Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2021 and GFF2022 overlap by 4 nucleotides GFF2022 and GFF2023 are separated by 137 nucleotides
GFF2021 - FIG00538369: hypothetical protein, at 64,703 to 65,926
GFF2021
GFF2022 - Acyl-CoA dehydrogenase, at 65,923 to 67,092
GFF2022
GFF2023 - Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4), at 67,230 to 68,150
GFF2023
Position (kb)
65
66
67
68 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 64.988 kb on + strand, within GFF2021 at 64.989 kb on - strand, within GFF2021 at 64.989 kb on - strand, within GFF2021 at 65.031 kb on - strand, within GFF2021 at 65.066 kb on + strand, within GFF2021 at 65.066 kb on + strand, within GFF2021 at 65.067 kb on - strand, within GFF2021 at 65.085 kb on - strand, within GFF2021 at 65.102 kb on + strand, within GFF2021 at 65.102 kb on + strand, within GFF2021 at 65.420 kb on + strand, within GFF2021 at 65.421 kb on - strand, within GFF2021 at 65.456 kb on + strand, within GFF2021 at 65.457 kb on - strand, within GFF2021 at 65.567 kb on + strand, within GFF2021 at 65.567 kb on + strand, within GFF2021 at 65.567 kb on + strand, within GFF2021 at 65.567 kb on + strand, within GFF2021 at 65.568 kb on - strand, within GFF2021 at 65.568 kb on - strand, within GFF2021 at 65.568 kb on - strand, within GFF2021 at 65.568 kb on - strand, within GFF2021 at 65.708 kb on + strand, within GFF2021 at 66.001 kb on + strand at 66.002 kb on - strand at 66.493 kb on + strand, within GFF2022 at 66.493 kb on + strand, within GFF2022 at 66.494 kb on - strand, within GFF2022 at 66.494 kb on - strand, within GFF2022 at 66.494 kb on - strand, within GFF2022 at 66.637 kb on + strand, within GFF2022 at 66.637 kb on + strand, within GFF2022 at 66.637 kb on + strand, within GFF2022 at 66.638 kb on - strand, within GFF2022 at 66.638 kb on - strand, within GFF2022 at 66.638 kb on - strand, within GFF2022 at 66.638 kb on - strand, within GFF2022 at 66.727 kb on + strand, within GFF2022 at 66.728 kb on - strand, within GFF2022 at 66.728 kb on - strand, within GFF2022 at 66.728 kb on - strand, within GFF2022 at 66.853 kb on + strand, within GFF2022 at 66.898 kb on + strand, within GFF2022 at 66.898 kb on + strand, within GFF2022 at 66.898 kb on + strand, within GFF2022 at 66.898 kb on + strand, within GFF2022 at 66.898 kb on + strand, within GFF2022 at 66.899 kb on - strand, within GFF2022 at 66.899 kb on - strand, within GFF2022 at 66.964 kb on + strand, within GFF2022 at 66.964 kb on + strand, within GFF2022 at 66.965 kb on - strand, within GFF2022 at 66.976 kb on + strand at 66.976 kb on + strand at 66.977 kb on - strand at 66.977 kb on - strand at 66.977 kb on - strand at 66.977 kb on - strand at 66.977 kb on - strand at 66.977 kb on - strand at 66.977 kb on - strand at 66.977 kb on - strand at 67.006 kb on + strand at 67.006 kb on + strand at 67.006 kb on + strand at 67.006 kb on + strand at 67.006 kb on + strand at 67.006 kb on + strand at 67.006 kb on + strand at 67.006 kb on + strand at 67.006 kb on + strand at 67.006 kb on + strand at 67.006 kb on + strand at 67.007 kb on - strand at 67.007 kb on - strand at 67.007 kb on - strand at 67.007 kb on - strand at 67.007 kb on - strand at 67.010 kb on + strand at 67.010 kb on + strand at 67.010 kb on + strand at 67.087 kb on + strand at 67.087 kb on + strand at 67.088 kb on - strand at 67.137 kb on + strand at 67.137 kb on + strand at 67.138 kb on - strand at 67.138 kb on - strand at 67.138 kb on - strand at 67.155 kb on + strand at 67.155 kb on + strand at 67.156 kb on - strand at 67.156 kb on - strand at 67.458 kb on + strand, within GFF2023 at 67.458 kb on + strand, within GFF2023 at 67.459 kb on - strand, within GFF2023 at 67.723 kb on - strand, within GFF2023 at 67.875 kb on + strand, within GFF2023 at 67.875 kb on + strand, within GFF2023 at 67.876 kb on - strand, within GFF2023 at 67.966 kb on - strand, within GFF2023 at 68.010 kb on + strand, within GFF2023 at 68.010 kb on + strand, within GFF2023 at 68.010 kb on + strand, within GFF2023 at 68.010 kb on + strand, within GFF2023 at 68.011 kb on - strand, within GFF2023 at 68.011 kb on - strand, within GFF2023 at 68.011 kb on - strand, within GFF2023 at 68.011 kb on - strand, within GFF2023 at 68.011 kb on - strand, within GFF2023 at 68.011 kb on - strand, within GFF2023 at 68.076 kb on + strand at 68.076 kb on + strand at 68.076 kb on + strand at 68.076 kb on + strand at 68.076 kb on + strand at 68.076 kb on + strand at 68.077 kb on - strand at 68.077 kb on - strand at 68.077 kb on - strand at 68.077 kb on - strand at 68.077 kb on - strand at 68.077 kb on - strand at 68.077 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 64,988 + GFF2021 0.23 -0.2 64,989 - GFF2021 0.23 -2.0 64,989 - GFF2021 0.23 -0.1 65,031 - GFF2021 0.27 -0.8 65,066 + GFF2021 0.30 -0.5 65,066 + GFF2021 0.30 -0.0 65,067 - GFF2021 0.30 -1.0 65,085 - GFF2021 0.31 +0.1 65,102 + GFF2021 0.33 -2.8 65,102 + GFF2021 0.33 -0.5 65,420 + GFF2021 0.59 -0.5 65,421 - GFF2021 0.59 -0.8 65,456 + GFF2021 0.62 +0.3 65,457 - GFF2021 0.62 -0.9 65,567 + GFF2021 0.71 -0.4 65,567 + GFF2021 0.71 +0.4 65,567 + GFF2021 0.71 +0.5 65,567 + GFF2021 0.71 -0.9 65,568 - GFF2021 0.71 -0.4 65,568 - GFF2021 0.71 -0.4 65,568 - GFF2021 0.71 -0.8 65,568 - GFF2021 0.71 -0.8 65,708 + GFF2021 0.82 -0.2 66,001 + -0.7 66,002 - -0.4 66,493 + GFF2022 0.49 -1.4 66,493 + GFF2022 0.49 -1.4 66,494 - GFF2022 0.49 -1.3 66,494 - GFF2022 0.49 -0.5 66,494 - GFF2022 0.49 +0.2 66,637 + GFF2022 0.61 -0.0 66,637 + GFF2022 0.61 -0.0 66,637 + GFF2022 0.61 +0.5 66,638 - GFF2022 0.61 -0.3 66,638 - GFF2022 0.61 -0.6 66,638 - GFF2022 0.61 -0.2 66,638 - GFF2022 0.61 -1.1 66,727 + GFF2022 0.69 +0.7 66,728 - GFF2022 0.69 +0.9 66,728 - GFF2022 0.69 +0.3 66,728 - GFF2022 0.69 +0.1 66,853 + GFF2022 0.79 -0.7 66,898 + GFF2022 0.83 -0.3 66,898 + GFF2022 0.83 +0.3 66,898 + GFF2022 0.83 +1.3 66,898 + GFF2022 0.83 -0.3 66,898 + GFF2022 0.83 -0.3 66,899 - GFF2022 0.83 +0.9 66,899 - GFF2022 0.83 -0.3 66,964 + GFF2022 0.89 +0.3 66,964 + GFF2022 0.89 -0.3 66,965 - GFF2022 0.89 +1.1 66,976 + -0.2 66,976 + -0.8 66,977 - -0.2 66,977 - +0.3 66,977 - -0.4 66,977 - +0.1 66,977 - +0.4 66,977 - -0.1 66,977 - +0.4 66,977 - +0.1 67,006 + -1.3 67,006 + +0.3 67,006 + -1.4 67,006 + +0.7 67,006 + +0.2 67,006 + -1.6 67,006 + -0.0 67,006 + +0.0 67,006 + +0.5 67,006 + +0.1 67,006 + +0.9 67,007 - +0.1 67,007 - +0.7 67,007 - +1.9 67,007 - +0.5 67,007 - -0.2 67,010 + -0.4 67,010 + +0.1 67,010 + -1.9 67,087 + -0.4 67,087 + +0.2 67,088 - -0.1 67,137 + -0.2 67,137 + +0.1 67,138 - +2.2 67,138 - -1.0 67,138 - +0.3 67,155 + +0.1 67,155 + -0.1 67,156 - -0.1 67,156 - -0.9 67,458 + GFF2023 0.25 +0.9 67,458 + GFF2023 0.25 -0.8 67,459 - GFF2023 0.25 -0.4 67,723 - GFF2023 0.54 -0.7 67,875 + GFF2023 0.70 -1.1 67,875 + GFF2023 0.70 -0.1 67,876 - GFF2023 0.70 -0.8 67,966 - GFF2023 0.80 -0.5 68,010 + GFF2023 0.85 -0.2 68,010 + GFF2023 0.85 -0.9 68,010 + GFF2023 0.85 -0.5 68,010 + GFF2023 0.85 +0.1 68,011 - GFF2023 0.85 +0.1 68,011 - GFF2023 0.85 +0.3 68,011 - GFF2023 0.85 -0.2 68,011 - GFF2023 0.85 +0.7 68,011 - GFF2023 0.85 +0.0 68,011 - GFF2023 0.85 -2.0 68,076 + +0.1 68,076 + -1.2 68,076 + -0.9 68,076 + -0.7 68,076 + -0.2 68,076 + +0.7 68,077 - +1.1 68,077 - +0.8 68,077 - -0.0 68,077 - -0.3 68,077 - -0.8 68,077 - -2.4 68,077 - -0.2
Or see this region's nucleotide sequence