Experiment: M9 with calcium pantothenate 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1811 and GFF1812 overlap by 4 nucleotides GFF1812 and GFF1813 are separated by 36 nucleotides GFF1813 and GFF1814 are separated by 17 nucleotides
GFF1811 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 474,752 to 476,101
GFF1811
GFF1812 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 476,098 to 476,751
GFF1812
GFF1813 - Acetylornithine deacetylase-like protein Acry_1162, at 476,788 to 478,014
GFF1813
GFF1814 - 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) decarboxylase (EC 4.1.1.97) / Allantoate amidohydrolase (EC 3.5.3.9), at 478,032 to 479,810
GFF1814
Position (kb) 476
477
478
479 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 475.826 kb on + strand, within GFF1811 at 475.917 kb on - strand, within GFF1811 at 475.917 kb on - strand, within GFF1811 at 475.917 kb on - strand, within GFF1811 at 475.917 kb on - strand, within GFF1811 at 476.048 kb on + strand at 476.048 kb on + strand at 476.049 kb on - strand at 476.429 kb on - strand, within GFF1812 at 476.429 kb on - strand, within GFF1812 at 476.429 kb on - strand, within GFF1812 at 476.429 kb on - strand, within GFF1812 at 476.458 kb on + strand, within GFF1812 at 476.458 kb on + strand, within GFF1812 at 476.458 kb on + strand, within GFF1812 at 476.459 kb on - strand, within GFF1812 at 476.648 kb on - strand, within GFF1812 at 476.852 kb on - strand at 476.912 kb on + strand, within GFF1813 at 476.912 kb on + strand, within GFF1813 at 476.913 kb on - strand, within GFF1813 at 476.942 kb on + strand, within GFF1813 at 476.942 kb on + strand, within GFF1813 at 476.943 kb on - strand, within GFF1813 at 476.943 kb on - strand, within GFF1813 at 476.943 kb on - strand, within GFF1813 at 476.960 kb on + strand, within GFF1813 at 476.960 kb on + strand, within GFF1813 at 476.960 kb on + strand, within GFF1813 at 476.960 kb on + strand, within GFF1813 at 477.302 kb on + strand, within GFF1813 at 477.302 kb on + strand, within GFF1813 at 477.303 kb on - strand, within GFF1813 at 477.320 kb on + strand, within GFF1813 at 477.320 kb on + strand, within GFF1813 at 477.320 kb on + strand, within GFF1813 at 477.321 kb on - strand, within GFF1813 at 477.321 kb on - strand, within GFF1813 at 477.321 kb on - strand, within GFF1813 at 477.452 kb on + strand, within GFF1813 at 477.452 kb on + strand, within GFF1813 at 477.453 kb on - strand, within GFF1813 at 477.453 kb on - strand, within GFF1813 at 477.545 kb on + strand, within GFF1813 at 477.545 kb on + strand, within GFF1813 at 477.545 kb on + strand, within GFF1813 at 477.690 kb on - strand, within GFF1813 at 477.770 kb on + strand, within GFF1813 at 477.813 kb on - strand, within GFF1813 at 477.813 kb on - strand, within GFF1813 at 477.813 kb on - strand, within GFF1813 at 478.004 kb on + strand at 478.004 kb on + strand at 478.531 kb on + strand, within GFF1814 at 478.532 kb on - strand, within GFF1814 at 478.628 kb on - strand, within GFF1814 at 478.628 kb on - strand, within GFF1814 at 478.703 kb on + strand, within GFF1814 at 478.703 kb on + strand, within GFF1814 at 478.704 kb on - strand, within GFF1814 at 478.704 kb on - strand, within GFF1814 at 478.885 kb on + strand, within GFF1814 at 478.885 kb on + strand, within GFF1814 at 478.886 kb on - strand, within GFF1814 at 479.005 kb on + strand, within GFF1814 at 479.005 kb on + strand, within GFF1814 at 479.006 kb on - strand, within GFF1814
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL remove 475,826 + GFF1811 0.80 -0.2 475,917 - GFF1811 0.86 -0.7 475,917 - GFF1811 0.86 +0.6 475,917 - GFF1811 0.86 +0.0 475,917 - GFF1811 0.86 -1.0 476,048 + +0.5 476,048 + -0.5 476,049 - -0.3 476,429 - GFF1812 0.51 -0.7 476,429 - GFF1812 0.51 -0.5 476,429 - GFF1812 0.51 -0.1 476,429 - GFF1812 0.51 -1.7 476,458 + GFF1812 0.55 +0.3 476,458 + GFF1812 0.55 -0.4 476,458 + GFF1812 0.55 -1.2 476,459 - GFF1812 0.55 -0.4 476,648 - GFF1812 0.84 +2.8 476,852 - -0.7 476,912 + GFF1813 0.10 -0.2 476,912 + GFF1813 0.10 +1.1 476,913 - GFF1813 0.10 -0.2 476,942 + GFF1813 0.13 -0.3 476,942 + GFF1813 0.13 -0.7 476,943 - GFF1813 0.13 -0.1 476,943 - GFF1813 0.13 -1.4 476,943 - GFF1813 0.13 +0.7 476,960 + GFF1813 0.14 -0.5 476,960 + GFF1813 0.14 -0.7 476,960 + GFF1813 0.14 +0.9 476,960 + GFF1813 0.14 -0.4 477,302 + GFF1813 0.42 -0.5 477,302 + GFF1813 0.42 +0.0 477,303 - GFF1813 0.42 +0.3 477,320 + GFF1813 0.43 -1.0 477,320 + GFF1813 0.43 -1.0 477,320 + GFF1813 0.43 +0.5 477,321 - GFF1813 0.43 -1.3 477,321 - GFF1813 0.43 -0.8 477,321 - GFF1813 0.43 +1.4 477,452 + GFF1813 0.54 -0.1 477,452 + GFF1813 0.54 -0.1 477,453 - GFF1813 0.54 -1.4 477,453 - GFF1813 0.54 +0.9 477,545 + GFF1813 0.62 -1.7 477,545 + GFF1813 0.62 -0.7 477,545 + GFF1813 0.62 -1.4 477,690 - GFF1813 0.74 -1.0 477,770 + GFF1813 0.80 -0.8 477,813 - GFF1813 0.84 +0.0 477,813 - GFF1813 0.84 +0.3 477,813 - GFF1813 0.84 -0.6 478,004 + -0.7 478,004 + -0.5 478,531 + GFF1814 0.28 -1.5 478,532 - GFF1814 0.28 +0.3 478,628 - GFF1814 0.34 +0.2 478,628 - GFF1814 0.34 +0.5 478,703 + GFF1814 0.38 +2.1 478,703 + GFF1814 0.38 +2.3 478,704 - GFF1814 0.38 -2.0 478,704 - GFF1814 0.38 +1.6 478,885 + GFF1814 0.48 -1.0 478,885 + GFF1814 0.48 -0.3 478,886 - GFF1814 0.48 +0.2 479,005 + GFF1814 0.55 -0.4 479,005 + GFF1814 0.55 -0.5 479,006 - GFF1814 0.55 +0.4
Or see this region's nucleotide sequence