506 - -0.4 98,578 - +1.0 98,578 - +0.6 98,588 + -1.1 98,588 + -0.6 98,588 + -1.3 98,588 + +0.9 98,588 + -0.1 98,588 + -0.5 98,588 + +0.2 98,588 + -0.3 98,589 - -1.3 98,589 - -0.6 98,589 - -0.2 98,589 - -0.0 98,589 - +3.5 98,589 - -0.1 98,589 - -0.1 98,589 - -0.3 98,768 + GFF2051 0.28 -0.1 98,768 + GFF2051 0.28 -0.6 98,768 + GFF2051 0.28 +0.7 98,768 + GFF2051 0.28 -1.1 98,768 + GFF2051 0.28 -0.7 98,768 + GFF2051 0.28 +0.3 98,769 - GFF2051 0.28 +0.1 98,769 - GFF2051 0.28 -0.4 98,769 - GFF2051 0.28 -0.1 98,769 - GFF2051 0.28 +1.9 98,769 - GFF2051 0.28 -0.8 98,769 - GFF2051 0.28 -1.7 98,769 - GFF2051 0.28 +0.2
Or see this region's nucleotide sequence