Strain Fitness in Variovorax sp. SCN45 around GFF1815

Experiment: M9 with calcium pantothenate 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1814 and GFF1815 overlap by 4 nucleotidesGFF1815 and GFF1816 are separated by 7 nucleotidesGFF1816 and GFF1817 are separated by 74 nucleotides GFF1814 - 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) decarboxylase (EC 4.1.1.97) / Allantoate amidohydrolase (EC 3.5.3.9), at 478,032 to 479,810 GFF1814 GFF1815 - Uricase (urate oxidase) (EC 1.7.3.3), at 479,807 to 480,769 GFF1815 GFF1816 - Transcriptional regulator, GntR family, at 480,777 to 481,451 GFF1816 GFF1817 - 5-hydroxyisourate hydrolase (EC 3.5.2.17), at 481,526 to 481,876 GFF1817 Position (kb) 479 480 481Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 478.885 kb on + strand, within GFF1814at 478.885 kb on + strand, within GFF1814at 478.886 kb on - strand, within GFF1814at 479.005 kb on + strand, within GFF1814at 479.005 kb on + strand, within GFF1814at 479.006 kb on - strand, within GFF1814at 479.032 kb on + strand, within GFF1814at 479.032 kb on + strand, within GFF1814at 479.033 kb on - strand, within GFF1814at 479.188 kb on + strand, within GFF1814at 479.473 kb on + strand, within GFF1814at 479.474 kb on - strand, within GFF1814at 479.741 kb on - strandat 479.741 kb on - strandat 479.741 kb on - strandat 479.741 kb on - strandat 480.018 kb on + strand, within GFF1815at 480.018 kb on + strand, within GFF1815at 480.018 kb on + strand, within GFF1815at 480.019 kb on - strand, within GFF1815at 480.045 kb on + strand, within GFF1815at 480.045 kb on + strand, within GFF1815at 480.045 kb on + strand, within GFF1815at 480.045 kb on + strand, within GFF1815at 480.045 kb on + strand, within GFF1815at 480.046 kb on - strand, within GFF1815at 480.046 kb on - strand, within GFF1815at 480.046 kb on - strand, within GFF1815at 480.046 kb on - strand, within GFF1815at 480.117 kb on + strand, within GFF1815at 480.117 kb on + strand, within GFF1815at 480.117 kb on + strand, within GFF1815at 480.118 kb on - strand, within GFF1815at 480.192 kb on + strand, within GFF1815at 480.192 kb on + strand, within GFF1815at 480.196 kb on - strand, within GFF1815at 480.196 kb on - strand, within GFF1815at 480.208 kb on - strand, within GFF1815at 480.223 kb on - strand, within GFF1815at 480.267 kb on + strand, within GFF1815at 480.267 kb on + strand, within GFF1815at 480.268 kb on - strand, within GFF1815at 480.268 kb on - strand, within GFF1815at 480.361 kb on - strand, within GFF1815at 480.534 kb on + strand, within GFF1815at 480.534 kb on + strand, within GFF1815at 480.534 kb on + strand, within GFF1815at 480.534 kb on + strand, within GFF1815at 480.534 kb on + strand, within GFF1815at 480.535 kb on - strand, within GFF1815at 480.535 kb on - strand, within GFF1815at 480.651 kb on + strand, within GFF1815at 480.651 kb on + strand, within GFF1815at 480.651 kb on + strand, within GFF1815at 480.651 kb on + strand, within GFF1815at 480.652 kb on - strand, within GFF1815at 480.652 kb on - strand, within GFF1815at 480.769 kb on + strandat 480.769 kb on + strandat 480.769 kb on + strandat 480.769 kb on + strandat 480.770 kb on - strandat 480.770 kb on - strandat 480.958 kb on + strand, within GFF1816at 480.959 kb on - strand, within GFF1816at 480.959 kb on - strand, within GFF1816at 481.454 kb on + strandat 481.454 kb on + strandat 481.454 kb on + strandat 481.454 kb on + strandat 481.454 kb on + strandat 481.454 kb on + strandat 481.454 kb on + strandat 481.476 kb on - strandat 481.545 kb on + strandat 481.546 kb on - strandat 481.595 kb on + strand, within GFF1817at 481.595 kb on + strand, within GFF1817at 481.596 kb on - strand, within GFF1817at 481.596 kb on - strand, within GFF1817at 481.673 kb on + strand, within GFF1817at 481.673 kb on + strand, within GFF1817at 481.703 kb on + strand, within GFF1817at 481.704 kb on - strand, within GFF1817at 481.730 kb on + strand, within GFF1817at 481.730 kb on + strand, within GFF1817

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL
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478,885 + GFF1814 0.48 -1.0
478,885 + GFF1814 0.48 -0.3
478,886 - GFF1814 0.48 +0.2
479,005 + GFF1814 0.55 -0.4
479,005 + GFF1814 0.55 -0.5
479,006 - GFF1814 0.55 +0.4
479,032 + GFF1814 0.56 -2.3
479,032 + GFF1814 0.56 -0.5
479,033 - GFF1814 0.56 +1.0
479,188 + GFF1814 0.65 -0.7
479,473 + GFF1814 0.81 -0.3
479,474 - GFF1814 0.81 -0.0
479,741 - -2.1
479,741 - -0.4
479,741 - +1.4
479,741 - -0.5
480,018 + GFF1815 0.22 +0.1
480,018 + GFF1815 0.22 -0.2
480,018 + GFF1815 0.22 +1.6
480,019 - GFF1815 0.22 -1.3
480,045 + GFF1815 0.25 -0.1
480,045 + GFF1815 0.25 +1.0
480,045 + GFF1815 0.25 +0.6
480,045 + GFF1815 0.25 +1.8
480,045 + GFF1815 0.25 -0.6
480,046 - GFF1815 0.25 -0.2
480,046 - GFF1815 0.25 +0.8
480,046 - GFF1815 0.25 +0.4
480,046 - GFF1815 0.25 +0.4
480,117 + GFF1815 0.32 -0.2
480,117 + GFF1815 0.32 -0.4
480,117 + GFF1815 0.32 -0.6
480,118 - GFF1815 0.32 +0.0
480,192 + GFF1815 0.40 -1.1
480,192 + GFF1815 0.40 +0.5
480,196 - GFF1815 0.40 -1.7
480,196 - GFF1815 0.40 -0.5
480,208 - GFF1815 0.42 -0.2
480,223 - GFF1815 0.43 +0.0
480,267 + GFF1815 0.48 -1.0
480,267 + GFF1815 0.48 -0.6
480,268 - GFF1815 0.48 -0.5
480,268 - GFF1815 0.48 -0.5
480,361 - GFF1815 0.58 +0.2
480,534 + GFF1815 0.75 -0.4
480,534 + GFF1815 0.75 -1.2
480,534 + GFF1815 0.75 -0.1
480,534 + GFF1815 0.75 -0.7
480,534 + GFF1815 0.75 -0.2
480,535 - GFF1815 0.76 -0.5
480,535 - GFF1815 0.76 +0.9
480,651 + GFF1815 0.88 -0.3
480,651 + GFF1815 0.88 +1.5
480,651 + GFF1815 0.88 -1.2
480,651 + GFF1815 0.88 +0.3
480,652 - GFF1815 0.88 -0.2
480,652 - GFF1815 0.88 +0.9
480,769 + +0.1
480,769 + +1.0
480,769 + +0.0
480,769 + +1.5
480,770 - +0.4
480,770 - -2.1
480,958 + GFF1816 0.27 +0.0
480,959 - GFF1816 0.27 -0.7
480,959 - GFF1816 0.27 +0.8
481,454 + +0.5
481,454 + -1.9
481,454 + +0.0
481,454 + -2.8
481,454 + -2.8
481,454 + -0.1
481,454 + -0.3
481,476 - -0.2
481,545 + -0.1
481,546 - -1.1
481,595 + GFF1817 0.20 +0.6
481,595 + GFF1817 0.20 -0.1
481,596 - GFF1817 0.20 -0.4
481,596 - GFF1817 0.20 -0.7
481,673 + GFF1817 0.42 -0.6
481,673 + GFF1817 0.42 -0.5
481,703 + GFF1817 0.50 -0.7
481,704 - GFF1817 0.51 +1.1
481,730 + GFF1817 0.58 -0.8
481,730 + GFF1817 0.58 +0.1

Or see this region's nucleotide sequence