Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2006 and GFF2007 are separated by 143 nucleotides GFF2007 and GFF2008 overlap by 4 nucleotides
GFF2006 - Isovaleryl-CoA dehydrogenase (EC 1.3.8.4), at 49,632 to 50,804
GFF2006
GFF2007 - Acetoacetyl-CoA synthetase [leucine] (EC 6.2.1.16), at 50,948 to 52,627
GFF2007
GFF2008 - Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4), at 52,624 to 54,252
GFF2008
Position (kb)
50
51
52
53 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 49.974 kb on + strand, within GFF2006 at 49.974 kb on + strand, within GFF2006 at 49.974 kb on + strand, within GFF2006 at 49.974 kb on + strand, within GFF2006 at 49.974 kb on + strand, within GFF2006 at 49.974 kb on + strand, within GFF2006 at 49.975 kb on - strand, within GFF2006 at 49.975 kb on - strand, within GFF2006 at 49.975 kb on - strand, within GFF2006 at 49.975 kb on - strand, within GFF2006 at 49.975 kb on - strand, within GFF2006 at 49.975 kb on - strand, within GFF2006 at 50.085 kb on + strand, within GFF2006 at 50.085 kb on + strand, within GFF2006 at 50.086 kb on - strand, within GFF2006 at 50.088 kb on + strand, within GFF2006 at 50.088 kb on + strand, within GFF2006 at 50.088 kb on + strand, within GFF2006 at 50.088 kb on + strand, within GFF2006 at 50.088 kb on + strand, within GFF2006 at 50.088 kb on + strand, within GFF2006 at 50.088 kb on + strand, within GFF2006 at 50.088 kb on + strand, within GFF2006 at 50.088 kb on + strand, within GFF2006 at 50.088 kb on + strand, within GFF2006 at 50.089 kb on - strand, within GFF2006 at 50.089 kb on - strand, within GFF2006 at 50.089 kb on - strand, within GFF2006 at 50.089 kb on - strand, within GFF2006 at 50.089 kb on - strand, within GFF2006 at 50.089 kb on - strand, within GFF2006 at 50.089 kb on - strand, within GFF2006 at 50.089 kb on - strand, within GFF2006 at 50.089 kb on - strand, within GFF2006 at 50.089 kb on - strand, within GFF2006 at 50.089 kb on - strand, within GFF2006 at 50.089 kb on - strand, within GFF2006 at 50.151 kb on + strand, within GFF2006 at 50.151 kb on + strand, within GFF2006 at 50.151 kb on + strand, within GFF2006 at 50.152 kb on - strand, within GFF2006 at 50.152 kb on - strand, within GFF2006 at 50.152 kb on - strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.511 kb on + strand, within GFF2006 at 50.512 kb on - strand, within GFF2006 at 50.512 kb on - strand, within GFF2006 at 50.512 kb on - strand, within GFF2006 at 50.512 kb on - strand, within GFF2006 at 50.512 kb on - strand, within GFF2006 at 50.512 kb on - strand, within GFF2006 at 50.512 kb on - strand, within GFF2006 at 50.538 kb on + strand, within GFF2006 at 50.538 kb on + strand, within GFF2006 at 50.538 kb on + strand, within GFF2006 at 50.544 kb on + strand, within GFF2006 at 50.682 kb on + strand, within GFF2006 at 50.730 kb on + strand at 50.731 kb on - strand at 50.731 kb on - strand at 50.731 kb on - strand at 50.738 kb on - strand at 50.802 kb on + strand at 50.802 kb on + strand at 50.802 kb on + strand at 50.802 kb on + strand at 50.802 kb on + strand at 50.802 kb on + strand at 50.802 kb on + strand at 50.802 kb on + strand at 50.803 kb on - strand at 50.803 kb on - strand at 50.803 kb on - strand at 50.803 kb on - strand at 50.979 kb on - strand at 50.979 kb on - strand at 50.979 kb on - strand at 51.105 kb on - strand at 51.105 kb on - strand at 51.272 kb on + strand, within GFF2007 at 51.273 kb on - strand, within GFF2007 at 51.294 kb on - strand, within GFF2007 at 51.294 kb on - strand, within GFF2007 at 51.294 kb on - strand, within GFF2007 at 51.356 kb on + strand, within GFF2007 at 51.357 kb on - strand, within GFF2007 at 51.357 kb on - strand, within GFF2007 at 51.689 kb on + strand, within GFF2007 at 51.689 kb on + strand, within GFF2007 at 51.689 kb on + strand, within GFF2007 at 51.690 kb on - strand, within GFF2007 at 51.753 kb on - strand, within GFF2007 at 51.983 kb on + strand, within GFF2007 at 52.157 kb on + strand, within GFF2007 at 52.158 kb on - strand, within GFF2007 at 52.265 kb on + strand, within GFF2007 at 52.266 kb on - strand, within GFF2007 at 52.266 kb on - strand, within GFF2007 at 52.266 kb on - strand, within GFF2007 at 52.266 kb on - strand, within GFF2007 at 52.266 kb on - strand, within GFF2007 at 52.266 kb on - strand, within GFF2007 at 52.322 kb on + strand, within GFF2007 at 52.323 kb on - strand, within GFF2007 at 52.323 kb on - strand, within GFF2007 at 52.323 kb on - strand, within GFF2007 at 52.323 kb on - strand, within GFF2007 at 52.323 kb on - strand, within GFF2007 at 52.349 kb on + strand, within GFF2007 at 52.350 kb on - strand, within GFF2007 at 52.404 kb on - strand, within GFF2007 at 52.499 kb on + strand at 52.499 kb on + strand at 52.499 kb on + strand at 52.499 kb on + strand at 52.499 kb on + strand at 52.499 kb on + strand at 52.499 kb on + strand at 52.499 kb on + strand at 52.499 kb on + strand at 52.500 kb on - strand at 52.500 kb on - strand at 52.500 kb on - strand at 52.500 kb on - strand at 52.500 kb on - strand at 52.500 kb on - strand at 52.500 kb on - strand at 52.500 kb on - strand at 52.500 kb on - strand at 52.514 kb on + strand at 52.515 kb on - strand at 52.515 kb on - strand at 52.515 kb on - strand at 52.515 kb on - strand at 52.867 kb on + strand, within GFF2008 at 52.867 kb on + strand, within GFF2008 at 52.867 kb on + strand, within GFF2008 at 52.867 kb on + strand, within GFF2008 at 52.868 kb on - strand, within GFF2008 at 52.868 kb on - strand, within GFF2008 at 52.987 kb on + strand, within GFF2008 at 52.987 kb on + strand, within GFF2008 at 52.987 kb on + strand, within GFF2008 at 52.987 kb on + strand, within GFF2008 at 52.987 kb on + strand, within GFF2008 at 52.987 kb on + strand, within GFF2008 at 52.987 kb on + strand, within GFF2008 at 52.987 kb on + strand, within GFF2008 at 52.987 kb on + strand, within GFF2008 at 52.988 kb on - strand, within GFF2008 at 52.988 kb on - strand, within GFF2008 at 52.988 kb on - strand, within GFF2008 at 52.988 kb on - strand, within GFF2008 at 52.988 kb on - strand, within GFF2008 at 52.988 kb on - strand, within GFF2008 at 52.988 kb on - strand, within GFF2008 at 52.991 kb on - strand, within GFF2008 at 53.059 kb on + strand, within GFF2008 at 53.059 kb on + strand, within GFF2008 at 53.059 kb on + strand, within GFF2008 at 53.059 kb on + strand, within GFF2008 at 53.060 kb on - strand, within GFF2008 at 53.060 kb on - strand, within GFF2008 at 53.060 kb on - strand, within GFF2008 at 53.140 kb on + strand, within GFF2008 at 53.140 kb on + strand, within GFF2008 at 53.140 kb on + strand, within GFF2008 at 53.141 kb on - strand, within GFF2008 at 53.141 kb on - strand, within GFF2008 at 53.141 kb on - strand, within GFF2008 at 53.141 kb on - strand, within GFF2008 at 53.141 kb on - strand, within GFF2008 at 53.218 kb on + strand, within GFF2008 at 53.218 kb on + strand, within GFF2008 at 53.219 kb on - strand, within GFF2008 at 53.512 kb on + strand, within GFF2008 at 53.512 kb on + strand, within GFF2008 at 53.512 kb on + strand, within GFF2008 at 53.512 kb on + strand, within GFF2008 at 53.512 kb on + strand, within GFF2008 at 53.512 kb on + strand, within GFF2008
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 49,974 + GFF2006 0.29 +1.3 49,974 + GFF2006 0.29 -0.4 49,974 + GFF2006 0.29 -0.1 49,974 + GFF2006 0.29 -1.1 49,974 + GFF2006 0.29 -1.4 49,974 + GFF2006 0.29 -1.1 49,975 - GFF2006 0.29 +0.9 49,975 - GFF2006 0.29 +1.9 49,975 - GFF2006 0.29 -0.5 49,975 - GFF2006 0.29 -0.7 49,975 - GFF2006 0.29 -0.3 49,975 - GFF2006 0.29 -0.9 50,085 + GFF2006 0.39 -0.1 50,085 + GFF2006 0.39 +2.6 50,086 - GFF2006 0.39 -0.8 50,088 + GFF2006 0.39 -0.1 50,088 + GFF2006 0.39 -1.6 50,088 + GFF2006 0.39 -0.2 50,088 + GFF2006 0.39 -2.0 50,088 + GFF2006 0.39 +0.6 50,088 + GFF2006 0.39 +0.8 50,088 + GFF2006 0.39 -1.0 50,088 + GFF2006 0.39 +1.1 50,088 + GFF2006 0.39 -0.6 50,088 + GFF2006 0.39 -1.7 50,089 - GFF2006 0.39 +1.0 50,089 - GFF2006 0.39 -1.3 50,089 - GFF2006 0.39 +0.1 50,089 - GFF2006 0.39 -0.5 50,089 - GFF2006 0.39 +0.3 50,089 - GFF2006 0.39 -0.3 50,089 - GFF2006 0.39 -0.8 50,089 - GFF2006 0.39 +0.0 50,089 - GFF2006 0.39 +0.1 50,089 - GFF2006 0.39 -2.4 50,089 - GFF2006 0.39 -0.1 50,089 - GFF2006 0.39 -0.6 50,151 + GFF2006 0.44 -0.1 50,151 + GFF2006 0.44 +0.3 50,151 + GFF2006 0.44 -0.4 50,152 - GFF2006 0.44 +0.1 50,152 - GFF2006 0.44 +0.5 50,152 - GFF2006 0.44 -0.9 50,511 + GFF2006 0.75 +0.4 50,511 + GFF2006 0.75 +0.3 50,511 + GFF2006 0.75 -0.7 50,511 + GFF2006 0.75 -0.3 50,511 + GFF2006 0.75 -0.2 50,511 + GFF2006 0.75 +0.9 50,511 + GFF2006 0.75 -0.7 50,511 + GFF2006 0.75 -1.8 50,511 + GFF2006 0.75 +0.9 50,511 + GFF2006 0.75 -0.2 50,511 + GFF2006 0.75 +0.4 50,511 + GFF2006 0.75 -2.3 50,511 + GFF2006 0.75 -2.8 50,511 + GFF2006 0.75 +0.0 50,511 + GFF2006 0.75 -0.5 50,511 + GFF2006 0.75 -0.2 50,511 + GFF2006 0.75 -0.4 50,511 + GFF2006 0.75 -0.5 50,512 - GFF2006 0.75 +0.5 50,512 - GFF2006 0.75 -2.5 50,512 - GFF2006 0.75 -1.4 50,512 - GFF2006 0.75 +0.0 50,512 - GFF2006 0.75 -0.3 50,512 - GFF2006 0.75 -0.7 50,512 - GFF2006 0.75 +0.4 50,538 + GFF2006 0.77 -0.6 50,538 + GFF2006 0.77 -0.6 50,538 + GFF2006 0.77 -1.1 50,544 + GFF2006 0.78 -0.1 50,682 + GFF2006 0.90 -2.0 50,730 + +0.8 50,731 - -1.4 50,731 - -0.5 50,731 - -0.2 50,738 - +0.6 50,802 + -0.3 50,802 + -0.1 50,802 + -0.0 50,802 + -1.4 50,802 + -0.3 50,802 + -0.4 50,802 + -0.1 50,802 + -0.7 50,803 - -0.2 50,803 - -0.1 50,803 - -0.4 50,803 - +0.6 50,979 - +0.1 50,979 - -0.8 50,979 - -0.4 51,105 - -0.6 51,105 - +0.0 51,272 + GFF2007 0.19 -0.7 51,273 - GFF2007 0.19 -0.4 51,294 - GFF2007 0.21 +0.3 51,294 - GFF2007 0.21 -0.5 51,294 - GFF2007 0.21 -0.3 51,356 + GFF2007 0.24 +0.1 51,357 - GFF2007 0.24 -0.1 51,357 - GFF2007 0.24 -0.4 51,689 + GFF2007 0.44 -0.7 51,689 + GFF2007 0.44 +0.9 51,689 + GFF2007 0.44 -1.5 51,690 - GFF2007 0.44 -0.2 51,753 - GFF2007 0.48 +0.7 51,983 + GFF2007 0.62 +1.9 52,157 + GFF2007 0.72 -0.9 52,158 - GFF2007 0.72 -0.9 52,265 + GFF2007 0.78 -0.6 52,266 - GFF2007 0.78 -0.2 52,266 - GFF2007 0.78 -0.0 52,266 - GFF2007 0.78 +0.1 52,266 - GFF2007 0.78 +0.0 52,266 - GFF2007 0.78 -0.6 52,266 - GFF2007 0.78 -0.2 52,322 + GFF2007 0.82 +0.8 52,323 - GFF2007 0.82 -0.7 52,323 - GFF2007 0.82 +0.0 52,323 - GFF2007 0.82 -1.7 52,323 - GFF2007 0.82 +2.0 52,323 - GFF2007 0.82 -0.2 52,349 + GFF2007 0.83 +0.9 52,350 - GFF2007 0.83 -0.9 52,404 - GFF2007 0.87 -1.1 52,499 + -0.3 52,499 + -0.4 52,499 + -0.1 52,499 + -1.1 52,499 + -1.3 52,499 + -0.3 52,499 + +0.3 52,499 + +0.2 52,499 + -1.1 52,500 - +0.1 52,500 - -0.6 52,500 - -0.3 52,500 - +0.1 52,500 - -0.1 52,500 - -0.0 52,500 - -0.5 52,500 - +0.1 52,500 - -0.5 52,514 + -1.1 52,515 - -0.9 52,515 - -0.6 52,515 - +1.1 52,515 - -0.0 52,867 + GFF2008 0.15 -0.9 52,867 + GFF2008 0.15 -0.3 52,867 + GFF2008 0.15 +0.4 52,867 + GFF2008 0.15 +1.1 52,868 - GFF2008 0.15 +1.9 52,868 - GFF2008 0.15 -0.8 52,987 + GFF2008 0.22 +0.1 52,987 + GFF2008 0.22 -0.4 52,987 + GFF2008 0.22 +1.1 52,987 + GFF2008 0.22 -0.0 52,987 + GFF2008 0.22 -0.1 52,987 + GFF2008 0.22 -0.2 52,987 + GFF2008 0.22 -0.1 52,987 + GFF2008 0.22 -0.3 52,987 + GFF2008 0.22 +0.3 52,988 - GFF2008 0.22 +1.2 52,988 - GFF2008 0.22 -1.6 52,988 - GFF2008 0.22 -1.5 52,988 - GFF2008 0.22 -0.0 52,988 - GFF2008 0.22 +0.9 52,988 - GFF2008 0.22 -2.1 52,988 - GFF2008 0.22 +0.2 52,991 - GFF2008 0.23 -1.2 53,059 + GFF2008 0.27 +0.3 53,059 + GFF2008 0.27 -1.4 53,059 + GFF2008 0.27 +0.4 53,059 + GFF2008 0.27 -0.9 53,060 - GFF2008 0.27 -0.9 53,060 - GFF2008 0.27 +0.8 53,060 - GFF2008 0.27 -1.4 53,140 + GFF2008 0.32 -0.7 53,140 + GFF2008 0.32 -0.4 53,140 + GFF2008 0.32 +0.2 53,141 - GFF2008 0.32 -1.8 53,141 - GFF2008 0.32 +0.5 53,141 - GFF2008 0.32 -1.8 53,141 - GFF2008 0.32 +1.6 53,141 - GFF2008 0.32 -0.4 53,218 + GFF2008 0.36 +0.9 53,218 + GFF2008 0.36 -0.3 53,219 - GFF2008 0.37 +0.5 53,512 + GFF2008 0.55 -1.6 53,512 + GFF2008 0.55 -1.4 53,512 + GFF2008 0.55 -1.1 53,512 + GFF2008 0.55 -0.6 53,512 + GFF2008 0.55 -0.3 53,512 + GFF2008 0.55 -0.2
Or see this region's nucleotide sequence