Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1967 and GFF1968 are separated by 8 nucleotides GFF1968 and GFF1969 overlap by 4 nucleotides GFF1969 and GFF1970 are separated by 64 nucleotides
GFF1967 - FAD linked oxidase-like, at 9,804 to 11,180
GFF1967
GFF1968 - Hydroxymethylpyrimidine ABC transporter, transmembrane component, at 11,189 to 11,995
GFF1968
GFF1969 - ABC transporter, ATP-binding protein (cluster 1, maltose/g3p/polyamine/iron); ABC transporter, ATP-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 11,992 to 12,828
GFF1969
GFF1970 - Hydroxymethylpyrimidine ABC transporter, substrate-binding component, at 12,893 to 13,876
GFF1970
Position (kb)
11
12 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 10.690 kb on + strand, within GFF1967 at 10.691 kb on - strand, within GFF1967 at 10.691 kb on - strand, within GFF1967 at 10.753 kb on + strand, within GFF1967 at 10.873 kb on + strand, within GFF1967 at 10.873 kb on + strand, within GFF1967 at 10.873 kb on + strand, within GFF1967 at 10.873 kb on + strand, within GFF1967 at 10.874 kb on - strand, within GFF1967 at 10.874 kb on - strand, within GFF1967 at 10.924 kb on + strand, within GFF1967 at 10.924 kb on + strand, within GFF1967 at 10.924 kb on + strand, within GFF1967 at 10.924 kb on + strand, within GFF1967 at 10.925 kb on - strand, within GFF1967 at 10.925 kb on - strand, within GFF1967 at 10.925 kb on - strand, within GFF1967 at 11.346 kb on + strand, within GFF1968 at 11.448 kb on + strand, within GFF1968 at 11.448 kb on + strand, within GFF1968 at 11.448 kb on + strand, within GFF1968 at 11.448 kb on + strand, within GFF1968 at 11.448 kb on + strand, within GFF1968 at 11.449 kb on - strand, within GFF1968 at 11.517 kb on + strand, within GFF1968 at 11.517 kb on + strand, within GFF1968 at 11.518 kb on - strand, within GFF1968 at 11.518 kb on - strand, within GFF1968 at 11.853 kb on + strand, within GFF1968 at 11.853 kb on + strand, within GFF1968 at 11.853 kb on + strand, within GFF1968 at 11.853 kb on + strand, within GFF1968 at 11.871 kb on + strand, within GFF1968 at 11.871 kb on + strand, within GFF1968 at 11.944 kb on - strand at 12.102 kb on - strand, within GFF1969 at 12.130 kb on + strand, within GFF1969 at 12.484 kb on + strand, within GFF1969 at 12.484 kb on + strand, within GFF1969 at 12.484 kb on + strand, within GFF1969 at 12.484 kb on + strand, within GFF1969 at 12.484 kb on + strand, within GFF1969 at 12.485 kb on - strand, within GFF1969 at 12.485 kb on - strand, within GFF1969 at 12.485 kb on - strand, within GFF1969 at 12.485 kb on - strand, within GFF1969 at 12.738 kb on - strand, within GFF1969 at 12.933 kb on + strand at 12.934 kb on - strand at 12.934 kb on - strand at 12.934 kb on - strand at 12.934 kb on - strand at 12.993 kb on + strand, within GFF1970 at 12.993 kb on + strand, within GFF1970 at 12.993 kb on + strand, within GFF1970 at 12.993 kb on + strand, within GFF1970 at 12.993 kb on + strand, within GFF1970 at 12.993 kb on + strand, within GFF1970 at 12.994 kb on - strand, within GFF1970 at 12.994 kb on - strand, within GFF1970
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 10,690 + GFF1967 0.64 +0.2 10,691 - GFF1967 0.64 +0.9 10,691 - GFF1967 0.64 -0.7 10,753 + GFF1967 0.69 -0.3 10,873 + GFF1967 0.78 -0.9 10,873 + GFF1967 0.78 +1.0 10,873 + GFF1967 0.78 -2.6 10,873 + GFF1967 0.78 +2.1 10,874 - GFF1967 0.78 -1.2 10,874 - GFF1967 0.78 +0.1 10,924 + GFF1967 0.81 -0.6 10,924 + GFF1967 0.81 -0.4 10,924 + GFF1967 0.81 -0.5 10,924 + GFF1967 0.81 -1.6 10,925 - GFF1967 0.81 +0.6 10,925 - GFF1967 0.81 -0.6 10,925 - GFF1967 0.81 -1.3 11,346 + GFF1968 0.19 +1.3 11,448 + GFF1968 0.32 +1.2 11,448 + GFF1968 0.32 -0.7 11,448 + GFF1968 0.32 -0.7 11,448 + GFF1968 0.32 +0.8 11,448 + GFF1968 0.32 -0.7 11,449 - GFF1968 0.32 -2.3 11,517 + GFF1968 0.41 -0.2 11,517 + GFF1968 0.41 +0.3 11,518 - GFF1968 0.41 +0.7 11,518 - GFF1968 0.41 -1.0 11,853 + GFF1968 0.82 -0.6 11,853 + GFF1968 0.82 -0.8 11,853 + GFF1968 0.82 -0.4 11,853 + GFF1968 0.82 -0.9 11,871 + GFF1968 0.85 -0.8 11,871 + GFF1968 0.85 -0.7 11,944 - -0.4 12,102 - GFF1969 0.13 -0.8 12,130 + GFF1969 0.16 -0.9 12,484 + GFF1969 0.59 +0.3 12,484 + GFF1969 0.59 -1.1 12,484 + GFF1969 0.59 -0.7 12,484 + GFF1969 0.59 +0.7 12,484 + GFF1969 0.59 -0.2 12,485 - GFF1969 0.59 -1.1 12,485 - GFF1969 0.59 -0.2 12,485 - GFF1969 0.59 -0.1 12,485 - GFF1969 0.59 -0.0 12,738 - GFF1969 0.89 -0.1 12,933 + +2.1 12,934 - +0.0 12,934 - -0.7 12,934 - -0.5 12,934 - -0.5 12,993 + GFF1970 0.10 +0.9 12,993 + GFF1970 0.10 -0.5 12,993 + GFF1970 0.10 +0.4 12,993 + GFF1970 0.10 -0.0 12,993 + GFF1970 0.10 -0.4 12,993 + GFF1970 0.10 +0.6 12,994 - GFF1970 0.10 +0.3 12,994 - GFF1970 0.10 -0.0
Or see this region's nucleotide sequence